| Entry |
|
|---|
| Name |
sarcosine reductase |
|---|
| Class |
Oxidoreductases;
Acting on X-H and Y-H to form an X-Y bond;
With a disulfide as acceptor
 |
|---|
| Sysname |
acetyl-phosphate methylamine:thioredoxin disulfide oxidoreductase
(N-methylglycine-forming) |
|---|
| Reaction(IUBMB) |
acetyl phosphate + methylamine + thioredoxin disulfide =
N-methylglycine + phosphate + thioredoxin [RN:R07227] |
|---|
| Reaction(KEGG) |
R07227
 |
|---|
| Substrate |
acetyl phosphate [CPD:C00227];
methylamine [CPD:C00218];
thioredoxin disulfide [CPD:C00343] |
|---|
| Product |
N-methylglycine [CPD:C00213];
phosphate [CPD:C00009];
thioredoxin [CPD:C00342] |
|---|
| Comment |
The reaction is observed only in the direction of sarcosine
reduction. The enzyme from Eubacterium acidaminophilum consists of
subunits A, B and C. Subunit B contains selenocysteine and a
pyruvoyl group, and is responsible for sarcosine binding and
methylamine release. Subunit A, which also contains selenocysteine,
is reduced by thioredoxin, and is needed to convert the
carboxymethyl group into a ketene equivalent, in turn used by
subunit C to produce acetyl phosphate. Only subunit B distinguishes
this enzyme from EC 1.21.4.2 (glycine reductase) and EC 1.21.4.4
(betaine reductase). |
|---|
| Orthology |
K10671 sarcosine reductase |
|---|
| Genes |
PPR: PBPRB1546 PBPRB1547 PBPRB1548 PBPRB1549
SAT: SYN_00804 SYN_00806
CDF: CD2349(grdC) CD2352(grdA)
AMT: Amet_3590 Amet_3592
STH: STH2865 STH2869
FMA: FMG_0425 FMG_0428
TTE: TTE1874(plsX2) TTE1875(fabH4)
CHY: CHY_2392(grdA) CHY_2394(grdC)
TDE: TDE0239(grdD) TDE0240(grdC) TDE0745(grdA)
TAI: Taci_1134 Taci_1135 Taci_1137
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:10091582]
Wagner M, Sonntag D, Grimm R, Pich A, Eckerskorn C, Sohling B,
Andreesen JR.
Substrate-specific selenoprotein B of glycine reductase from
Eubacterium acidaminophilum. Biochemical and molecular analysis.
Eur. J. Biochem. 260 (1999) 38-49.
Eubacterium acidaminophilum |
|---|
Reference
Authors
Title
Journal
|
2
Hormann, K. and Andreesen, J.R.
Reductive cleavage of sarcosine and betaine by Eubacterium
acidaminophilum via enzyme systems different from glycine reductase.
Arch. Microbiol. 153 (1989) 50-59. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.21.4.3
IUBMB Enzyme Nomenclature: 1.21.4.3
ExPASy - ENZYME nomenclature database: 1.21.4.3
BRENDA, the Enzyme Database: 1.21.4.3
CAS: 125752-88-7 |
|---|
