KEGG   ENZYME: 2.1.1.249Help
Entry
EC 2.1.1.249                Enzyme                                 

Name
dimethylamine---corrinoid protein Co-methyltransferase;
mtbB (gene name);
dimethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine [RN:R10015]
Reaction(KEGG)
R10015;
(other) R09999
Show
Substrate
dimethylamine [CPD:C00543];
[Co(I) dimethylamine-specific corrinoid protein]
Product
[methyl-Co(III) dimethylamine-specific corrinoid protein];
methylamine [CPD:C00218]
Comment
The enzyme, which catalyses the transfer of a methyl group from dimethylamine to a dimethylamine-specific corrinoid protein (MtbC), is involved in methanogenesis from dimethylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.249 created 2012
Pathway
Methane metabolism
Microbial metabolism in diverse environments
Orthology
K16178  
dimethylamine---corrinoid protein Co-methyltransferase
Genes
TPZ: 
Tph_c27710(mtbB1) Tph_c27720(mtbB2)
AAR: 
MAC: 
MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)
MBY: 
MBW: 
MBAR: 
MMA: 
MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)
MMAZ: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MMET: 
MEV: 
MZH: 
MPY: 
Mpsy_1680(mtbB) Mpsy_1701(mtbB2) Mpsy_1702(mtbB3)
MHZ: 
TAR: 
MAX: 
MER: 
MARC: 
 » show all
Taxonomy
Reference
1  [PMID:9654067]
  Authors
Wassenaar RW, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro.
  Journal
Eur. J. Biochem. 253 (1998) 692-7.
Reference
2  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J. Biol. Chem. 275 (2000) 29053-60.
  Sequence
Reference
3  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr. Opin. Chem. Biol. 8 (2004) 484-91.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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