KEGG   ENZYME: 1.5.99.12Help
Entry
EC 1.5.99.12                Enzyme                                 
Name
cytokinin dehydrogenase;
N6-dimethylallyladenine:(acceptor) oxidoreductase;
6-N-dimethylallyladenine:acceptor oxidoreductase;
OsCKX2;
CKX;
cytokinin oxidase/dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With other acceptors
BRITE hierarchy
Sysname
N6-dimethylallyladenine:acceptor oxidoreductase
Reaction(IUBMB)
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor [RN:R05708]
Reaction(KEGG)
Substrate
N6-dimethylallyladenine [CPD:C04083];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
adenine [CPD:C00147];
3-methylbut-2-enal [CPD:C07330];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].
Pathway
Zeatin biosynthesis
Orthology
K00279  
cytokinin dehydrogenase
Genes
ATH: 
AT1G75450(CKX5) AT2G19500(CKX2) AT2G41510(CKX1) AT4G29740(CKX4) AT5G21482(CKX7) AT5G56970(CKX3)
ALY: 
GMX: 
MTR: 
CSV: 
RCU: 
POP: 
VVI: 
OSA: 
DOSA: 
Os01t0197700-01(Os01g0197700) Os01t0775400-01(Os01g0775400) Os01t0940000-01(Os01g0940000) Os02t0220000-00(Os02g0220000) Os05t0374200-00(Os05g0374200)
BDI: 
100821104 100821608 100825123 100826954 100833368 100835621 100839711
SBI: 
SORBI_03g002810(SORBIDRAFT_03g002810) SORBI_03g003280(SORBIDRAFT_03g003280) SORBI_03g036160(SORBIDRAFT_03g036160) SORBI_03g045410(SORBIDRAFT_03g045410) SORBI_04g007730(SORBIDRAFT_04g007730) SORBI_04g007740(SORBIDRAFT_04g007740) SORBI_07g022530(SORBIDRAFT_07g022530) SORBI_09g018640(SORBIDRAFT_09g018640) SORBI_10g022590(SORBIDRAFT_10g022590)
ZMA: 
100280446(CKX10) 100526747(CKX8) 100526749(CKX5) 100526752(CKX4b) 542056(cko3) 542507(cko2) 542585(cko1)
SMO: 
SELMODRAFT_106833(CKX2-2) SELMODRAFT_174721(CKX2-1) SELMODRAFT_98722(CKX1-1)
PPP: 
PHYPADRAFT_194682 PHYPADRAFT_22106
ANA: 
all0324
AVA: 
Ava_4717
 » show all
Taxonomy
Reference
1  [PMID:11168382]
  Authors
Galuszka P, Frebort I, Sebela M, Sauer P, Jacobsen S, Pec P.
  Title
Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in cereals.
  Journal
Eur. J. Biochem. 268 (2001) 450-61.
  Organism
Arabidopsis thaliana [GN:ath]
Reference
2  [PMID:15976269]
  Authors
Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles ER, Qian Q, Kitano H, Matsuoka M
  Title
Cytokinin oxidase regulates rice grain production.
  Journal
Science. 309 (2005) 741-5.
  Organism
Oryza sativa
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
55326-39-1
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