KEGG   ENZYME: 1.17.99.4Help
Entry
EC 1.17.99.4                Enzyme                                 

Name
uracil/thymine dehydrogenase;
uracil oxidase;
uracil-thymine oxidase;
uracil dehydrogenase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With other, unknown, acceptors
BRITE hierarchy
Sysname
uracil:acceptor oxidoreductase
Reaction(IUBMB)
(1) uracil + H2O + acceptor = barbiturate + reduced acceptor [RN:R00976];
(2) thymine + H2O + acceptor = 5-methylbarbiturate + reduced acceptor [RN:R01413]
Reaction(KEGG)
Substrate
uracil [CPD:C00106];
H2O [CPD:C00001];
acceptor [CPD:C00028];
thymine [CPD:C00178]
Product
barbiturate [CPD:C00813];
reduced acceptor [CPD:C00030];
5-methylbarbiturate [CPD:C05281]
Comment
Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 3.5.2.1 (barbiturase) and EC 3.5.1.95 (N-malonylurea hydrolase). Mammals, plants and other microorganisms utilize the reductive pathway, comprising EC 1.3.1.1 [dihydrouracil dehydrogenase (NAD+)] or EC 1.3.1.2 [dihydropyrimidine dehydrogenase (NADP+)], EC 3.5.2.2 (dihydropyrimidinase) and EC 3.5.1.6 (beta-ureidopropionase), with the ultimate degradation products being an L-amino acid, NH3 and CO2 [5].
History
EC 1.17.99.4 created 1961 as EC 1.2.99.1, transferred 1984 to EC 1.1.99.19, transferred 2006 to EC 1.17.99.4
Pathway
Pyrimidine metabolism
Reference
1  [PMID:12981104]
  Authors
HAYAISHI O, KORNBERG A.
  Title
Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes.
  Journal
J. Biol. Chem. 197 (1952) 717-32.
Reference
2  [PMID:14927671]
  Authors
WANG TP, LAMPEN JO.
  Title
Metabolism of pyrimidines by a soil bacterium.
  Journal
J. Biol. Chem. 194 (1952) 775-83.
Reference
3  [PMID:14927672]
  Authors
WANG TP, LAMPEN JO.
  Title
Uracil oxidase and the isolation of barbituric acid from uracil oxidation.
  Journal
J. Biol. Chem. 194 (1952) 785-91.
Reference
4  [PMID:14955523]
  Authors
LARA FJ.
  Title
On the decomposition of pyrimidines by bacteria. II. Studies with cell-free enzyme preparations.
  Journal
J. Bacteriol. 64 (1952) 279-85.
Reference
5  [PMID:11485332]
  Authors
Soong CL, Ogawa J, Shimizu S.
  Title
Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase.
  Journal
Biochem. Biophys. Res. Commun. 286 (2001) 222-6.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9029-00-9

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