KEGG   ENZYME: 2.1.1.150Help
Entry
EC 2.1.1.150                Enzyme                                 

Name
isoflavone 7-O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
7-hydroxyisoflavone [CPD:C15615]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
7-methoxyisoflavone [CPD:C15616]
Comment
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
Pathway
Isoflavonoid biosynthesis
Orthology
K13262  
isoflavone-7-O-methyltransferase
Reference
1
  Authors
Edwards, R. and Dixon, R.A.
  Title
Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa.
  Journal
Phytochemistry 30 (1991) 2597-2606.
Reference
2  [PMID:11006341]
  Authors
He XZ, Dixon RA.
  Title
Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa.
  Journal
Plant. Cell. 12 (2000) 1689-702.
  Organism
Medicago sativa
Reference
3  [PMID:8951042]
  Authors
He XZ, Dixon RA.
  Title
Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.).
  Journal
Arch. Biochem. Biophys. 336 (1996) 121-9.
  Organism
Medicago sativa
  Sequence
[up:O24529]
Reference
4  [PMID:9484461]
  Authors
He XZ, Reddy JT, Dixon RA.
  Title
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase.
  Journal
Plant. Mol. Biol. 36 (1998) 43-54.
  Organism
Medicago sativa
  Sequence
Reference
5  [PMID:11752378]
  Authors
Liu CJ, Dixon RA.
  Title
Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis.
  Journal
Plant. Cell. 13 (2001) 2643-58.
  Organism
Medicago sativa
Reference
6  [PMID:11224575]
  Authors
Zubieta C, He XZ, Dixon RA, Noel JP.
  Title
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases.
  Journal
Nat. Struct. Biol. 8 (2001) 271-9.
  Organism
Medicago sativa
  Sequence
[up:O24529]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
136111-54-1

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