KEGG   ENZYME: 2.1.1.203Help
Entry
EC 2.1.1.203                Enzyme                                 

Name
tRNA (cytosine34-C5)-methyltransferase;
hTrm4 Mtase;
hTrm4 methyltransferase;
hTrm4 (gene name);
tRNA:m5C-methyltransferase (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytosine34-C5)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor [RN:R00595]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cytosine34 in tRNA precursor
Product
S-adenosyl-L-homocysteine [CPD:C00021];
5-methylcytosine34 in tRNA precursor
Comment
The human enzyme is specific for C5-methylation of cytosine34 in tRNA precursors. The intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. It is not able to form 5-methylcytosine at positions 48 and 49 of human and yeast tRNA precursors [1].
History
EC 2.1.1.203 created 1976 as EC 2.1.1.29, part transferred 2011 to EC 2.1.1.203
Orthology
K15335  
tRNA (cytosine34-C5)-methyltransferase
Genes
HSA: 
54888(NSUN2)
PTR: 
461711(NSUN2)
PPS: 
100978191(NSUN2)
GGO: 
101144331(NSUN2)
PON: 
100443369(NSUN2)
NLE: 
100606775(NSUN2)
MCC: 
694987(NSUN2)
MCF: 
102125748(NSUN2)
CSAB: 
103214972(NSUN2)
RRO: 
104672269(NSUN2)
RBB: 
108522883(NSUN2)
CJC: 
100391414(NSUN2)
SBQ: 
101044631(NSUN2)
MMU: 
28114(Nsun2)
RNO: 
361191(Nsun2)
CGE: 
100760286(Nsun2)
NGI: 
103735347(Nsun2)
HGL: 
101714363(Nsun2)
CCAN: 
109693822(Nsun2)
OCU: 
100350862(NSUN2)
TUP: 
102500776(NSUN2)
CFA: 
478625(NSUN2)
AML: 
100472688(NSUN2)
UMR: 
103679252(NSUN2)
ORO: 
101363016(NSUN2)
FCA: 
101099103(NSUN2)
PTG: 
102952778(NSUN2)
AJU: 
106971247(NSUN2)
BTA: 
516044(NSUN2)
BOM: 
102272859(NSUN2)
BIU: 
109574872(NSUN2)
PHD: 
102327918(NSUN2)
CHX: 
102180428(NSUN2)
OAS: 
101106968(NSUN2)
SSC: 
100518405(NSUN2)
CFR: 
102513890(NSUN2)
CDK: 
105094036(NSUN2)
BACU: 
102998396(NSUN2)
LVE: 
103071447(NSUN2)
OOR: 
101275527(NSUN2)
ECB: 
100071458(NSUN2)
EPZ: 
103550089(NSUN2)
EAI: 
106822986(NSUN2)
MYB: 
102260943(NSUN2)
MYD: 
102762450(NSUN2)
HAI: 
109376003(NSUN2)
RSS: 
109453573(NSUN2)
PALE: 
102893514(NSUN2)
LAV: 
100673463(NSUN2)
TMU: 
101347263
MDO: 
100018054(NSUN2)
SHR: 
100920695(NSUN2)
OAA: 
100092642(NSUN2)
GGA: 
420938(NSUN2)
MGP: 
100541040(NSUN2)
CJO: 
107309500(NSUN2)
APLA: 
101792480(NSUN2)
ACYG: 
106036579(NSUN2)
TGU: 
100223811(NSUN2)
GFR: 
102039201(NSUN2)
FAB: 
101814592(NSUN2)
PHI: 
102110302(NSUN2)
PMAJ: 
107200160(NSUN2)
CCW: 
104685923(NSUN2)
FPG: 
101910766(NSUN2)
FCH: 
102049337(NSUN2)
CLV: 
102086935(NSUN2)
EGZ: 
104126189(NSUN2)
AAM: 
106492334(NSUN2)
ASN: 
102380740(NSUN2)
AMJ: 
102565534(NSUN2)
PSS: 
102452913(NSUN2)
CMY: 
102940691(NSUN2)
CPIC: 
101953716(NSUN2)
ACS: 
100555854(nsun2)
PVT: 
110080022(NSUN2)
PBI: 
103053000 103065582(NSUN2)
GJA: 
107123742(NSUN2)
XLA: 
414460(nsun2.S)
XTR: 
548716(nsun2)
NPR: 
108795130(NSUN2)
DRE: 
325292(nsun2)
SRX: 
107711338 107754797(nsun2)
SANH: 
107691499 107694333
SGH: 
107584336(nsun2) 107595605 107595606
CCAR: 
109110846
IPU: 
108273268(nsun2)
AMEX: 
103038375(nsun2)
TRU: 
101074500(nsun2)
TNG: 
GSTEN00017585G001
LCO: 
104922766(nsun2)
NCC: 
104959827 104961211(nsun2)
MZE: 
101476280(nsun2)
OLA: 
101158463(nsun2)
XMA: 
102222973(nsun2)
PRET: 
103472067(nsun2)
NFU: 
107383181(nsun2)
CSEM: 
103394106(nsun2)
LCF: 
108900394(nsun2)
HCQ: 
109519206(nsun2)
BPEC: 
110167265(nsun2)
SASA: 
106570340(nsun2) 106588694
ELS: 
105012406(nsun2)
SFM: 
108919300(nsun2)
LCM: 
102346516 102349429 102352869(NSUN2)
CMK: 
103183195(nsun2)
BFO: 
BRAFLDRAFT_118815
CIN: 
100184803
SPU: 
592875
APLC: 
110989010
SKO: 
102802422 102803327
DME: 
Dmel_CG6133(Nsun2)
DPO: 
Dpse_GA19379
DAN: 
Dana_GF21493
DER: 
Dere_GG18560(Dere_EG:EG0007.9)
DPE: 
Dper_GL21386
DSE: 
Dsec_GM12703
DSI: 
Dsimw501_GD16312(Dsim_EG:EG0007.9)
DWI: 
Dwil_GK17644
DYA: 
Dyak_GE16872(Dyak_EG:EG0007.9)
DGR: 
Dgri_GH24818
DMO: 
Dmoj_GI16150
DVI: 
Dvir_GJ16427
MDE: 
101892384
AGA: 
AgaP_AGAP002504
AAG: 
5579023
CQU: 
CpipJ_CPIJ010001
AME: 
411579
BIM: 
100740667
BTER: 
100650195
SOC: 
105203136
AEC: 
105149871
ACEP: 
105621541
PBAR: 
105432310
HST: 
105182995
CFO: 
105258017 105258037
LHU: 
105668308
PGC: 
109855240
NVI: 
100116182
TCA: 
662306
DPA: 
109535099
NVL: 
108557299
BMOR: 
101745118
DPL: 
KGM_212221
PMAC: 
106707772 106711911 106720529
PRAP: 
110999001
PXY: 
105380786
API: 
100169525
DNX: 
107167504
PHU: 
Phum_PHUM474660
ZNE: 
110829145
FCD: 
110858480
DPX: 
DAPPUDRAFT_329423
ISC: 
IscW_ISCW003251
TUT: 
107368159
CEL: 
CELE_Y48G8AL.5(Y48G8AL.5)
CBR: 
CBG08493
LOA: 
LOAG_16801
TSP: 
Tsp_00405
HRO: 
HELRODRAFT_68497
LGI: 
LOTGIDRAFT_122705 LOTGIDRAFT_176410
CRG: 
105343718
MYI: 
110446873
OBI: 
106883416
LAK: 
106159806 106177539
SMM: 
Smp_175950
SHX: 
MS3_09900
OVI: 
T265_05975
NVE: 
NEMVE_v1g20689
EPA: 
110245842
ADF: 
107338283
HMG: 
100214074
TAD: 
TRIADDRAFT_19981
AQU: 
100636553 100637475 109590444
CRE: 
CHLREDRAFT_114222(TRM4)
OLU: 
OSTLU_16406
OTA: 
OT_ostta08g01420 OT_ostta14g02040
BPG: 
Bathy14g01140
MIS: 
MICPUN_66831 MICPUN_79216
MPP: 
MICPUCDRAFT_15736 MICPUCDRAFT_59415
CSL: 
COCSUDRAFT_15359
CME: 
CYME_CMG165C
GSL: 
Gasu_11160
ECU: 
ECU08_0400
EIN: 
Eint_080320
EHE: 
EHEL_080320
ERO: 
EROM_080320
NCE: 
NCER_101435 NCER_101436
MBR: 
MONBRDRAFT_36363 MONBRDRAFT_38306
SRE: 
PTSG_02162
DDI: 
DDB_G0295715
DPP: 
DICPUDRAFT_30335
DFA: 
DFA_07613
EIV: 
EIN_184050
ACAN: 
ACA1_071630 ACA1_189790 ACA1_189800
TAN: 
TA19955
TOT: 
TOT_010000071
BEQ: 
BEWA_033980
BBO: 
BBOV_IV000560(21.m02754)
CPV: 
cgd7_1630
TET: 
TTHERM_00028590 TTHERM_00684540
SMIN: 
v1.2.013460.t1(symbB.v1.2.013460.t1) v1.2.033994.t1(symbB.v1.2.033994.t1) v1.2.037264.t1(symbB.v1.2.037264.t1)
PTI: 
PHATRDRAFT_48154
FCY: 
FRACYDRAFT_189551
TPS: 
THAPSDRAFT_23686 THAPSDRAFT_24350 THAPSDRAFT_39812
AAF: 
AURANDRAFT_68423
PIF: 
PITG_00448 PITG_02560 PITG_17253 PITG_17298
PSOJ: 
PHYSODRAFT_248298 PHYSODRAFT_447412 PHYSODRAFT_477678 PHYSODRAFT_551899
SPAR: 
SPRG_00868 SPRG_04176 SPRG_05360 SPRG_20720 SPRG_21894
EHX: 
EMIHUDRAFT_194550 EMIHUDRAFT_452763 EMIHUDRAFT_459528 EMIHUDRAFT_96831
GTT: 
GUITHDRAFT_46333 GUITHDRAFT_55503 GUITHDRAFT_62957 GUITHDRAFT_82201 GUITHDRAFT_99887
TBR: 
Tb10.70.0080 Tb927.3.3230(Tb03.3K10.270)
TCR: 
506699.3 508153.370 510187.240
LMA: 
LMJF_29_2540 LMJF_36_2170
LIF: 
LINJ_29_2650 LINJ_36_2300
LDO: 
LDBPK_292650 LDBPK_362300
LMI: 
LMXM_08_29_2540 LMXM_36_2170
LBZ: 
LBRM_29_2510 LBRM_35_2410
NGR: 
NAEGRDRAFT_56581 NAEGRDRAFT_80707
TVA: 
TVAG_079770 TVAG_427320
GLA: 
GL50803_17199
 » show all
Taxonomy
Reference
1  [PMID:17071714]
  Authors
Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J, Szweykowska-Kulinska Z
  Title
Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA).
  Journal
Nucleic. Acids. Res. 34 (2006) 6034-43.
  Sequence
[hsa:54888]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system