KEGG   ENZYME: 1.2.7.7Help
Entry
EC 1.2.7.7                  Enzyme                                 
Name
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy
Sysname
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)
Reaction(IUBMB)
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN:R07160]
Reaction(KEGG)
R07160;
(other) R08566 R08567
Show
Substrate
3-methyl-2-oxobutanoate [CPD:C00141];
CoA [CPD:C00010];
oxidized ferredoxin [CPD:C00139]
Product
S-(2-methylpropanoyl)-CoA;
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
This enzyme is one of four 2-oxoacid oxidoreductases that are differentiated by their abilities to oxidatively decarboxylate different 2-oxoacids and form their CoA derivatives (see also EC 1.2.7.1, pyruvate synthase; EC 1.2.7.3, 2-oxoglutarate synthase and EC 1.2.7.8, indolepyruvate ferredoxin oxidoreductase). It is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, 2-oxobutyrate and 3-methylthiopropanamine.
Pathway
Valine, leucine and isoleucine degradation
Metabolic pathways
Orthology
K00186  
2-oxoisovalerate ferredoxin oxidoreductase, alpha subunit
K00187  
2-oxoisovalerate ferredoxin oxidoreductase, beta subunit
K00188  
2-oxoisovalerate ferredoxin oxidoreductase, delta subunit
K00189  
2-oxoisovalerate ferredoxin oxidoreductase, gamma subunit
Genes
AFW: 
ABA: 
SUS: 
MMP: 
MMP1271(vorA) MMP1272(vorB) MMP1273(vorC)
MMQ: 
MMX: 
MMZ: 
MVN: 
MVO: 
MAC: 
MA2909(vorA) MA2910(vorB) MA2911(vorC)
MBA: 
Mbar_A2173 Mbar_A2174 Mbar_A2175
MMA: 
MM_3181(vorC) MM_3182(vorB) MM_3183(vorA)
MBU: 
Mbur_0605 Mbur_0606 Mbur_0607
MMH: 
Mmah_1010 Mmah_1011 Mmah_1012
MZH: 
Mzhil_0902 Mzhil_0903 Mzhil_0904
MTP: 
Mthe_0156 Mthe_0157
MTH: 
MTH703 MTH704 MTH705
MMG: 
MTBMA_c10900(vorC) MTBMA_c10910(vorB) MTBMA_c10930(vorA2)
MSI: 
Msm_0331 Msm_0332 Msm_0333
MRU: 
mru_1431(vorB) mru_1432(vorA) mru_1433(vorC)
MEL: 
Metbo_2452 Metbo_2453 Metbo_2454
MFV: 
Mfer_0416 Mfer_0417 Mfer_0506
PHO: 
PH0678 PH0679(vorD) PH0680(vorA) PH0681
PAB: 
PAB1470 PAB1471(vorD) PAB1472(vorA) PAB1473
PFU: 
PF0968 PF0969(vorA) PF0970(vorD) PF0971
TKO: 
TK1978 TK1979(vorD) TK1980(vorA) TK1981
TON: 
TON_1476 TON_1477(vorD) TON_1478(vorA) TON_1479
TGA: 
TGAM_0266(vorD)
TSI: 
TSIB_1383(vorD)
TBA: 
TERMP_01559
 » show all
Taxonomy
Reference
1  [PMID:8550513]
  Authors
Heider J, Mai X, Adams MW.
  Title
Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.
  Journal
J. Bacteriol. 178 (1996) 780-7.
  Organism
Pyrococcus furiosus [GN:pfu], Pyrococcus sp., Thermococcus litoralis, Thermococcus sp.
Reference
2  [PMID:9108258]
  Authors
Tersteegen A, Linder D, Thauer RK, Hedderich R.
  Title
Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.
  Journal
Eur. J. Biochem. 244 (1997) 862-8.
  Organism
Methanobacterium thermoautotrophicum [GN:mth]
Reference
3  [PMID:11265457]
  Authors
Schut GJ, Menon AL, Adams MW.
  Title
2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.
  Journal
Methods. Enzymol. 331 (2001) 144-58.
  Organism
Pyrococcus furiosus [GN:pfu], Thermococcus litoralis
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system