KEGG   ENZYME: 1.21.4.2Help
Entry
EC 1.21.4.2                 Enzyme                                 

Name
glycine reductase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With a disulfide as acceptor
BRITE hierarchy
Sysname
acetyl-phosphate ammonia:thioredoxin disulfide oxidoreductase (glycine-forming)
Reaction(IUBMB)
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin [RN:R07226]
Reaction(KEGG)
Substrate
acetyl phosphate [CPD:C00227];
NH3 [CPD:C00014];
thioredoxin disulfide [CPD:C00343];
H2O [CPD:C00001]
Product
glycine [CPD:C00037];
phosphate [CPD:C00009];
thioredoxin [CPD:C00342]
Comment
The reaction is observed only in the direction of glycine reduction. The enzyme from Eubacterium acidaminophilum consists of subunits A, B and C. Subunit B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Subunit A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by subunit C to produce acetyl phosphate. Only subunit B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase).
History
EC 1.21.4.2 created 2003
Orthology
K10670  
glycine reductase
Genes
EFE: 
PPR: 
DDS: 
SAT: 
DTI: 
LGR: 
LGV: 
SGO: 
EFA: 
EFL: 
EFI: 
EFD: 
EFS: 
EFN: 
EFQ: 
EHR: 
ECAS: 
EMU: 
CML: 
CBO: 
CBO1256(grdE) CBO1257(grdB)
CBA: 
CLB_1284(grdE) CLB_1285(grdB)
CBH: 
CLC_1296(grdE) CLC_1297(grdB)
CBY: 
CLM_1415(grdE) CLM_1416(grdB)
CBL: 
CLK_0696(grdE) CLK_0697(grdB)
CBB: 
CLD_3309(grdB) CLD_3311(grdE)
CBI: 
CBF: 
CLI_1342(grdE) CLI_1343(grdB)
CBM: 
CBJ: 
CLJ: 
AMT: 
AOE: 
CSH: 
CSO: 
CDF: 
CDC: 
CDL: 
CDG: 
CST: 
CLOST_1056(grdI) CLOST_1057(grdH) CLOST_1110(grdE) CLOST_1113(grdB)
STH: 
DOR: 
FMA: 
EAC: 
BPRM: 
TTE: 
TTE1874(PlsX2) TTE1875(FabH4) TTE1879
CHY: 
CHY_2391(grdE) CHY_2392(grdA) CHY_2393(grdB) CHY_2394(grdC)
TEP: 
TAE: 
TOC: 
TTM: 
TTO: 
TXY: 
TSH: 
CPO: 
NTH: 
HPK: 
AAR: 
HHL: 
SHI: 
TDE: 
TDE0239(grdD) TDE0240(grdC) TDE0745(grdA) TDE2119(grdB-2) TDE2120(grdE-2)
TPED: 
TPK: 
TRM: 
BRM: 
BPO: 
BPJ: 
BPIP: 
BPW: 
WESB_0748(grdE) WESB_0751(grdA1)
TAI: 
ACO: 
TLI: 
AMO: 
SBR: 
 » show all
Taxonomy
Reference
1  [PMID:10091582]
  Authors
Wagner M, Sonntag D, Grimm R, Pich A, Eckerskorn C, Sohling B, Andreesen JR.
  Title
Substrate-specific selenoprotein B of glycine reductase from Eubacterium acidaminophilum. Biochemical and molecular analysis.
  Journal
Eur. J. Biochem. 260 (1999) 38-49.
  Sequence
Reference
2  [PMID:11422384]
  Authors
Bednarski B, Andreesen JR, Pich A.
  Title
In vitro processing of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: formation of a pyruvoyl group from a cysteine residue.
  Journal
Eur. J. Biochem. 268 (2001) 3538-44.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
39307-24-9

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