KEGG   ENZYME: 2.1.1.263Help
Entry
EC 2.1.1.263                Enzyme                                 

Name
botryococcene C-methyltransferase;
TMT-3
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:botryococcene C-methyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + C30 botryococcene = 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene (overall reaction) [RN:R10199];
(1a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [RN:R10195];
(1b) S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [RN:R10196];
(2a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [RN:R10197];
(2b) S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [RN:R10198]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
C30 botryococcene [CPD:C20432];
3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [CPD:C20433];
20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [CPD:C20434]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [CPD:C20435];
3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [CPD:C20433];
20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [CPD:C20434]
Comment
Isolated from the green alga Botryococcus braunii BOT22. Shows a very weak activity with squalene.
History
EC 2.1.1.263 created 2012
Reference
1  [PMID:22241476]
  Authors
Niehaus TD, Kinison S, Okada S, Yeo YS, Bell SA, Cui P, Devarenne TP, Chappell J
  Title
Functional identification of triterpene methyltransferases from Botryococcus braunii race B.
  Journal
J. Biol. Chem. 287 (2012) 8163-73.
  Sequence
[up:H2E7T7]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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