KEGG   ENZYME: 1.7.1.4Help
Entry
EC 1.7.1.4                  Enzyme                                 

Name
nitrite reductase [NAD(P)H];
nitrite reductase (reduced nicotinamide adenine dinucleotide (phosphate));
assimilatory nitrite reductase (ambiguous);
nitrite reductase [NAD(P)H2];
NAD(P)H2:nitrite oxidoreductase;
nit-6 (gene name)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
ammonia:NAD(P)+ oxidoreductase
Reaction(IUBMB)
NH3 + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H + 5 H+ [RN:R00787 R00789]
Reaction(KEGG)
Substrate
NH3 [CPD:C00014];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
nitrite [CPD:C00088];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
An iron-sulfur flavoprotein (FAD) containing siroheme. The enzymes from the fungi Neurospora crassa [1], Emericella nidulans [2] and Candida nitratophila [4] and the bacterium Aliivibrio fischeri [3] can use either NADPH or NADH as electron donor. cf. EC 1.7.1.15, nitrite reductase (NADH).
History
EC 1.7.1.4 created 1961 as EC 1.6.6.4, transferred 2002 to EC 1.7.1.4, modified 2013
Pathway
Nitrogen metabolism
Orthology
K17877  
nitrite reductase (NAD(P)H)
Genes
DHA: 
NCR: 
NCU04720(nit-6)
NTE: 
NEUTE1DRAFT87025(NEUTE1DRAFT_87025)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
VAL: 
VDA: 
ELA: 
SSL: 
BFU: 
ANI: 
AFM: 
AOR: 
AOR_1_1090194(AO090012000624)
ANG: 
ANI_1_804074(An08g05640)
AFV: 
ACT: 
NFI: 
PCS: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
DSQ: 
PCO: 
SHS: 
HIR: 
PSQ: 
GTR: 
LBC: 
MRR: 
CCI: 
SLA: 
UMA: 
PFP: 
PIF: 
PSOJ: 
 » show all
Taxonomy
Reference
1
  Authors
Nicholas, D.J.D., Medina, A. and Jones, O.T.G.
  Title
A nitrite reductase from Neurospora crassa.
  Journal
Biochim. Biophys. Acta 37 (1960) 468-476.
Reference
2  [PMID:4382427]
  Authors
Pateman JA, Rever BM, Cove DJ
  Title
Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans.
  Journal
Biochem. J. 104 (1967) 103-11.
Reference
3  [PMID:4401695]
  Authors
Prakash OM, Sadana JC
  Title
Purification, characterization and properties of nitrite reductase of Achromobacter fischeri.
  Journal
Arch. Biochem. Biophys. 148 (1972) 614-32.
Reference
4
  Authors
Rivas, J., Guerrero, M. G., Paneque, A. and Losada, M.
  Title
Characterization of the nitrate-reducing system of the yeast Torulopsis nitratophila.
  Journal
Plant Sci. Lett. 1 (1973) 105-113.
Reference
5  [PMID:4154942]
  Authors
Lafferty MA, Garrett RH
  Title
Purification and properties of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J. Biol. Chem. 249 (1974) 7555-67.
Reference
6  [PMID:126995]
  Authors
Vega JM, Garrett RH
  Title
Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J. Biol. Chem. 250 (1975) 7980-9.
Reference
7  [PMID:150863]
  Authors
Greenbaum P, Prodouz KN, Garrett RH
  Title
Preparation and some properties of homogeneous Neurospora crassa assimilatory NADPH-nitrite reductase.
  Journal
Biochim. Biophys. Acta. 526 (1978) 52-64.
Reference
8  [PMID:6457037]
  Authors
Prodouz KN, Garrett RH
  Title
Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure.
  Journal
J. Biol. Chem. 256 (1981) 9711-7.
Reference
9  [PMID:8096840]
  Authors
Exley GE, Colandene JD, Garrett RH
  Title
Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.
  Journal
J. Bacteriol. 175 (1993) 2379-92.
  Sequence
[ncr:NCU04720]
Reference
10 [PMID:8798648]
  Authors
Colandene JD, Garrett RH.
  Title
Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa.
  Journal
J. Biol. Chem. 271 (1996) 24096-104.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
9029-29-2

DBGET integrated database retrieval system