KEGG   ENZYME: 1.2.7.7Help
Entry
EC 1.2.7.7                  Enzyme                                 

Name
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase
Class
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy
Sysname
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)
Reaction(IUBMB)
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN:R07160]
Reaction(KEGG)
R07160;
(other) R08566 R08567
Show
Substrate
3-methyl-2-oxobutanoate [CPD:C00141];
CoA [CPD:C00010];
oxidized ferredoxin [CPD:C00139]
Product
S-(2-methylpropanoyl)-CoA;
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, 2-oxobutyrate and 3-methylthiopropanamine. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that oxidatively decarboxylate different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.
History
EC 1.2.7.7 created 2003
Pathway
Valine, leucine and isoleucine degradation
Metabolic pathways
Orthology
K00186  
2-oxoisovalerate ferredoxin oxidoreductase, alpha subunit
K00187  
2-oxoisovalerate ferredoxin oxidoreductase, beta subunit
K00188  
2-oxoisovalerate ferredoxin oxidoreductase, delta subunit
K00189  
2-oxoisovalerate ferredoxin oxidoreductase, gamma subunit
Genes
PEF: 
DEJ: 
DML: 
AFW: 
DAV: 
PBU: 
PBAS: 
SLR: 
ABA: 
SUS: 
CABY: 
MMP: 
MMP1271(vorA) MMP1272(vorB) MMP1273(vorC)
MMQ: 
MMX: 
MMZ: 
MMD: 
MMAK: 
MMAO: 
MVN: 
MVO: 
MAC: 
MA_2909(vorA) MA_2910(vorB) MA_2911(vorC)
MBA: 
MBY: 
MBW: 
MBAR: 
MBAK: 
MMA: 
MM_3181(vorC) MM_3182(vorB) MM_3183(vorA)
MMAZ: 
MMJ: 
MMAC: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MBU: 
MMET: 
MMH: 
MHAZ: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MCON_0778(vorA) MCON_0779(vorB)
MHI: 
MTH: 
MMG: 
METC: 
MWO: 
MSI: 
MRU: 
mru_1431(vorB) mru_1432(vorA) mru_1433(vorC)
MMIL: 
sm9_0692(vorB) sm9_0694(vorC)
MEYE: 
MOL: 
MEL: 
MEW: 
METH: 
MFC: 
BRM9_2123(vorB) BRM9_2125(vorC)
MFI: 
MCUB: 
MCBB_0193(vorC) MCBB_0195(vorA)
MFV: 
TAC: 
TVO: 
TVG0871982(TVG0871982) TVG0872869(TVG0872869)
MER: 
MEAR: 
PHO: 
PH0678(PH0678) PH0679(PH0679) PH0680(PH0680) PH0681(PH0681)
PAB: 
PFU: 
PFI: 
PYN: 
PYA: 
PYS: 
PYC: 
TKO: 
TON: 
TGA: 
TGAM_0264(vorB) TGAM_0265(vorA) TGAM_0266(vorD)
TSI: 
TBA: 
THE: 
THA: 
THM: 
TLT: 
THS: 
TNU: 
TEU: 
TGY: 
THV: 
TCH: 
TPEP: 
TPIE: 
PPAC: 
ABI: 
ACF: 
KCR: 
 » show all
Taxonomy
Reference
1  [PMID:8550513]
  Authors
Heider J, Mai X, Adams MW.
  Title
Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.
  Journal
J. Bacteriol. 178 (1996) 780-7.
Reference
2  [PMID:9108258]
  Authors
Tersteegen A, Linder D, Thauer RK, Hedderich R.
  Title
Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.
  Journal
Eur. J. Biochem. 244 (1997) 862-8.
Reference
3  [PMID:11265457]
  Authors
Schut GJ, Menon AL, Adams MW.
  Title
2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.
  Journal
Methods. Enzymol. 331 (2001) 144-58.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system