KEGG   ENZYME: 1.5.4.1Help
Entry
EC 1.5.4.1                  Enzyme                                 

Name
pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor
BRITE hierarchy
Sysname
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Reaction(IUBMB)
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:R03984]
Reaction(KEGG)
Substrate
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:C02587];
glutathione disulfide [CPD:C00127];
H2O [CPD:C00001]
Product
6-pyruvoyltetrahydropterin [CPD:C03684];
glutathione [CPD:C00051]
Comment
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
History
EC 1.5.4.1 created 1990, modified 2014
Pathway
Glutathione metabolism
Folate biosynthesis
Orthology
K00310  
pyrimidodiazepine synthase
Genes
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
Dsimw501_GD14099(Dsim_GD14099) Dsimw501_GD14100(Dsim_GD14100)
DWI: 
DYA: 
Dyak_GE20748(GLEANR_4570) Dyak_GE20749(GLEANR_4571)
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
552118(GstO1)
BIM: 
SOC: 
AEC: 
HST: 
CFO: 
NVI: 
100116224(GstO1)
TCA: 
BMOR: 
100141441(GSTo4) 692819(GSTo1) 692941(GSTo2) 732973(GSTo3)
PXY: 
PHU: 
DPX: 
TAD: 
 » show all
Taxonomy
Reference
1  [PMID:6438092]
  Authors
Wiederrecht GJ, Brown GM.
  Title
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.
  Journal
J. Biol. Chem. 259 (1984) 14121-7.
Reference
2  [PMID:16712527]
  Authors
Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J
  Title
Identification and characteristics of the structural gene for the Drosophila eye  colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.
  Journal
Biochem. J. 398 (2006) 451-60.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
93586-06-2

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