| Entry |
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| Name |
23S rRNA (guanine748-N1)-methyltransferase;
Rlma(II);
Rlma2;
23S rRNA m1G748 methyltransferase;
RlmaII;
Rlma II;
tylosin-resistance methyltransferase RlmA(II);
TlrB;
rRNA large subunit methyltransferase II
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase
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| Reaction(IUBMB) |
S-adenosyl-L-methionine + guanine748 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA [RN: R07233]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
guanine748 in 23S rRNA
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
N1-methylguanine748 in 23S rRNA
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| Comment |
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].
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| Orthology |
| 23S rRNA (guanine748-N1)-methyltransferase |
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| Reference |
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| Authors |
Douthwaite S, Crain PF, Liu M, Poehlsgaard J |
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| Title |
The tylosin-resistance methyltransferase RlmA(II) (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748. |
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| Journal |
J. Mol. Biol. 337 (2004) 1073-7. |
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| Reference |
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| Authors |
Liu M, Kirpekar F, Van Wezel GP, Douthwaite S |
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| Title |
The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA. |
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| Journal |
Mol. Microbiol. 37 (2000) 811-20. |
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| Reference |
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| Authors |
Lebars I, Yoshizawa S, Stenholm AR, Guittet E, Douthwaite S, Fourmy D |
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| Title |
Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII. |
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| Journal |
EMBO. J. 22 (2003) 183-92. |
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| Reference |
|
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| Authors |
Lebars I, Husson C, Yoshizawa S, Douthwaite S, Fourmy D |
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| Title |
Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II). |
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| Journal |
J. Mol. Biol. 372 (2007) 525-34. |
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| Reference |
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| Authors |
Douthwaite S, Jakobsen L, Yoshizawa S, Fourmy D |
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| Title |
Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I. |
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| Journal |
J. Mol. Biol. 378 (2008) 969-75. |
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| Reference |
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| Authors |
Liu M, Douthwaite S |
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| Title |
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria. |
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| Journal |
Mol. Microbiol. 44 (2002) 195-204. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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