KEGG   ENZYME: 1.8.1.5Help
Entry
EC 1.8.1.5                  Enzyme                                 

Name
2-oxopropyl-CoM reductase (carboxylating);
NADPH:2-(2-ketopropylthio)ethanesulfonate oxidoreductase/carboxylase;
NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
2-mercaptoethanesulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating)
Reaction(IUBMB)
2-mercaptoethanesulfonate + acetoacetate + NADP+ = 2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH [RN:R05713]
Reaction(KEGG)
Substrate
2-mercaptoethanesulfonate [CPD:C03576];
acetoacetate [CPD:C00164];
NADP+ [CPD:C00006]
Product
2-(2-oxopropylthio)ethanesulfonate [CPD:C11497];
CO2 [CPD:C00011];
NADPH [CPD:C00005]
Comment
Also acts on thioethers longer in chain length on the oxo side, e.g. 2-oxobutyl-CoM, but this portion must be attached to CoM (2-mercaptoethanesulfonate); no CoM analogs will substitute. This enzyme forms component II of a four-component enzyme system {comprising EC 4.4.1.23 (2-hydroxypropyl-CoM lyase; component I), EC 1.8.1.5 [2-oxopropyl-CoM reductase (carboxylating); component II], EC 1.1.1.268 [2-(R)-hydroxypropyl-CoM dehydrogenase; component III] and EC 1.1.1.269 [2-(S)-hydroxypropyl-CoM dehydrogenase; component IV]} that is involved in epoxyalkane carboxylation in Xanthobacter sp. strain Py2.
History
EC 1.8.1.5 created 2001
Reference
1  [PMID:10411892]
  Authors
Allen JR, Clark DD, Krum JG, Ensign SA.
  Title
A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 96 (1999) 8432-7.
Reference
2  [PMID:10684609]
  Authors
Clark DD, Allen JR, Ensign SA.
  Title
Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system.
  Journal
Biochemistry. 39 (2000) 1294-304.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
244301-63-1

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