KEGG   ENZYME: 1.3.1.85Help
Entry
EC 1.3.1.85                 Enzyme                                 

Name
crotonyl-CoA carboxylase/reductase;
CCR;
crotonyl-CoA reductase (carboxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating)
Reaction(IUBMB)
(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ [RN:R09291]
Reaction(KEGG)
Substrate
(2S)-ethylmalonyl-CoA [CPD:C18026];
NADP+ [CPD:C00006]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation).
History
EC 1.3.1.85 created 2011
Pathway
Glyoxylate and dicarboxylate metabolism
Microbial metabolism in diverse environments
Orthology
K14446  
crotonyl-CoA carboxylase/reductase
Genes
ECA: 
ECA0600(cfa8B)
PATR: 
PAY: 
PST: 
BAM: 
APP: 
ANT: 
DDS: 
PLA: 
XAU: 
AZC: 
MEX: 
MEA: 
MDI: 
MCH: 
MRD: 
MET: 
MPO: 
MNO: 
HDN: 
HDT: 
HMC: 
HNI: 
RVA: 
MSC: 
CCR: 
CCS: 
CAK: 
CSE: 
SIL: 
SPO0370(ccrA)
SIT: 
RSP: 
RSH: 
RSQ: 
RSK: 
RCP: 
JAN: 
RDE: 
RD1_1637(ccrA)
RLI: 
PDE: 
PAMI: 
DSH: 
PSF: 
PGA: 
PGL: 
PGD: 
OAT: 
OAR: 
LMD: 
RED: 
PTP: 
HNE: 
HNE_3118(ccrA)
HBA: 
RRU: 
RRF: 
RPM: 
MAG: 
MGY: 
TMO: 
PGV: 
CSR: 
RCH: 
ROP: 
SMA: 
SAV_414(pteB)
SBH: 
SALB: 
SRC: 
NDA: 
NAL: 
CWO: 
LIL: 
LA_0296(adhP)
LIE: 
LIF_A0249(adhP)
LIC: 
LBJ: 
LBL: 
LBI: 
LBF: 
TPX: 
 » show all
Taxonomy
Reference
1  [PMID:17548827]
  Authors
Erb TJ, Berg IA, Brecht V, Muller M, Fuchs G, Alber BE
  Title
Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 10631-6.
  Sequence
[rsp:RSP_0960]
Reference
2  [PMID:19458256]
  Authors
Erb TJ, Brecht V, Fuchs G, Muller M, Alber BE
  Title
Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 8871-6.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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