KEGG   ENZYME: 2.1.1.202Help
Entry
EC 2.1.1.202                Enzyme                                 

Name
multisite-specific tRNA:(cytosine-C5)-methyltransferase;
multisite-specific tRNA:m5C-methyltransferase;
TRM4 (gene name, gene corresponding to ORF YBL024w)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor [RN:R00595];
(2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor;
(3) S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA;
(4) S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cytosine34 in tRNA precursor;
cytosine40 in tRNA precursor;
cytosine48 in tRNA;
cytosine49 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
5-methylcytosine34 in tRNA precursor;
5-methylcytosine40 in tRNA precursor;
5-methylcytosine48 in tRNA;
5-methylcytosine49 in tRNA
Comment
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA. The incidence of modification depends on the cytosine position in tRNA. At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNALeu(CUA) and tRNAPhe(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine48 or 5-methylcytosine49, but never both in the same tRNA molecule [1]. The formation of 5-methylcytosine34 and 5-methylcytosine40 is a strictly intron-dependent process, whereas the formation of 5-methylcytosine48 and 5-methylcytosine49 is an intron-independent process [2,3].
History
EC 2.1.1.202 created 1976 as EC 2.1.1.29, part transferred 2011 to EC 2.1.1.202
Orthology
K15334  
multisite-specific tRNA:(cytosine-C5)-methyltransferase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os08t0484400-01(Os08g0484400) Os09t0471900-01(Os09g0471900)
OBR: 
BDI: 
SBI: 
SORBI_02g027340(SORBIDRAFT_02g027340)
ZMA: 
SITA: 
PDA: 
MUS: 
ATR: 
s00008p00028880(AMTR_s00008p00028880)
SMO: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CVR: 
SCE: 
YBL024W(NCL1)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0B05640(NCAS0B05640)
NDI: 
NDAI_0B01830(NDAI0B01830) NDAI_0B02980(NDAI0B02980)
TPF: 
TPHA_0C02870(TPHA0C02870) TPHA_0H00150(TPHA0H00150)
TBL: 
TBLA_0A08740(TBLA0A08740)
TDL: 
TDEL_0B04390(TDEL0B04390)
KAF: 
KAFR_0B02220(KAFR0B02220)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.518(NCL1) CaO19.8149(NCL1)
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
ELA: 
SSL: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_768194(AO090012000444)
ANG: 
ANI_1_1640014(An01g12180)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
TMS: 
DSQ: 
PCO: 
SHS: 
PSQ: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
CPUT: 
SLA: 
UMA: 
PFP: 
MGL: 
MLR: 
WSE: 
 » show all
Taxonomy
Reference
1  [PMID:10445884]
  Authors
Motorin Y, Grosjean H
  Title
Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.
  Journal
RNA. 5 (1999) 1105-18.
  Sequence
[sce:YBL024W]
Reference
2  [PMID:9207014]
  Authors
Jiang HQ, Motorin Y, Jin YX, Grosjean H
  Title
Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study.
  Journal
Nucleic. Acids. Res. 25 (1997) 2694-701.
Reference
3  [PMID:3537724]
  Authors
Strobel MC, Abelson J
  Title
Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.
  Journal
Mol. Cell. Biol. 6 (1986) 2663-73.
Reference
4  [PMID:17475914]
  Authors
Walbott H, Husson C, Auxilien S, Golinelli-Pimpaneau B
  Title
Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase.
  Journal
RNA. 13 (2007) 967-73.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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