KEGG   ENZYME: 2.1.1.260Help
Entry
EC 2.1.1.260                Enzyme                                 

Name
rRNA small subunit pseudouridine methyltransferase Nep1;
Nep1;
nucleolar essential protein 1
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:18S rRNA (pseudouridine1191-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA = S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
pseudouridine1191 in yeast 18S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylpseudouridine1191 in yeast 18S rRNA
Comment
This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. It recognizes Psi914 in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi1191 in yeast 18S rRNA, and Psi1248 in human 18S rRNA.
History
EC 2.1.1.260 created 2012
Orthology
K14568  rRNA small subunit pseudouridine methyltransferase Nep1
Genes
HSA: 10436(EMG1)
PTR: 451806(EMG1)
PPS: 100993485(EMG1)
GGO: 101139632(EMG1)
PON: 100450148(EMG1)
NLE: 100598500(EMG1)
MCC: 722122(EMG1)
MCF: 102135921(EMG1)
CSAB: 103218495(EMG1)
RRO: 104680082(EMG1)
RBB: 108542028(EMG1)
CJC: 100411554(EMG1)
SBQ: 101036212(EMG1)
MMU: 14791(Emg1)
RNO: 312706(Emg1)
CGE: 100759485(Emg1)
NGI: 103727489(Emg1)
HGL: 101726764(Emg1)
CCAN: 109682816(Emg1)
OCU: 100342038(EMG1)
TUP: 102497301(EMG1)
CFA: 477708(EMG1)
AML: 100473024(EMG1)
UMR: 103656178(EMG1)
ORO: 101375618(EMG1)
FCA: 101088173(EMG1)
PTG: 102949816(EMG1)
AJU: 106965340(EMG1)
BTA: 515362(EMG1)
BOM: 102285025(EMG1)
BIU: 109558905(EMG1)
PHD: 102326240(EMG1)
CHX: 102189562(EMG1)
OAS: 101108377(EMG1)
SSC: 100627558(EMG1)
CFR: 102513682(EMG1)
CDK: 105098190(EMG1)
BACU: 103004463(EMG1)
LVE: 103086149(EMG1)
OOR: 101290483(EMG1)
ECB: 100052984(EMG1)
EPZ: 103547029(EMG1)
EAI: 106847312(EMG1)
MYB: 102255915(EMG1) 102259485
MYD: 102754127(EMG1)
HAI: 109373661(EMG1)
RSS: 109459686(EMG1)
PALE: 102896616(EMG1)
LAV: 100677554(EMG1)
TMU: 101358623
MDO: 100014133(EMG1)
SHR: 100916926(EMG1)
GGA: 418292(EMG1)
MGP: 100549249(EMG1)
CJO: 107306763 107318472(EMG1)
APLA: 101795903(EMG1)
ACYG: 106047841(EMG1)
TGU: 100223062(EMG1)
GFR: 102042844(EMG1)
FAB: 101812607(EMG1)
PHI: 102109724(EMG1)
PMAJ: 107207111(EMG1)
FPG: 101914863(EMG1)
FCH: 102054948(EMG1)
CLV: 102093025(EMG1)
EGZ: 104126542(EMG1)
AAM: 106497214(EMG1)
ASN: 102376748(EMG1)
AMJ: 102572198(EMG1)
PSS: 102450259(EMG1)
CMY: 102932916(EMG1)
CPIC: 101943638(EMG1)
PVT: 110084642(EMG1)
PBI: 103066098(EMG1)
GJA: 107116806(EMG1)
XLA: 108696043(emg1.L) 108697334(emg1.S)
XTR: 100485133(emg1)
NPR: 108784386(EMG1)
DRE: 678588(emg1)
SGH: 107569993(emg1)
CCAR: 109105743(emg1)
IPU: 108268384(emg1)
AMEX: 103028869(emg1)
TRU: 101066424(emg1)
LCO: 104929009(emg1)
NCC: 104957146(emg1)
MZE: 101473610(emg1)
OLA: 101172018(emg1)
XMA: 102217413(emg1)
PRET: 103478015(emg1)
NFU: 107379442(emg1)
CSEM: 103387936(emg1)
LCF: 108887348(emg1)
HCQ: 109515374(emg1)
BPEC: 110163013(emg1)
SASA: 106575899 106576677(emg1)
ELS: 105019061(emg1)
SFM: 108925023(emg1)
LCM: 102355452(EMG1)
CMK: 103175877(emg1)
CIN: 100184403
SPU: 589275
APLC: 110985399
SKO: 100378866
DSI: Dsimw501_GD16696(Dsim_GD16696)
MDE: 101895994
AAG: 5573604
AME: 409857
BIM: 100744183
BTER: 100650006
SOC: 105201670
PBAR: 105432227
HST: 105186160
CFO: 105250085
LHU: 105668151
PGC: 109854455
NVI: 100680224
TCA: 664400
DPA: 109539687
NVL: 108566853
BMOR: 100862801(Nep1)
PMAC: 106719614
PRAP: 111004291
PXY: 105393037
API: 100164075
DNX: 107165075
ZNE: 110831721
FCD: 110848563
TUT: 107364932
CEL: CELE_Y39A1A.14(Y39A1A.14)
CBR: CBG18338
BMY: Bm1_42740
TSP: Tsp_04549
CRG: 105322332
MYI: 110463073
OBI: 106867204
LAK: 106175213
SHX: MS3_01507
EPA: 110252361
ADF: 107332954
HMG: 100209283
AQU: 100639345
ATH: AT3G57000
CRB: 111830519
BRP: 103841604
BOE: 106309854
CIT: 102606709
VRA: 106756151
VAR: 108339570
CAM: 101499888
LJA: Lj0g3v0059639.1(Lj0g3v0059639.1) Lj0g3v0059639.2(Lj0g3v0059639.2) Lj6g3v1422200.1(Lj6g3v1422200.1)
FVE: 101305881
CSV: 101219867
CMAX: 111482341
CMOS: 111442430
CPEP: 111790937
RCU: 8258367
JCU: 105634419
SLY: 101260019
SOT: 102578568
INI: 109182399
LSV: 111918100
NNU: 104600899
OSA: 4329057
DOSA: Os02t0290400-01(Os02g0290400)
OBR: 102706254
BDI: 100844288
ATS: 109734717(LOC109734717) 109781927(LOC109781927)
ZMA: 100282562
SITA: 101763420
PDA: 103701962
EGU: 105056316
AOF: 109829006
ATR: 18427279
PPP: 112276962
CRE: CHLREDRAFT_178371(NEP1)
MNG: MNEG_5650
APRO: F751_6608
SCE: YLR186W(EMG1)
ERC: Ecym_1069
KMX: KLMA_10085(EMG1)
NCS: NCAS_0A00500(NCAS0A00500)
NDI: NDAI_0F04300(NDAI0F04300)
TPF: TPHA_0B04640(TPHA0B04640)
TBL: TBLA_0A05270(TBLA0A05270)
TDL: TDEL_0C06380(TDEL0C06380)
KAF: KAFR_0D04030(KAFR0D04030)
CAL: CAALFM_C111380WA(NEP1)
CAUR: QG37_03721
SLB: AWJ20_1441(EMG1)
NCR: NCU02428
NTE: NEUTE1DRAFT85901(NEUTE1DRAFT_85901)
MGR: MGG_03587
MAW: MAC_00120
MAJ: MAA_04480
CMT: CCM_01212
MBE: MBM_03053
ANI: AN2759.2
ANG: ANI_1_1370094(An11g10540)
PBN: PADG_12027(PADG_06014)
ABE: ARB_07234
TVE: TRV_03942
PTE: PTT_13441
SPO: SPAC18G6.07c(mra1)
CNE: CNJ01990
CNB: CNBJ1480
ABP: AGABI1DRAFT35322(AGABI1DRAFT_35322)
ABV: AGABI2DRAFT67862(AGABI2DRAFT_67862)
MGL: MGL_2295
DDI: DDB_G0272732(emg1)
DFA: DFA_10447(emg1)
EHI: EHI_044610(3.t00130)
PCB: PCHAS_071840(PC000338.04.0) PCHAS_144240(PC301062.00.0)
TAN: TA18685
TPV: TP01_0556
BBO: BBOV_I002890(19.m02290) BBOV_III002060(17.m07199)
CPV: cgd4_2840
SMIN: v1.2.003758.t1(symbB.v1.2.003758.t1)
SPAR: SPRG_07725
MJA: MJ_0557
MTP: Mthe_0188
MFV: Mfer_0273
MKA: MK0202(mra1)
AFU: AF_0734
FPL: Ferp_0144
GAC: GACE_1894
GAH: GAH_00763
TAC: Ta1124
TVO: TVG1248551(TVG1248551)
PTO: PTO0697
FAI: FAD_0243
CDIV: CPM_0725
PHO: PH1379(PH1379)
PAB: PAB1852
PFU: PF1262
PFI: PFC_05525
PYN: PNA2_1962
PYS: Py04_1208
TKO: TK0876
TON: TON_0428
TGA: TGAM_1419
TSI: TSIB_0244
THE: GQS_03060
THA: TAM4_1471
THM: CL1_0260
TLT: OCC_09656
THS: TES1_0146
TNU: BD01_0275
TEU: TEU_07100
PPAC: PAP_01645
ABI: Aboo_0915
APE: APE_0502
ACJ: ACAM_0388
SMR: Smar_1081
IHO: Igni_0919
DKA: DKAM_1117
TAG: Tagg_0926
IAG: Igag_0468
SSO: SSO2226
SOL: Ssol_0045
SSOA: SULA_0043
SSOL: SULB_0044
SSOF: SULC_0043
STO: STK_21290(nep1)
SAI: Saci_0034
SID: M164_0043
SII: LD85_0043
SIH: SiH_0043
SIR: SiRe_0042
SIC: SiL_0042
MSE: Msed_0747
MCN: Mcup_1351
AHO: Ahos_2315
PAI: PAE1979
PIS: Pisl_1803
PCL: Pcal_1590
PAS: Pars_2054
PYR: P186_0220
POG: Pogu_0070
TNE: Tneu_0820
CMA: Cmaq_0625
TUZ: TUZN_1775
TTN: TTX_1734
VDI: Vdis_1824
VMO: VMUT_0265
TPE: Tpen_0557
ASC: ASAC_1404
NMR: Nmar_1625
NID: NPIRD3C_0427(nep)
NIN: NADRNF5_0255(nep)
NCT: NMSP_1568(nep1)
NEV: NTE_03110
NCV: NCAV_1676(nep)
NBV: T478_1339
NDV: NDEV_1977(nep)
KCR: Kcr_1323
BARC: AOA65_2162(nep1)
BARB: AOA66_1168(nep1)
LOKI: Lokiarch_13800(nep1)
 » show all
Taxonomy
Reference
1  [PMID:18208838]
  Authors
Taylor AB, Meyer B, Leal BZ, Kotter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wohnert J
  Title
The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.
  Journal
Nucleic. Acids. Res. 36 (2008) 1542-54.
  Sequence
[sce:YLR186W]
Reference
2  [PMID:20047967]
  Authors
Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kotter P, Engels JW, Heckel A, Karas M, Entian KD, Wohnert J
  Title
The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.
  Journal
Nucleic. Acids. Res. 38 (2010) 2387-98.
  Sequence
[hsa:10436] [mja:MJ_0557]
Reference
3  [PMID:20972225]
  Authors
Meyer B, Wurm JP, Kotter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wohnert J, Entian KD
  Title
The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Psi1191 in yeast 18S rRNA.
  Journal
Nucleic. Acids. Res. 39 (2011) 1526-37.
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.260
IUBMB Enzyme Nomenclature: 2.1.1.260
ExPASy - ENZYME nomenclature database: 2.1.1.260
BRENDA, the Enzyme Database: 2.1.1.260

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