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Entry
EC 1.3.5.5                  Enzyme                                 

Name
15-cis-phytoene desaturase;
phytoene desaturase (ambiguous);
PDS;
plant-type phytoene desaturase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With a quinone or related compound as acceptor
BRITE hierarchy
Sysname
15-cis-phytoene:plastoquinone oxidoreductase
Reaction(IUBMB)
15-cis-phytoene + 2 plastoquinone = 9,15,9'-tricis-zeta-carotene + 2 plastoquinol (overall reaction) [RN:R09652];
(1a) 15-cis-phytoene + plastoquinone = 15,9'-dicis-phytofluene + plastoquinol [RN:R09653];
(1b) 15,9'-dicis-phytofluene + plastoquinone = 9,15,9'-tricis-zeta-carotene + plastoquinol [RN:R09654]
Reaction(KEGG)
Substrate
15-cis-phytoene [CPD:C05421];
plastoquinone [CPD:C02061];
15,9'-dicis-phytofluene [CPD:C19765]
Product
9,15,9'-tricis-zeta-carotene [CPD:C19764];
plastoquinol [CPD:C16693];
15,9'-dicis-phytofluene [CPD:C19765]
Comment
This enzyme is involved in carotenoid biosynthesis in plants and cyanobacteria. The enzyme from Synechococcus can also use NAD+ and NADP+ as electron acceptor under anaerobic conditions. The enzyme from Gentiana lutea shows no activity with NAD+ or NADP+ [1].
History
EC 1.3.5.5 created 2011
Pathway
Carotenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K02293  
15-cis-phytoene desaturase
Genes
ATH: 
AT4G14210(PDS3)
ALY: 
CRB: 
EUS: 
CIT: 
102577966(CitPDS)
CIC: 
TCC: 
GMX: 
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
POPTR_0014s14610g(POPTRDRAFT_1099624)
VVI: 
100240685(PDS1)
SLY: 
544073(pds)
SOT: 
OSA: 
DOSA: 
Os03t0184000-00(Os03g0184000)
OBR: 
BDI: 
ZMA: 
542329(pds1)
SITA: 
ATR: 
s00131p00071060(AMTR_s00131p00071060)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
MIS: 
MPP: 
CVR: 
CME: 
PTI: 
TPS: 
EHX: 
GTT: 
GPB: 
VPE: 
BSUB: 
PMS: 
PMQ: 
OTE: 
CTM: 
SYN: 
slr1254(pds)
SYZ: 
SYY: 
SYT: 
SYS: 
SYQ: 
SYW: 
SYNW2257(crtD)
SYC: 
SYF: 
SYD: 
SYE: 
SYG: 
SYR: 
SYX: 
SYP: 
CYA: 
CYB: 
SYNE: 
SYNP: 
TEL: 
tll1561(pds)
THN: 
MAR: 
CYT: 
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
AMR: 
AM1_4826(crtP)
CGC: 
CAN: 
CSN: 
DSL: 
HAO: 
CMP: 
CYU: 
ANA: 
alr1832(pds)
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
PMA: 
PMM: 
PMM0144(pds)
PMT: 
PMT2004(pds)
PMN: 
PMI: 
PMB: 
PMC: 
PMF: 
PMG: 
PMH: 
PMJ: 
PME: 
TER: 
LEP: 
GEI: 
OAC: 
ONI: 
PSEU: 
CEP: 
MIC: 
ARP: 
CTHE: 
PLP: 
SCS: 
CTE: 
CPC: 
CCH: 
CPH: 
CPB: 
CLI: 
PVI: 
PLT: 
PPH: 
PAA: 
CTS: 
CAU: 
CAG: 
CHL: 
 » show all
Taxonomy
Reference
1  [PMID:11714315]
  Authors
Breitenbach J, Zhu C, Sandmann G
  Title
Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with  the cofactors.
  Journal
J. Agric. Food. Chem. 49 (2001) 5270-2.
  Organism
Synechococcus sp., Gentiana lutea
Reference
2  [PMID:9226712]
  Authors
Schneider C, Boger P, Sandmann G
  Title
Phytoene desaturase: heterologous expression in an active state, purification, and biochemical properties.
  Journal
Protein. Expr. Purif. 10 (1997) 175-9.
  Organism
Synechococcus sp.
  Sequence
Reference
3  [PMID:8489496]
  Authors
Fraser PD, Linden H, Sandmann G
  Title
Purification and reactivation of recombinant Synechococcus phytoene desaturase from an overexpressing strain of Escherichia coli.
  Journal
Biochem. J. 291 ( Pt 3) (1993) 687-92.
  Organism
Synechococcus sp.
Reference
4  [PMID:15503129]
  Authors
Breitenbach J, Sandmann G
  Title
zeta-Carotene cis isomers as products and substrates in the plant poly-cis carotenoid biosynthetic pathway to lycopene.
  Journal
Planta. 220 (2005) 785-93.
  Organism
Capsicum annuum
  Sequence
[up:P80093]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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