KEGG   ENZYME: 1.4.7.1Help
Entry
EC 1.4.7.1                  Enzyme                                 

Name
glutamate synthase (ferredoxin);
ferredoxin-dependent glutamate synthase;
ferredoxin-glutamate synthase;
glutamate synthase (ferredoxin-dependent)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy
Sysname
L-glutamate:ferredoxin oxidoreductase (transaminating)
Reaction(IUBMB)
2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ (overall reaction) [RN:R00021];
(1a) L-glutamate + NH3 = L-glutamine + H2O [RN:R00256];
(1b) L-glutamate + 2 oxidized ferredoxin + H2O = NH3 + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ [RN:R10086]
Reaction(KEGG)
Substrate
L-glutamate [CPD:C00025];
oxidized ferredoxin [CPD:C00139];
NH3 [CPD:C00014];
H2O [CPD:C00001]
Product
L-glutamine [CPD:C00064];
2-oxoglutarate [CPD:C00026];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
H2O [CPD:C00001];
NH3 [CPD:C00014]
Comment
Binds a [3Fe-4S] cluster as well as FAD and FMN. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate. The NH3 is channeled through a 24 A channel in the active protein. No hydrolysis of glutamine takes place without ferredoxin and 2-oxoglutarate being bound to the protein [5,6].
History
EC 1.4.7.1 created 1976, modified 2012
Pathway
Glyoxylate and dicarboxylate metabolism
Nitrogen metabolism
Microbial metabolism in diverse environments
Orthology
K00284  
glutamate synthase (ferredoxin)
Genes
ATH: 
AT2G41220(GLU2) AT5G04140(GLU1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
548016(GLU)
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
POPTR_0006s03660g(POPTRDRAFT_819004) POPTR_0016s03630g(POPTRDRAFT_825050)
VVI: 
SLY: 
SOT: 
OSA: 
OBR: 
BDI: 
SBI: 
SORBI_02g041740(SORBIDRAFT_02g041740)
ZMA: 
542710(fgs1)
SITA: 
ATR: 
s00063p00191490(AMTR_s00063p00191490)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CME: 
CymeCp051(gltB)
GSL: 
PTI: 
TPS: 
GTT: 
BUR: 
BCN: 
BCH: 
BCM: 
BCJ: 
BCAL0289(glt1)
BAM: 
BAC: 
BMU: 
BMJ: 
BGL: 
BGD: 
RFR: 
PNA: 
VEI: 
VAP: 
VPE: 
GLO: 
GBM: 
Gbem_2866(gltB)
GEM: 
GEB: 
ADE: 
ACP: 
AFW: 
ANK: 
RSQ: 
BSUB: 
BHA: 
BH1728(gltA)
BCE: 
BCZ: 
BCZK3348(gltB)
BTK: 
BCL: 
ABC2035(gltA)
BPF: 
OIH: 
PJD: 
GYM: 
PPY: 
PPM: 
PPO: 
PPM_1088(gltA)
PMS: 
PMW: 
PTA: 
TCO: 
CBK: 
CBT: 
CBE: 
CCB: 
CCL: 
EHA: 
BPB: 
bpr_I0123(gltB)
CLE: 
RHO: 
CSH: 
DSY: 
DHD: 
DDH: 
DDL: 
DRM: 
DAE: 
DCA: 
DRU: 
PTH: 
PTH_0734(GltB)
SGY: 
DOR: 
DAI: 
DMI: 
DED: 
DEC: 
HMO: 
HM1_1037(gltB)
EEL: 
ERE: 
AWO: 
OVA: 
OBV_07580(gltB)
TMR: 
TNR: 
HAS: 
VPR: 
MED: 
MVA: 
MGI: 
NCA: 
OTE: 
CAA: 
LIL: 
LB_286(gltB2)
LIC: 
LIC20220(gltB)
CTM: 
IPA: 
SYN: 
sll1499(gltB) sll1502(gltB)
SYZ: 
MYO_130230(gltB) MYO_14470(gltB)
SYY: 
SYT: 
SYS: 
SYQ: 
SYW: 
SYNW2132(glsF)
SYC: 
syc0650_c(glsF)
SYF: 
SYD: 
SYE: 
SYG: 
SYR: 
SYX: 
SYP: 
CYA: 
CYA_2704(gltS)
CYB: 
CYB_1253(gltS)
SYNE: 
SYNP: 
TEL: 
tll1368(glsF)
THN: 
MAR: 
MAE_07560(gltB) MAE_29110(glsF)
CYT: 
cce_4353(glsF)
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
AMR: 
CGC: 
CAN: 
CSN: 
DSL: 
HAO: 
GLP: 
CMP: 
CYU: 
TER: 
LEP: 
GEI: 
OAC: 
ONI: 
PSEU: 
CEP: 
MIC: 
ARP: 
GVI: 
gvip210(glsF)
GLJ: 
GKIL_0118(gltS)
ANA: 
alr4344(gltS)
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
PMA: 
Pro_1668(glsF)
PMM: 
PMM1512(glsF)
PMT: 
PMT1777(glsF_gltS)
PMN: 
PMI: 
PMB: 
PMC: 
PMF: 
PMG: 
PMH: 
PMJ: 
PME: 
CTHE: 
PLP: 
SCS: 
RMR: 
GFO: 
GFO_2908(gltB)
FJO: 
FBR: 
RBI: 
ZPR: 
FBC: 
CAO: 
CLY: 
KDI: 
LAN: 
MRS: 
CTE: 
CT0401(gltB)
CPC: 
CCH: 
CPH: 
CPB: 
CLI: 
PVI: 
PLT: 
PPH: 
PAA: 
CTS: 
RRS: 
RCA: 
CAU: 
CAG: 
CHL: 
HAU: 
CAP: 
DGE: 
TRA: 
OPR: 
MHD: 
PMO: 
TTR: 
MOX: 
DAMO_0867(gltB)
 » show all
Taxonomy
Reference
1
  Authors
Galvan, F., Marquez, A.J. and Vega, J.M.
  Title
Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardii.
  Journal
Planta 162 (1984) 180-187.
Reference
2  [PMID:4423889]
  Authors
Lea PJ, Miflin BJ.
  Title
Alternative route for nitrogen assimilation in higher plants.
  Journal
Nature. 251 (1974) 614-6.
Reference
3  [PMID:12069605]
  Authors
Ravasio S, Dossena L, Martin-Figueroa E, Florencio FJ, Mattevi A, Morandi P, Curti B, Vanoni MA
  Title
Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit.
  Journal
Biochemistry. 41 (2002) 8120-33.
Reference
4  [PMID:10898944]
  Authors
Navarro F, Martin-Figueroa E, Candau P, Florencio FJ
  Title
Ferredoxin-dependent iron-sulfur flavoprotein glutamate synthase (GlsF) from the  Cyanobacterium synechocystis sp. PCC 6803: expression and assembly in Escherichia coli.
  Journal
Arch. Biochem. Biophys. 379 (2000) 267-76.
Reference
5  [PMID:11967268]
  Authors
van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A
  Title
Structural studies on the synchronization of catalytic centers in glutamate synthase.
  Journal
J. Biol. Chem. 277 (2002) 24579-83.
Reference
6  [PMID:12818206]
  Authors
van den Heuvel RH, Svergun DI, Petoukhov MV, Coda A, Curti B, Ravasio S, Vanoni MA, Mattevi A
  Title
The active conformation of glutamate synthase and its binding to ferredoxin.
  Journal
J. Mol. Biol. 330 (2003) 113-28.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
62213-56-3

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