KEGG   ENZYME: 2.1.1.180Help
Entry
EC 2.1.1.180                Enzyme                                 

Name
16S rRNA (adenine1408-N1)-methyltransferase;
kanamycin-apramycin resistance methylase;
16S rRNA:m1A1408 methyltransferase;
KamB;
NpmA;
16S rRNA m1A1408 methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:16S rRNA (adenine1408-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + adenine1408 in 16S rRNA = S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
adenine1408 in 16S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methyladenine1408 in 16S rRNA
Comment
The enzyme provides a panaminoglycoside-resistant nature through interference with the binding of aminoglycosides toward the A site of 16S rRNA through N1-methylation at position adenine1408 [4].
History
EC 2.1.1.180 created 2010
Orthology
K18846  
16S rRNA (adenine(1408)-N(1))-methyltransferase
Genes
SCL: 
SCU: 
ASF: 
ASM: 
ASO: 
ASB: 
TCU: 
CAI: 
WWE: 
Taxonomy
Reference
1  [PMID:2441068]
  Authors
Beauclerk AA, Cundliffe E
  Title
Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides.
  Journal
J. Mol. Biol. 193 (1987) 661-71.
Reference
2  [PMID:17495534]
  Authors
Koscinski L, Feder M, Bujnicki JM
  Title
Identification of a missing sequence and functionally important residues of 16S rRNA:m(1)A1408 methyltransferase KamB that causes bacterial resistance to aminoglycoside antibiotics.
  Journal
Cell. Cycle. 6 (2007) 1268-71.
Reference
3  [PMID:1840536]
  Authors
Holmes DJ, Drocourt D, Tiraby G, Cundliffe E
  Title
Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius.
  Journal
Gene. 102 (1991) 19-26.
Reference
4  [PMID:17875999]
  Authors
Wachino J, Shibayama K, Kurokawa H, Kimura K, Yamane K, Suzuki S, Shibata N, Ike Y, Arakawa Y
  Title
Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides.
  Journal
Antimicrob. Agents. Chemother. 51 (2007) 4401-9.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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