KEGG   ENZYME: 1.4.99.5Help
Entry
EC 1.4.99.5                 Enzyme                                 
Name
glycine dehydrogenase (cyanide-forming);
hydrogen cyanide synthase;
HCN synthase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With other acceptors
BRITE hierarchy
Sysname
glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)
Reaction(IUBMB)
glycine + 2 A = hydrogen cyanide + CO2 + 2 AH2 [RN:R05704]
Reaction(KEGG)
Substrate
glycine [CPD:C00037];
A [CPD:C00028]
Product
hydrogen cyanide [CPD:C01326];
CO2 [CPD:C00011];
AH2 [CPD:C00030]
Comment
The enzyme from Pseudomonas sp. contains FAD. The enzyme is membrane-bound, and the 2-electron acceptor is a component of the respiratory chain. The enzyme can act with various artificial electron acceptors, including phenazine methosulfate.
Pathway
Cyanoamino acid metabolism
Metabolic pathways
Orthology
K10814  
hydrogen cyanide synthase HcnA
K10815  
hydrogen cyanide synthase HcnB
K10816  
hydrogen cyanide synthase HcnC
Genes
PAE: 
PA2193(hcnA) PA2194(hcnB) PA2195(hcnC)
PAU: 
PA14_36310(hcnC) PA14_36320(hcnB) PA14_36330(hcnA)
PAP: 
PSPA7_3101(hcnC) PSPA7_3102(hcnB) PSPA7_3103(hcnA)
PAG: 
PLES_31301(hcnC) PLES_31311(hcnB) PLES_31321(hcnA)
PAF: 
PAM18_2840(hcnC) PAM18_2841(hcnB) PAM18_2842(hcnA)
PNC: 
NCGM2_3175(hcnA) NCGM2_3176(hcnB) NCGM2_3177(hcnC)
PDK: 
PFL: 
PFL_2577(hcnA) PFL_2578(hcnB) PFL_2579(hcnC)
PFO: 
PFE: 
PEN: 
PSEEN5520(hcnC) PSEEN5521(hcnB) PSEEN5522(hcnA)
PBA: 
PSEBR_a3359 PSEBR_a3360 PSEBR_a3361
CVI: 
CV_1682(hcnC) CV_1683(hcnB) CV_1684(hcnA)
 » show all
Taxonomy
Reference
1  [PMID:234422]
  Authors
Wissing F.
  Title
Cyanide production from glycine by a homogenate from a Pseudomonas species.
  Journal
J. Bacteriol. 121 (1975) 695-9.
  Organism
Pseudomonas sp.
Reference
2  [PMID:233722]
  Authors
Castric PA.
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J. Bacteriol. 130 (1977) 826-31.
  Organism
Pseudomonas aeruginosa
Reference
3  [PMID:9620970]
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D.
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J. Bacteriol. 180 (1998) 3187-96.
  Organism
Pseudomonas fluorescens
Reference
4  [PMID:10763748]
  Authors
Blumer C, Haas D.
  Title
Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis.
  Journal
Arch. Microbiol. 173 (2000) 170-7.
  Organism
Pseudomonas aeruginosa, Pseudomonas fluorescens
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system