KEGG   ENZYME: 2.1.1.254Help
Entry
EC 2.1.1.254                Enzyme                                 

Name
erythromycin 3''-O-methyltransferase;
EryG
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + erythromycin C = S-adenosyl-L-homocysteine + erythromycin A [RN:R05520];
(2) S-adenosyl-L-methionine + erythromycin D = S-adenosyl-L-homocysteine + erythromycin B [RN:R05532]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
erythromycin C [CPD:C06616];
erythromycin D [CPD:C06633]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
erythromycin A [CPD:C01912];
erythromycin B [CPD:C06653]
Comment
The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC 1.14.13.154, erythromycin 12 hydroxylase.
Pathway
Biosynthesis of 12-, 14- and 16-membered macrolides
Biosynthesis of secondary metabolites
Orthology
K14369  
erythromycin 3''-O-methyltransferase
Genes
SEN: 
SACE_0728(eryG)
Taxonomy
Reference
1  [PMID:2185226]
  Authors
Paulus TJ, Tuan JS, Luebke VE, Maine GT, DeWitt JP, Katz L
  Title
Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli.
  Journal
J. Bacteriol. 172 (1990) 2541-6.
  Organism
Saccharopolyspora erythraea [GN:sen]
  Sequence
[sen:SACE_0728]
Reference
2  [PMID:9353926]
  Authors
Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L.
  Title
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
  Journal
Microbiology. 143 ( Pt 10) (1997) 3251-62.
  Organism
Saccharopolyspora erythraea [GN:sen]
  Sequence
[sen:SACE_0728]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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