KEGG   ENZYME: 2.1.1.270Help
Entry
EC 2.1.1.270                Enzyme                                 

Name
(+)-6a-hydroxymaackiain 3-O-methyltransferase;
HM3OMT;
HMM2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:(+)-6a-hydroxymaackiain 3-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain = S-adenosyl-L-homocysteine + (+)-pisatin [RN:R07750]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
(+)-6a-hydroxymaackiain [CPD:C16230]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
(+)-pisatin [CPD:C10516]
Comment
The protein from the plant Pisum sativum (garden pea) methylates (+)-6a-hydroxymaackiain at the 3-position. It also methylates 2,7,4'-trihydroxyisoflavanone on the 4'-position (cf. EC 2.1.1.212, 2,7,4-trihydroxyisoflavanone 4-O-methyltransferase) with lower activity.
History
EC 2.1.1.270 created 2013
Pathway
Isoflavonoid biosynthesis
Biosynthesis of secondary metabolites
Reference
1  [PMID:16667069]
  Authors
Preisig CL, Matthews DE, Vanetten HD
  Title
Purification and Characterization of S-Adenosyl-l-methionine:6a-Hydroxymaackiain  3-O-Methyltransferase from Pisum sativum.
  Journal
Plant. Physiol. 91 (1989) 559-66.
Reference
2  [PMID:9349277]
  Authors
Wu Q, Preisig CL, VanEtten HD
  Title
Isolation of the cDNAs encoding (+)6a-hydroxymaackiain 3-O-methyltransferase, the terminal step for the synthesis of the phytoalexin pisatin in Pisum sativum.
  Journal
Plant. Mol. Biol. 35 (1997) 551-60.
  Sequence
Reference
3  [PMID:17172354]
  Authors
Liu CJ, Deavours BE, Richard SB, Ferrer JL, Blount JW, Huhman D, Dixon RA, Noel JP
  Title
Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses.
  Journal
Plant. Cell. 18 (2006) 3656-69.
Reference
4  [PMID:17067644]
  Authors
Akashi T, VanEtten HD, Sawada Y, Wasmann CC, Uchiyama H, Ayabe S
  Title
Catalytic specificity of pea O-methyltransferases suggests gene duplication for (+)-pisatin biosynthesis.
  Journal
Phytochemistry. 67 (2006) 2525-30.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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