KEGG   ENZYME: 1.21.4.2Help
Entry
EC 1.21.4.2                 Enzyme                                 

Name
glycine reductase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With a disulfide as acceptor
BRITE hierarchy
Sysname
acetyl-phosphate ammonia:thioredoxin disulfide oxidoreductase (glycine-forming)
Reaction(IUBMB)
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin [RN:R07226]
Reaction(KEGG)
Substrate
acetyl phosphate [CPD:C00227];
NH3 [CPD:C00014];
thioredoxin disulfide [CPD:C00343];
H2O [CPD:C00001]
Product
glycine [CPD:C00037];
phosphate [CPD:C00009];
thioredoxin [CPD:C00342]
Comment
The reaction is observed only in the direction of glycine reduction. The enzyme from Eubacterium acidaminophilum consists of subunits A, B and C. Subunit B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Subunit A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by subunit C to produce acetyl phosphate. Only subunit B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase).
History
EC 1.21.4.2 created 2003
Orthology
K10670  
glycine/sarcosine/betaine reductase complex component A
K10671  
glycine reductase complex component B subunit alpha and beta
K10672  
glycine reductase complex component B subunit gamma
K21576  
glycine/sarcosine/betaine reductase complex component C subunit alpha
K21577  
glycine/sarcosine/betaine reductase complex component C subunit beta
Genes
EFE: 
PPR: 
PGB: 
DDS: 
DFI: 
DPG: 
SAT: 
DTI: 
CBO: 
CBO1256(grdE) CBO1264(grdD)
CBA: 
CLB_1284(grdE) CLB_1285(grdB) CLB_1290(grdA) CLB_1291(grdC) CLB_1292(grdD)
CBH: 
CLC_1296(grdE) CLC_1297(grdB) CLC_1301(grdC) CLC_1302(grdD)
CBY: 
CLM_1415(grdE) CLM_1416(grdB) CLM_1420(grdA) CLM_1421(grdA) CLM_1422(grdC) CLM_1423(grdD)
CBL: 
CLK_0696(grdE) CLK_0697(grdB) CLK_0701(grdA) CLK_0702 CLK_0703(grdC) CLK_0704(grdD)
CBB: 
CLD_3303(grdD) CLD_3304(grdC) CLD_3305(grdA) CLD_3306 CLD_3309(grdB) CLD_3311(grdE)
CBI: 
CBF: 
CLI_1342 CLI_1343(grdB) CLI_1346(grdA) CLI_1347(grdC) CLI_1348(grdD)
CBM: 
CBF_1315 CBF_1319(grdA) CBF_1320(grdA) CBF_1321(grdC) CBF_1322(grdD)
CBJ: 
CLJ: 
CSQ: 
CLD: 
CACE: 
CCK: 
CFM: 
AMT: 
AOE: 
GFE: 
LACY: 
CSO: 
CDF: 
PDC: 
CDC: 
CD196_2191(grdD) CD196_2192(grdC) CD196_2193(grdB) CD196_2195(grdE)
CDL: 
PDF: 
EAC: 
EAL2_808p07350(grdA4) EAL2_c02950(grdC2) EAL2_c02960(grdD2) EAL2_c08730(grdE1) EAL2_c08740(grdA2) EAL2_c08750(grdB2) EAL2_c16950(grdD) EAL2_c16960(grdC) EAL2_c16970(grdA1) EAL2_c17000(grdB1) EAL2_c17010(grdA3) EAL2_c17020(grd2)
CST: 
CLOST_1110(grdE) CLOST_1112(grdA) CLOST_1113(grdB) CLOST_1115(grdC) CLOST_1116(grdD)
STH: 
DOR: 
BPRM: 
TTE: 
TTE1874(PlsX2) TTE1875(FabH4) TTE1879
CHY: 
CHY_2391(grdE) CHY_2392(grdA) CHY_2393(grdB) CHY_2394(grdC) CHY_2395(grdD)
TOC: 
CPO: 
NTH: 
HPK: 
AAR: 
HHL: 
FMA: 
PMIC: 
SHI: 
TDE: 
TDE0078(grdB-1) TDE0239(grdD) TDE0240(grdC) TDE0745(grdA) TDE2119(grdB-2) TDE2120(grdE-2)
TPED: 
TPK: 
TRM: 
BRM: 
BPO: 
BPJ: 
BPIP: 
BPW: 
LEO: 
TAI: 
ACO: 
TLI: 
AMO: 
SBR: 
CPOR: 
BARC: 
 » show all
Taxonomy
Reference
1  [PMID:10091582]
  Authors
Wagner M, Sonntag D, Grimm R, Pich A, Eckerskorn C, Sohling B, Andreesen JR.
  Title
Substrate-specific selenoprotein B of glycine reductase from Eubacterium acidaminophilum. Biochemical and molecular analysis.
  Journal
Eur. J. Biochem. 260 (1999) 38-49.
  Sequence
Reference
2  [PMID:11422384]
  Authors
Bednarski B, Andreesen JR, Pich A.
  Title
In vitro processing of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: formation of a pyruvoyl group from a cysteine residue.
  Journal
Eur. J. Biochem. 268 (2001) 3538-44.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
39307-24-9

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