KEGG   ENZYME: 2.1.1.244Help
Entry
EC 2.1.1.244                Enzyme                                 

Name
protein N-terminal methyltransferase;
NMT1 (gene name);
METTL11A (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-[protein] methyltransferase
Reaction(IUBMB)
(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction);
(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein];
(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
(2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction);
(2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein];
(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]
Substrate
S-adenosyl-L-methionine [CPD:C00019];
N-terminal-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein];
N-terminal-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Comment
This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. When the terminal amino acid is L-proline, the enzyme catalyses two successive methylations of its alpha-amino group. When the first amino acid is either L-alanine or L-serine, the enzyme catalyses three successive methylations.
The Pro-Lys in positions 2-3 cannot be exchanged for other amino acids [1,2].
History
EC 2.1.1.244 created 2012
Orthology
K16219  
protein N-terminal methyltransferase
Genes
HSA: 
28989(NTMT1)
PTR: 
464790(NTMT1)
PPS: 
100987374(NTMT1)
GGO: 
101142309(NTMT1)
PON: 
100431666(NTMT1)
NLE: 
100603188(NTMT1)
MCC: 
716763(NTMT1)
MCF: 
102126169(NTMT1)
RRO: 
104660538(NTMT1)
CJC: 
100415418(NTMT1)
MMU: 
66617(Ntmt1)
RNO: 
362103(Ntmt1)
CGE: 
100771449(Ntmt1)
NGI: 
103727878(Ntmt1)
HGL: 
101704679(Ntmt1)
OCU: 
100339797(NTMT1)
TUP: 
102484497(NTMT1)
CFA: 
491300(NTMT1)
AML: 
100464977(NTMT1)
UMR: 
103670267(NTMT1)
FCA: 
101085326(NTMT1)
PTG: 
102970588(NTMT1)
BTA: 
617042(NTMT1)
BOM: 
102266812(NTMT1)
PHD: 
102332242(NTMT1)
CHX: 
102172454(NTMT1)
OAS: 
101115521(NTMT1)
SSC: 
100153919(NTMT1)
CFR: 
102516861(NTMT1)
BACU: 
103004017(NTMT1)
LVE: 
103077988(NTMT1)
ECB: 
100066820(NTMT1)
MYB: 
MYD: 
102765040(NTMT1)
PALE: 
102893601(NTMT1)
MDO: 
100012728(NTMT1)
SHR: 
GGA: 
425539(NTMT1)
MGP: 
CJO: 
107321904(NTMT1)
APLA: 
101791577(NTMT1)
TGU: 
100222445(NTMT1)
GFR: 
FAB: 
101813844(NTMT1)
PHI: 
102112016(NTMT1)
CCW: 
104688292(NTMT1)
FPG: 
101914918(NTMT1)
FCH: 
102054150(NTMT1)
CLV: 
102084809(NTMT1)
AAM: 
ASN: 
102385028(NTMT1)
AMJ: 
102572961(NTMT1)
PSS: 
102449305(NTMT1)
CMY: 
102947090(NTMT1)
PBI: 
103065843(NTMT1)
GJA: 
107109083(NTMT1)
XLA: 
734671(ntmt1) 735155(ntmt1)
XTR: 
100145456(ntmt1)
DRE: 
405834(ntmt1)
TRU: 
101071402(ntmt1)
MZE: 
101485931(ntmt1)
OLA: 
101168668(ntmt1)
XMA: 
102223153(ntmt1)
LCM: 
102358139(NTMT1)
CMK: 
103184690(ntmt1)
BFO: 
CIN: 
SPU: 
SKO: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
Dsimw501_GD10692(Dsim_GD10692)
DWI: 
DYA: 
Dyak_GE19310(dyak_GLEANR_3083)
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
AME: 
550780(GB11662)
SOC: 
AEC: 
HST: 
CFO: 
NVI: 
TCA: 
BMOR: 
PXY: 
API: 
100167499(Mettl11a)
PHU: 
ISC: 
CEL: 
CBR: 
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
CRG: 
OBI: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0011s16080g(POPTRDRAFT_569328)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os03t0780900-01(Os03g0780900)
OBR: 
BDI: 
SBI: 
SORBI_01g006290(SORBIDRAFT_01g006290)
ZMA: 
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
APRO: 
GSL: 
SCE: 
YBR261C(TAE1)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0B07330(NCAS0B07330)
NDI: 
NDAI_0B04650(NDAI0B04650)
TPF: 
TPHA_0O01360(TPHA0O01360)
TBL: 
TBLA_0F03410(TBLA0F03410)
TDL: 
TDEL_0D02090(TDEL0D02090)
KAF: 
KAFR_0C04340(KAFR0C04340)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
NTE: 
NEUTE1DRAFT76613(NEUTE1DRAFT_76613)
SMP: 
PAN: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VDA: 
ELA: 
SSL: 
MBE: 
AFM: 
AOR: 
AOR_1_388134(AO090102000220)
ANG: 
ANI_1_940134(An15g06850)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
PBN: 
ABE: 
TVE: 
AJE: 
PNO: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNB: 
TMS: 
DSQ: 
SHS: 
HIR: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT35058(AGABI1DRAFT_35058)
ABV: 
AGABI2DRAFT148576(AGABI2DRAFT_148576)
SLA: 
WSE: 
UMA: 
PFP: 
MGL: 
MLR: 
MBR: 
SRE: 
DDI: 
DPP: 
DFA: 
EHI: 
EHI_036850(283.t00004)
EDI: 
EIV: 
ACAN: 
TGO: 
TET: 
PTM: 
PTI: 
TPS: 
PIF: 
PSOJ: 
SPAR: 
EHX: 
GTT: 
TBR: 
Tb927.6.2270(Tb06.4M18.660) Tb927.6.2330(Tb06.4M18.740)
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
TVA: 
GLA: 
 » show all
Taxonomy
Reference
1  [PMID:20481588]
  Authors
Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG
  Title
Identification of protein N-terminal methyltransferases in yeast and humans.
  Journal
Biochemistry. 49 (2010) 5225-35.
  Sequence
[hsa:28989] [sce:YBR261C]
Reference
2  [PMID:20668449]
  Authors
Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG
  Title
NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.
  Journal
Nature. 466 (2010) 1125-8.
  Sequence
[hsa:28989] [mmu:66617]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system