KEGG   ENZYME: 1.5.3.13Help
Entry
EC 1.5.3.13                 Enzyme                                 

Name
N1-acetylpolyamine oxidase;
hPAO-1;
PAO (ambiguous);
mPAO;
hPAO;
polyamine oxidase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
N1-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming)
Reaction(IUBMB)
(1) N1-acetylspermidine + O2 + H2O = putrescine + 3-acetamidopropanal + H2O2 [RN:R09074];
(2) N1-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2 [RN:R03899]
Reaction(KEGG)
Substrate
N1-acetylspermidine [CPD:C00612];
O2 [CPD:C00007];
H2O [CPD:C00001];
N1-acetylspermine [CPD:C02567]
Product
putrescine [CPD:C00134];
3-acetamidopropanal [CPD:C18170];
H2O2 [CPD:C00027];
spermidine [CPD:C00315]
Comment
The enzyme also catalyses the reaction: N1,N12-diacetylspermine + O2 + H2O = N1-acetylspermidine + 3-acetamamidopropanal + H2O2 [1]. No or very weak activity with spermine, or spermidine in absence of aldehydes. In presence of aldehydes the enzyme catalyses the reactions: 1. spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2, and with weak efficiency 2. spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2 [2]. A flavoprotein (FAD). This enzyme, encoded by the PAOX gene, is found in mammalian peroxisomes and oxidizes N1-acetylated polyamines at the exo (three-carbon) side of the secondary amine, forming 3-acetamamidopropanal. Since the products of the reactions are deacetylated polyamines, this process is known as polyamine back-conversion. Differs in specificity from EC 1.5.3.14 [polyamine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.15 [N8-acetylspermidine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.16 (spermine oxidase) and EC 1.5.3.17 (non-specific polyamine oxidase).
History
EC 1.5.3.13 created 2009
Orthology
K00308  
N1-acetylpolyamine oxidase
Genes
HSA: 
196743(PAOX)
PTR: 
100612198(PAOX)
PPS: 
100991166(PAOX)
GGO: 
101144152(PAOX)
PON: 
100447841(PAOX)
NLE: 
100591252(PAOX)
MCC: 
100498675(PAOX)
MCF: 
102133238(PAOX)
CJC: 
100391148(PAOX)
MMU: 
212503(Paox)
RNO: 
293589(Paox)
CGE: 
100754371(Paox)
NGI: 
103725840(Paox)
HGL: 
101697041(Paox)
OCU: 
100341296(PAOX)
TUP: 
102487097(PAOX)
AML: 
UMR: 
103659644(PAOX)
FCA: 
101090142(PAOX)
PTG: 
102954249(PAOX)
BTA: 
282639(PAOX)
BOM: 
102280842(PAOX)
PHD: 
102327990(PAOX)
CHX: 
102185531(PAOX)
OAS: 
101111594(PAOX)
SSC: 
100626281(PAOX)
CFR: 
102509887(PAOX)
BACU: 
103020663(PAOX)
LVE: 
103087537(PAOX)
ECB: 
100629322(PAOX)
MYB: 
102244775(PAOX)
MYD: 
102769158(PAOX)
PALE: 
102897106(PAOX)
MDO: 
100618593(PAOX)
SHR: 
100935074(PAOX)
OAA: 
GGA: 
100857693(PAOX)
APLA: 
101801639(PAOX)
TGU: 
100226026(PAOX)
FAB: 
101813552(PAOX)
PHI: 
102109800(PAOX)
FPG: 
101915114(PAOX)
FCH: 
102054181(PAOX)
CLV: 
102084101(PAOX)
ASN: 
102375090(PAOX)
AMJ: 
102575603(PAOX)
PSS: 
102444520(PAOX)
CMY: 
102934388(PAOX)
ACS: 
100563036(paox)
PBI: 
103061034(PAOX)
XLA: 
495472(paox)
XTR: 
496840(paox)
DRE: 
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
ISC: 
GTT: 
 » show all
Taxonomy
Reference
1  [PMID:12477380]
  Authors
Vujcic S, Liang P, Diegelman P, Kramer DL, Porter CW
  Title
Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion.
  Journal
Biochem. J. 370 (2003) 19-28.
  Sequence
[hsa:196743]
Reference
2  [PMID:15611107]
  Authors
Jarvinen A, Grigorenko N, Khomutov AR, Hyvonen MT, Uimari A, Vepsalainen J, Sinervirta R, Keinanen TA, Vujcic S, Alhonen L, Porter CW, Janne J
  Title
Metabolic stability of alpha-methylated polyamine derivatives and their use as substitutes for the natural polyamines.
  Journal
J. Biol. Chem. 280 (2005) 6595-601.
Reference
3  [PMID:15791459]
  Authors
Wang Y, Hacker A, Murray-Stewart T, Frydman B, Valasinas A, Fraser AV, Woster PM, Casero RA Jr
  Title
Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity.
  Journal
Cancer. Chemother. Pharmacol. 56 (2005) 83-90.
Reference
4  [PMID:12660232]
  Authors
Wu T, Yankovskaya V, McIntire WS
  Title
Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N1-acetylated polyamine oxidase.
  Journal
J. Biol. Chem. 278 (2003) 20514-25.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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