| Entry |
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| Name |
isoflavone 7-O-methyltransferase
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
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| Reaction(IUBMB) |
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN: R07375]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
7-hydroxyisoflavone [CPD: C15615]
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
7-methoxyisoflavone [CPD: C15616]
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| Comment |
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
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| Pathway |
| Isoflavonoid biosynthesis |
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| Orthology |
| isoflavone-7-O-methyltransferase |
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| Reference |
1 |
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| Authors |
Edwards, R. and Dixon, R.A. |
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| Title |
Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa. |
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| Journal |
Phytochemistry 30 (1991) 2597-2606. |
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| Reference |
|
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| Authors |
He XZ, Dixon RA. |
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| Title |
Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa. |
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| Journal |
Plant. Cell. 12 (2000) 1689-702. |
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| Organism |
Medicago sativa |
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| Reference |
|
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| Authors |
He XZ, Dixon RA. |
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| Title |
Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.). |
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| Journal |
Arch. Biochem. Biophys. 336 (1996) 121-9. |
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| Organism |
Medicago sativa |
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| Reference |
|
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| Authors |
He XZ, Reddy JT, Dixon RA. |
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| Title |
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase. |
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| Journal |
Plant. Mol. Biol. 36 (1998) 43-54. |
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| Organism |
Medicago sativa |
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| Reference |
|
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| Authors |
Liu CJ, Dixon RA. |
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| Title |
Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis. |
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| Journal |
Plant. Cell. 13 (2001) 2643-58. |
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| Organism |
Medicago sativa |
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| Reference |
|
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| Authors |
Zubieta C, He XZ, Dixon RA, Noel JP. |
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| Title |
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases. |
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| Journal |
Nat. Struct. Biol. 8 (2001) 271-9. |
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| Organism |
Medicago sativa |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 136111-54-1 |
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