KEGG   ENZYME: 1.21.3.2Help
Entry
EC 1.21.3.2                 Enzyme                                 

Name
columbamine oxidase;
berberine synthase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
BRITE hierarchy
Sysname
columbamine:oxygen oxidoreductase (cyclizing)
Reaction(IUBMB)
2 columbamine + O2 = 2 berberine + 2 H2O [RN:R00044]
Reaction(KEGG)
Substrate
columbamine [CPD:C01795];
O2 [CPD:C00007]
Product
berberine [CPD:C00757];
H2O [CPD:C00001]
Comment
An iron protein. Oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine.
History
EC 1.21.3.2 created 1989 as EC 1.1.3.26, transferred 2002 to EC 1.21.3.2
Pathway
Isoquinoline alkaloid biosynthesis
Biosynthesis of secondary metabolites
Reference
1
  Authors
Rueffer, M. and Zenk, M.H.
  Title
Berberine synthesis, the methylenedioxy group forming enzyme in berberine synthesis.
  Journal
Tetrahedron Lett. 26 (1985) 201-202.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
95329-18-3

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