KEGG   ENZYME: 1.3.3.8Help
Entry
EC 1.3.3.8                  Enzyme                                 
Name
tetrahydroberberine oxidase;
(S)-THB oxidase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
(S)-tetrahydroberberine:oxygen oxidoreductase
Reaction(IUBMB)
(S)-tetrahydroberberine + 2 O2 = berberine + 2 H2O2 [RN:R02880]
Reaction(KEGG)
R02880;
(other) R03722 R08795
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Substrate
(S)-tetrahydroberberine [CPD:C03329];
O2 [CPD:C00007]
Product
berberine [CPD:C00757];
H2O2 [CPD:C00027]
Comment
The enzyme from Berberis sp. is a flavoprotein; that from Coptis japonica is not. (R)-Tetrahydroberberines are not oxidized.
Pathway
Isoquinoline alkaloid biosynthesis
Biosynthesis of secondary metabolites
Reference
1
  Authors
Amann, M., Nagakura, N. and Zenk, M.H.
  Title
(S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis.
  Journal
Tetrahedron Lett. 25 (1984) 953-954.
Reference
2
  Authors
Okada, N., Shinmyo, A., Okada, H. and Yamada, Y.
  Title
Purification and characterization of (S)-tetrahydroberberine oxidase from cultured Coptis japonica cells.
  Journal
Phytochemistry 27 (1988) 979-982.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
114705-00-9
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