KEGG   ENZYME: 1.3.99.27Help
Entry
EC 1.3.99.27                Enzyme                                 

Name
1-hydroxycarotenoid 3,4-desaturase;
CrtD;
hydroxyneurosporene desaturase;
carotenoid 3,4-dehydrogenase;
1-hydroxy-carotenoid 3,4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other, unknown, acceptors
BRITE hierarchy
Sysname
1-hydroxy-1,2-dihydrolycopene:acceptor oxidoreductase
Reaction(IUBMB)
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor [RN:R07520]
Reaction(KEGG)
R07520;
(other) R07523 R07534
Show
Substrate
1-hydroxy-1,2-dihydrolycopene [CPD:C19795];
acceptor [CPD:C00028]
Product
1-hydroxy-3,4-didehydro-1,2-dihydrolycopene [CPD:C15874];
reduced acceptor [CPD:C00030]
Comment
The enzymes from Rubrivivax gelatinosus and Rhodobacter sphaeroides prefer the acyclic carotenoids (e.g. 1-hydroxy-1,2-dihydroneurosporene, 1-hydroxy-1,2-dihydrolycopene) as substrates. The conversion rate for the 3,4-desaturation of the monocyclic 1'-hydroxy-1',2'-dihydro-gamma-carotene is lower [2,3]. The enzyme from the marine bacterium strain P99-3 shows high activity with the monocyclic carotenoid 1'-hydroxy-1',2'-dihydro-gamma-carotene [1]. The enzyme from Rhodobacter sphaeroides utilizes molecular oxygen as the electron acceptor in vitro [3]. However, oxygen is unlikely to be the natural electron acceptor under anaerobic conditions.
History
EC 1.3.99.27 created 2011
Pathway
Carotenoid biosynthesis
Metabolic pathways
Orthology
K09845  
1-hydroxycarotenoid 3,4-desaturase
Genes
ALV: 
TVI: 
TMB: 
HHA: 
HHC: 
RGE: 
RGE_33590(crtD)
SCL: 
sce0585(crtD)
SCU: 
BRA: 
BRADO1614(crtD)
BBT: 
BBta_6441(crtD)
BRS: 
S23_19140(crtD)
AOL: 
RPA: 
RPA1518(crtD)
RPB: 
RPC: 
RPD: 
RPE: 
RPT: 
RPX: 
MEX: 
MEA: 
MDI: 
MCH: 
MRD: 
MET: 
MPO: 
MOR: 
MSL: 
RVA: 
BSB: 
RSP: 
RSP_0266(crtD)
RSH: 
RSQ: 
RSK: 
RCP: 
JAN: 
RDE: 
RD1_0115(crtD)
RLI: 
DSH: 
Dshi_3513(crtD)
RED: 
PTP: 
AMV: 
RRU: 
RRF: 
RCE: 
RC1_2090(crtD)
RPM: 
 » show all
Taxonomy
Reference
1  [PMID:15251462]
  Authors
Teramoto M, Rahlert N, Misawa N, Sandmann G.
  Title
1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol.
  Journal
FEBS. Lett. 570 (2004) 184-8.
Reference
2  [PMID:10880364]
  Authors
Steiger S, Astier C, Sandmann G
  Title
Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax  gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin.
  Journal
Biochem. J. 349 (2000) 635-40.
  Sequence
[up:Q9JPB5] [rge:RGE_33590]
Reference
3  [PMID:9393712]
  Authors
Albrecht M, Ruther A, Sandmann G.
  Title
Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides.
  Journal
J. Bacteriol. 179 (1997) 7462-7.
  Sequence
[rsp:RSP_0266]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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