KEGG   ENZYME: 1.7.1.14Help
Entry
EC 1.7.1.14                 Enzyme                                 
Name
nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
nitrous oxide:NAD(P) oxidoreductase
Reaction(IUBMB)
N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:R02492 R09446]
Reaction(KEGG)
Substrate
N2O [CPD:C00887];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
NO [CPD:C00533];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.
Pathway
Nitrogen metabolism
Microbial metabolism in diverse environments
Orthology
K15877  
fungal nitric oxide reductase
Genes
NCR: 
SMP: 
PAN: 
NHE: 
SSL: 
AOR: 
AOR_1_94124(AO090166000070)
ANG: 
ANI_1_2092064(An07g08930)
AFV: 
URE: 
ABE: 
TVE: 
TRV_00378
 » show all
Taxonomy
Reference
1  [PMID:2040619]
  Authors
Shoun H, Tanimoto T
  Title
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
  Journal
J. Biol. Chem. 266 (1991) 11078-82.
Reference
2  [PMID:7829493]
  Authors
Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H
  Title
Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.
  Journal
J. Biol. Chem. 270 (1995) 1617-23.
Reference
3  [PMID:12105197]
  Authors
Zhang L, Kudo T, Takaya N, Shoun H
  Title
The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.
  Journal
J. Biol. Chem. 277 (2002) 33842-7.
Reference
4  [PMID:15313618]
  Authors
Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H
  Title
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.
  Journal
J. Mol. Biol. 342 (2004) 207-17.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system