KEGG   ENZYME: 2.1.1.114Help
Entry
EC 2.1.1.114                Enzyme                                 

Name
polyprenyldihydroxybenzoate methyltransferase;
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase;
dihydroxyhexaprenylbenzoate methyltransferase;
COQ3 (gene name);
Coq3 O-methyltransferase;
DHHB O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:3,4-dihydroxy-5-all-trans-polyprenylbenzoate 3-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 3,4-dihydroxy-5-all-trans-polyprenylbenzoate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate [RN:R04711]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
3,4-dihydroxy-5-all-trans-polyprenylbenzoate
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate
Comment
This enzyme is involved in ubiquinone biosynthesis. Ubiquinones from different organisms have a different number of prenyl units (for example, ubiquinone-6 in Saccharomyces, ubiquinone-9 in rat and ubiquinone-10 in human), and thus the natural substrate for the enzymes from different organisms has a different number of prenyl units. However, the enzyme usually shows a low degree of specificity regarding the number of prenyl units. For example, the human COQ3 enzyme can restore biosynthesis of ubiquinone-6 in coq3 deletion mutants of yeast [3]. The enzymes from yeast and rat also catalyse the methylation of 3-demethylubiquinol-6 and 3-demethylubiquinol-9, respectively [2] (this activity is classified as EC 2.1.1.64, 3-demethylubiquinol 3-O-methyltransferase).
History
EC 2.1.1.114 created 1999
Pathway
Ubiquinone and other terpenoid-quinone biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00591  
hexaprenyldihydroxybenzoate methyltransferase
Genes
HSA: 
51805(COQ3)
PTR: 
472079(COQ3)
PPS: 
100989523(COQ3)
GGO: 
101130141(COQ3)
PON: 
NLE: 
100605053(COQ3)
MCC: 
694215(COQ3)
MCF: 
102115755(COQ3)
CJC: 
100393993(COQ3)
MMU: 
230027(Coq3)
RNO: 
29309(Coq3)
CGE: 
100763494(Coq3)
HGL: 
101715057(Coq3)
TUP: 
102497158(COQ3)
CFA: 
475004(COQ3)
AML: 
UMR: 
103675878(COQ3)
FCA: 
101084711(COQ3)
PTG: 
102957897(COQ3)
BTA: 
540298(COQ3)
BOM: 
102280603(COQ3)
PHD: 
102345018(COQ3)
CHX: 
102187404(COQ3)
OAS: 
101103985(COQ3)
SSC: 
100152047(COQ3)
CFR: 
BACU: 
102998851(COQ3)
LVE: 
103071140(COQ3)
ECB: 
100071606(COQ3)
MYB: 
102240639(COQ3)
MYD: 
102773416(COQ3)
PALE: 
102878048(COQ3)
MDO: 
100027296(COQ3)
SHR: 
100930795(COQ3)
OAA: 
100075467(COQ3)
GGA: 
421797(COQ3)
MGP: 
APLA: 
101802629(COQ3)
TGU: 
100226304(COQ3)
FAB: 
101812692(COQ3)
PHI: 
102111433(COQ3)
FPG: 
101922657(COQ3)
FCH: 
102051117(COQ3)
CLV: 
102086869(COQ3)
ASN: 
102382553(COQ3)
AMJ: 
102561582(COQ3)
PSS: 
102448168(COQ3)
CMY: 
102939536(COQ3)
ACS: 
100552641(coq3)
PBI: 
103065538(COQ3)
XLA: 
443690(coq3)
XTR: 
100486084(coq3)
DRE: 
436893(coq3)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102345501(COQ3)
CMK: 
103178294(coq3)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
412082(GB15622)
NVI: 
TCA: 
BMOR: 
API: 
100165711(Coq3)
PHU: 
ISC: 
CEL: 
CBR: 
CBG21513(Cbr-coq-3)
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
AT2G30920(COQ3)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0014s08880g(POPTRDRAFT_1098459)
SLY: 
SOT: 
OSA: 
DOSA: 
Os06t0152300-01(Os06g0152300)
OBR: 
BDI: 
SBI: 
SORBI_10g003690(SORBIDRAFT_10g003690)
ZMA: 
SITA: 
ATR: 
s00162p00043030(AMTR_s00162p00043030)
VCN: 
SCE: 
YOL096C(COQ3)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0B04170(NCAS0B04170)
NDI: 
NDAI_0G04260(NDAI0G04260)
TPF: 
TPHA_0A00670(TPHA0A00670)
TBL: 
TBLA_0B02330(TBLA0B02330)
TDL: 
TDEL_0D05250(TDEL0D05250)
KAF: 
KAFR_0C01400(KAFR0C01400)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
DDI: 
DPP: 
DFA: 
DFA_01836(coq3)
TAN: 
TPV: 
BEQ: 
CPV: 
CHO: 
TET: 
PTM: 
 » show all
Taxonomy
Reference
1  [PMID:1885593]
  Authors
Clarke CF, Williams W, Teruya JH.
  Title
Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene.
  Journal
J. Biol. Chem. 266 (1991) 16636-44.
  Sequence
[sce:YOL096C]
Reference
2  [PMID:10419476]
  Authors
Poon WW, Barkovich RJ, Hsu AY, Frankel A, Lee PT, Shepherd JN, Myles DC, Clarke CF
  Title
Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis.
  Journal
J. Biol. Chem. 274 (1999) 21665-72.
  Sequence
[sce:YOL096C]
Reference
3  [PMID:10777520]
  Authors
Jonassen T, Clarke CF
  Title
Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis.
  Journal
J. Biol. Chem. 275 (2000) 12381-7.
  Sequence
[hsa:51805]
Reference
4  [PMID:21636923]
  Authors
Xing L, Zhu Y, Fang P, Wang J, Zeng F, Li X, Teng M, Li X
  Title
Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 67 (2011) 727-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
139569-31-6

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