KEGG   ENZYME: 1.3.1.85Help
Entry
EC 1.3.1.85                 Enzyme                                 
Name
crotonyl-CoA carboxylase/reductase;
CCR;
crotonyl-CoA reductase (carboxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating)
Reaction(IUBMB)
(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ [RN:R09291]
Reaction(KEGG)
Substrate
(2S)-ethylmalonyl-CoA [CPD:C18026];
NADP+ [CPD:C00006]
Product
(E)-but-2-enoyl-CoA;
CO2 [CPD:C00011];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation).
Pathway
Glyoxylate and dicarboxylate metabolism
Microbial metabolism in diverse environments
Orthology
K14446  
crotonyl-CoA carboxylase/reductase
Genes
ECA: 
ECA0600(cfa8B)
PAY: 
PST: 
BAM: 
APP: 
ANT: 
DDS: 
PLA: 
XAU: 
AZC: 
MEX: 
Mext_0288
MEA: 
Mex_1p0178(ccr)
MDI: 
METDI0161(ccr)
MCH: 
Mchl_0332
MRD: 
Mrad2831_1105
MET: 
M446_0037
MPO: 
Mpop_0364
MNO: 
Mnod_0039
HDN: 
Hden_0216
HDT: 
HYPDE_23643
HMC: 
HYPMC_0098(ccr)
RVA: 
Rvan_2964
MSC: 
BN69_1948
CCR: 
CC_3085
CCS: 
CCNA_03181
CAK: 
Caul_1067
CSE: 
Cseg_3646
SIL: 
SPO0370(ccrA)
SIT: 
TM1040_3104
RSP: 
RSP_0960
RSH: 
Rsph17029_2620
RSQ: 
Rsph17025_3009
RSK: 
RSKD131_2352
RCP: 
RCAP_rcc03243(ccrA)
JAN: 
Jann_0793
RDE: 
RD1_1637(ccrA)
RLI: 
RLO149_c030710(ccr)
PDE: 
Pden_3873
DSH: 
Dshi_2853
PSF: 
PSE_0115
PGA: 
PGA1_c03870
PGL: 
PGA2_c03420
OAT: 
OAN307_c41350(ccr)
OAR: 
OA238_c04430(ccr)
HNE: 
HNE_3118(ccrA)
HBA: 
Hbal_0288
RRU: 
Rru_A3063
RRF: 
F11_15690
RPM: 
RSPPHO_00638
MAG: 
amb0309
TMO: 
TMO_0119
PGV: 
SL003B_0355
CSR: 
Cspa_c03820(ccr)
RCH: 
RUM_00790
ROP: 
ROP_72100
TPR: 
Tpau_3474
SCO: 
SCO6473(SC9C7.09c)
SMA: 
SAV_414(pteB)
SGR: 
SGR_1170
SCB: 
SCAB_17621
SSX: 
SACTE_5660
SVL: 
Strvi_2501
SCT: 
SCAT_0122 SCAT_4859(ccr) SCAT_5748(ccrA)
SCY: 
SCATT_01300 SCATT_48530 SCATT_57510
SFA: 
Sfla_0905
SBH: 
SBI_01373 SBI_02836
SHY: 
SHJG_7434
SHO: 
SHJGH_7194
SVE: 
SVEN_6307
SDV: 
BN159_1952
SALB: 
XNR_0456 XNR_5889
STRP: 
F750_5949
SFI: 
SFUL_6327
KSK: 
KSE_56510(ccr1) KSE_65530(ccr3) KSE_70410(ccr2)
ICA: 
Intca_2418
NCA: 
Noca_1795
KFL: 
Kfla_4989
NDA: 
Ndas_0488
NAL: 
B005_2057 B005_3356
TCU: 
Tcur_4755
SRO: 
Sros_8814
FRA: 
Francci3_3690
FRE: 
Franean1_3014
FRI: 
FraEuI1c_0956
FAL: 
FRAAL5913
FSY: 
FsymDg_3826
GOB: 
Gobs_4114
BSD: 
BLASA_3898
AMD: 
AMED_1574(qor)
AMN: 
RAM_07995
AMM: 
AMES_1564(qor)
PDX: 
Psed_1713
AMI: 
Amir_3664
SESP: 
BN6_73120
STP: 
Strop_3612
SAQ: 
Sare_3994
MAU: 
Micau_5101
MIL: 
ML5_3210
CAI: 
Caci_1252
SNA: 
Snas_4138
CWO: 
Cwoe_4182
AFO: 
Afer_0105
AYM: 
YM304_37120
LIL: 
LA_0296(adhP)
LIE: 
LIF_A0249(adhP)
LIC: 
LIC10253
LBJ: 
LBJ_2607
LBL: 
LBL_0505
LBI: 
LEPBI_I3117
LBF: 
LBF_3008
TPX: 
Turpa_4007
 » show all
Taxonomy
Reference
1  [PMID:17548827]
  Authors
Erb TJ, Berg IA, Brecht V, Muller M, Fuchs G, Alber BE
  Title
Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 10631-6.
  Organism
Rhodobacter sphaeroides [GN:rsp]
Reference
2  [PMID:19458256]
  Authors
Erb TJ, Brecht V, Fuchs G, Muller M, Alber BE
  Title
Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 8871-6.
  Organism
Rhodobacter sphaeroides
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system