KEGG   ENZYME: 1.3.1.5Help
Entry
EC 1.3.1.5                  Enzyme                                 
Name
cucurbitacin Delta23-reductase;
NAD(P)H: cucurbitacin B Delta23-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
23,24-dihydrocucurbitacin:NAD(P)+ Delta23-oxidoreductase
Reaction(IUBMB)
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+ [RN:R03763 R03764]
Reaction(KEGG)
Substrate
23,24-dihydrocucurbitacin B;
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
cucurbitacin B [CPD:C08794];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Requires Mn2+. Fe2+ or Zn2+ can replace Mn2+ to some extent.
Reference
1  [PMID:4384331]
  Authors
Schabort JC, Potgieter DJ.
  Title
Cucurbitacin B delta 23-reductase from Cucurbita maxima. II. Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics.
  Journal
Biochim. Biophys. Acta. 151 (1968) 47-53.
  Organism
Cucurbita maxima
Reference
2  [PMID:5640163]
  Authors
Schabort JC, Potgieter DJ, de Villiers V.
  Title
Cucurbitacin B delta 23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification.
  Journal
Biochim. Biophys. Acta. 151 (1968) 33-46.
  Organism
Cucurbita maxima
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37256-38-5
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