KEGG   ENZYME: 2.1.1.203Help
Entry
EC 2.1.1.203                Enzyme                                 
Name
tRNA (cytosine34-C5)-methyltransferase;
hTrm4 Mtase;
hTrm4 methyltransferase;
hTrm4 (gene name);
tRNA:m5C-methyltransferase (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytosine34-C5)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor [RN:R00595]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cytosine34 in tRNA precursor
Product
S-adenosyl-L-homocysteine [CPD:C00021];
5-methylcytosine34 in tRNA precursor
Comment
The human enzyme is specific for C5-methylation of cytosine34 in tRNA precursors. The intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. It is not able to form 5-methylcytosine at positions 48 and 49 of human and yeast tRNA precursors [1].
Orthology
K15335  
tRNA (cytosine34-C5)-methyltransferase
Genes
HSA: 
54888(NSUN2)
PTR: 
461711(NSUN2)
PPS: 
100978191(NSUN2)
GGO: 
101144331(NSUN2)
PON: 
100443369(NSUN2)
MCC: 
694987(NSUN2)
MCF: 
102125748(NSUN2)
MMU: 
28114(Nsun2)
RNO: 
361191(Nsun2)
CGE: 
100760286(Nsun2)
HGL: 
101714363(Nsun2)
TUP: 
102500776(NSUN2)
CFA: 
478625(NSUN2)
AML: 
100472688(NSUN2)
FCA: 
101099103(NSUN2)
BTA: 
516044(NSUN2)
BOM: 
102272859(NSUN2)
PHD: 
102327918(NSUN2)
CHX: 
102180428(NSUN2)
SSC: 
100518405(NSUN2)
CFR: 
102513890(NSUN2)
ECB: 
100071458(NSUN2)
MYB: 
102260943(NSUN2)
MDO: 
100018054(NSUN2)
SHR: 
100920695(NSUN2)
OAA: 
100092642(NSUN2)
GGA: 
420938(NSUN2)
MGP: 
100541040
TGU: 
100223811(NSUN2)
FAB: 
101814592(NSUN2)
PHI: 
102110302(NSUN2)
APLA: 
101792480(NSUN2)
FPG: 
101910766(NSUN2)
FCH: 
102049337(NSUN2)
CLV: 
102086935(NSUN2)
ASN: 
102380740(NSUN2)
PSS: 
102452913(NSUN2)
ACS: 
100555854
XLA: 
414460(nsun2)
XTR: 
548716(nsun2)
DRE: 
325292(nsun2)
TRU: 
101074500
MZE: 
101476280
OLA: 
101158463
XMA: 
102222973
BFO: 
BRAFLDRAFT_118815
CIN: 
100184803
SPU: 
592875
DME: 
Dmel_CG6133(Nsun2)
DPO: 
Dpse_GA19379
DAN: 
Dana_GF21493
DER: 
Dere_GG18560(Dere_EG:EG0007.9)
DPE: 
Dper_GL21386
DSE: 
Dsec_GM12703
DSI: 
Dsim_GD16312(Dsim_EG:EG0007.9)
DWI: 
Dwil_GK17644
DYA: 
Dyak_GE16872(Dyak_EG:EG0007.9)
DGR: 
Dgri_GH24818
DMO: 
Dmoj_GI16150
DVI: 
Dvir_GJ16427
AGA: 
AgaP_AGAP002504
AAG: 
AaeL_AAEL013968
CQU: 
CpipJ_CPIJ010001
AME: 
411579(GB12100)
NVI: 
100116182
TCA: 
662306
BMOR: 
101745118
API: 
100169525
PHU: 
Phum_PHUM474660
ISC: 
IscW_ISCW003251
CEL: 
CELE_Y48G8AL.5(Y48G8AL.5)
CBR: 
CBG08493
SMM: 
Smp_175950
NVE: 
NEMVE_v1g20689
HMG: 
100214074
TAD: 
TRIADDRAFT_19981
AQU: 
100637475
SBI: 
SORBI_07g029085(SORBIDRAFT_07g029085)
OLU: 
OSTLU_16406
OTA: 
Ot04g00960 Ot14g02380
CME: 
CYME_CMG165C
ECU: 
ECU08_0400
MBR: 
MONBRDRAFT_36363 MONBRDRAFT_38306
DDI: 
DDB_G0295715
TAN: 
TA19955
BBO: 
BBOV_IV000560(21.m02754)
CPV: 
cgd7_1630
TGO: 
TGME49_094440
TET: 
TTHERM_00028590 TTHERM_00684540
PTI: 
PHATRDRAFT_48154
TPS: 
THAPSDRAFT_23686 THAPSDRAFT_24350 THAPSDRAFT_39812
PIF: 
PITG_00448 PITG_02560 PITG_17253 PITG_17298
TCR: 
506699.3 508153.370 510187.240
LMA: 
LMJF_29_2540 LMJF_36_2170
LIF: 
LINJ_29_2650 LINJ_36_2300
NGR: 
NAEGRDRAFT_56581
TVA: 
TVAG_079770 TVAG_427320
GLA: 
GL50803_17199
 » show all
Taxonomy
Reference
1  [PMID:17071714]
  Authors
Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J, Szweykowska-Kulinska Z
  Title
Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA).
  Journal
Nucleic. Acids. Res. 34 (2006) 6034-43.
  Organism
Homo sapiens [GN:hsa]
  Sequence
[hsa:54888]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system