| Entry |
|
|---|
| Name |
tRNA (uracil54-C5)-methyltransferase;
transfer RNA uracil54 5-methyltransferase;
transfer RNA uracil54 methylase;
tRNA uracil54 5-methyltransferase;
m5U54-methyltransferase;
tRNA:m5U54-methyltransferase;
RUMT;
TrmA;
5-methyluridine54 tRNA methyltransferase;
tRNA(uracil-54,C5)-methyltransferase;
Trm2;
tRNA(m5U54)methyltransferase
|
|---|
| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
 |
|---|
| Sysname |
S-adenosyl-L-methionine:tRNA (uracil54-C5)-methyltransferase
|
|---|
| Reaction(IUBMB) |
S-adenosyl-L-methionine + uridine54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA [RN: R00594]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
uridine54 in tRNA
|
|---|
| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
5-methyluridine54 in tRNA
|
|---|
| Comment |
Unlike this enzyme, EC 2.1.1.74 (methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing)), uses 5,10-methylenetetrahydrofolate and FADH2 to supply the atoms for methylation of U54 [4].
|
|---|
| Orthology |
| tRNA (uracil-5-)-methyltransferase | | tRNA (uracil-5-)-methyltransferase |
|
|---|
| Genes |
HSA: | | PTR: | | PPS: | | GGO: | | PON: | | MCC: | | MMU: | | RNO: | | AML: | | SSC: | | ECB: | | MDO: | | SHR: | | OAA: | | GGA: | | TGU: | | XTR: | | DRE: | | TRU: | | OLA: | | API: | | SCE: | | AGO: | | ERC: | | KLA: | | LTH: | | PPA: | | VPO: | | ZRO: | | CGR: | | NCS: | | NDI: | | TPF: | | TBL: | | TDL: | | KAF: | | DHA: | | PIC: | | PGU: | | LEL: | | CAL: | | CTP: | | CDU: | | COT: | | YLI: | | CLU: | | NCR: | | SMP: | | PAN: | | TTT: | | MTM: | | MGR: | | FGR: | | NHE: | | VAL: | | SSL: | | ANI: | | NFI: | | AFM: | | AOR: | | ANG: | | AFV: | | ACT: | | PCS: | | CIM: | | CPW: | | PBL: | | URE: | | ABE: | | TVE: | | AJE: | | PNO: | | PTE: | | ZTR: | | TML: | | SPO: | | CNE: | | CNB: | | CGI: | | PPL: | | LBC: | | CCI: | | SCM: | | UMA: | | MGL: | | PGR: | | TGO: | | PTI: | | TPS: | | ECO: | | ECJ: | | ECD: | | EBW: | | ECOK: | | ECE: | | ECS: | | ECF: | | ETW: | | ELX: | | EOJ: | | EOI: | | EOH: | | ECG: | | EOK: | | ELR: | | ECC: | | ECP: | | ECI: | | ECV: | | ECX: | | ECW: | | ECM: | | ECY: | | ECR: | | ECQ: | | ECK: | | ECT: | | EOC: | | EUM: | | ECZ: | | ELO: | | ELN: | | ELH: | | ESE: | | ESO: | | ESM: | | ESL: | | ECL: | | EBR: | | EBD: | | EKO: | | EKF: | | EAB: | | EDH: | | EDJ: | | EIH: | | ENA: | | ELU: | | EUN: | | ELW: | | ELL: | | ELC: | | ELD: | | ELP: | | EBL: | | EBE: | | ELF: | | ECOA: | | EFE: | | EBT: | | STY: | | STT: | | SEX: | | STM: | | SEO: | | SEV: | | SEY: | | SEM: | | SEJ: | | SEB: | | SEF: | | SPT: | | SEK: | | SPQ: | | SEI: | | SEC: | | SEH: | | SHB: | | SEE: | | SEW: | | SEA: | | SED: | | SEG: | | SEL: | | SET: | | SENJ: | | SES: | | SBG: | | YPE: | | YPK: | | YPA: | | YPN: | | YPM: | | YPP: | | YPG: | | YPZ: | | YPT: | | YPD: | | YPX: | | YPH: | | YPS: | | YPI: | | YPY: | | YPB: | | YEN: | | YEP: | | YEY: | | SFL: | | SFX: | | SFV: | | SFE: | | SSN: | | SSJ: | | SBO: | | SBC: | | SDY: | | ECA: | | PCT: | | PCC: | | PWA: | | ETA: | | EPY: | | EPR: | | EAM: | | EAY: | | EBI: | | ERJ: | | PLU: | | PAY: | | SGL: | | ENT: | | ENC: | | ENO: | | ECLO: | | ESC: | | EEC: | | ENL: | | EAS: | | EAE: | | EAR: | | ESA: | | CSK: | | CTU: | | KPN: | | KPU: | | KPM: | | KPP: | | KPE: | | KPO: | | KVA: | | KOX: | | CKO: | | CRO: | | SPE: | | SRR: | | SRS: | | SRA: | | PMR: | | EIC: | | ETR: | | ETD: | | DDA: | | DDC: | | DDD: | | DZE: | | XBO: | | XNE: | | PAM: | | PLF: | | PAJ: | | PAQ: | | PVA: | | PAO: | | RAH: | | RAQ: | | RAA: | | PSI: | | HIN: | | HIT: | | HIP: | | HIF: | | HIL: | | HIU: | | HIE: | | HIZ: | | HDU: | | HAP: | | HPR: | | HSO: | | HSM: | | PMU: | | PMV: | | PUL: | | PMP: | | MSU: | | APL: | | APJ: | | APA: | | ASU: | | AAP: | | AAT: | | AAO: | | AAN: | | GAN: | | VCH: | | VCE: | | VCJ: | | VCO: | | VCR: | | VCM: | | VCI: | | VVU: | | VVY: | | VVM: | | VPA: | | VPB: | | VHA: | | VSP: | | VEX: | | VEJ: | | VFU: | | VFI: | | VFM: | | VSA: | | PPR: | | VAN: | | PAE: | | PAU: | | PAP: | | PAG: | | PDK: | | PPU: | | PPF: | | PPG: | | PPW: | | PST: | | PSB: | | PSP: | | PFL: | | PFO: | | PFS: | | PEN: | | PMY: | | PMK: | | PSA: | | PBA: | | CJA: | | AVN: | | ACI: | | ACD: | | ACB: | | ABM: | | ABY: | | ABC: | | ABN: | | ABB: | | SON: | | SDN: | | SFR: | | SAZ: | | SBL: | | SBM: | | SBN: | | SBP: | | SLO: | | SPC: | | SSE: | | SPL: | | SHE: | | SHM: | | SHN: | | SHW: | | SHL: | | SWD: | | SWP: | | SVO: | | ILO: | | CPS: | | PHA: | | PAT: | | PSM: | | SDE: | | AMC: | | ALT: | | GAG: | | PIN: | | TTU: | | FBL: | | TCX: | | TCY: | | CSA: | | HEL: | | MMW: | | MME: | | AHA: | | ASA: | | AVR: | | TAU: | | OCE: | | DNO: | | NMA: | | NME: | | NMC: | | NMN: | | NMI: | | NGO: | | NGK: | | NLA: | | EBA: | | AZO: | | DAR: | | TMZ: | | DSU: | | HHE: | | HMS: | | WSU: | | TDN: | | SKU: | | CJE: | | CJJ: | | CJU: | | CJN: | | CJR: | | CJD: | | CFF: | | CCV: | | CHA: | | CCO: | | CLA: | | ABU: | | ANT: | | SDL: | | NSA: | | NIS: | | SUN: | | NAM: | | LAS: | | BAH: | | BAI: | | BAL: | | BCB: | | BCG: | | BCQ: | | BTL: | | BSE: | | BCO: | | GTH: | | GWC: | | GYC: | | GYA: | | GCT: | | GMC: | | SPH: | | SPI: | | SPJ: | | SPK: | | SPA: | | SUB: | | LSL: | | CTC: | | MMO: | | AVA: | | DGE: | | TTH: | | TTJ: | |
» show all
 |
|---|
| Reference |
|
|---|
| Authors |
Bjork GR, Svensson I |
|---|
| Title |
Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae. |
|---|
| Journal |
Eur. J. Biochem. 9 (1969) 207-15. |
|---|
| Organism |
Saccharomyces cerevisiae [GN: sce] |
|---|
| Reference |
|
|---|
| Authors |
Greenberg R, Dudock B. |
|---|
| Title |
Isolation and chracterization of m5U-methyltransferase from Escherichia coli. |
|---|
| Journal |
J. Biol. Chem. 255 (1980) 8296-302. |
|---|
| Organism |
Escherichia coli [GN: eco] |
|---|
| Reference |
|
|---|
| Authors |
HURWITZ J, GOLD M, ANDERS M. |
|---|
| Title |
THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. 3. PURIFICATION OF SOLUBLE RIBONUCLEIC ACID-METHYLATING ENZYMES. |
|---|
| Journal |
J. Biol. Chem. 239 (1964) 3462-73. |
|---|
| Organism |
Escherichia coli [GN: eco] |
|---|
| Reference |
|
|---|
| Authors |
Delk AS, Nagle DP Jr, Rabinowitz JC. |
|---|
| Title |
Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2. |
|---|
| Journal |
J. Biol. Chem. 255 (1980) 4387-90. |
|---|
| Organism |
Streptococcus faecalis, Bacillus subtilis [GN: bsu] |
|---|
| Reference |
|
|---|
| Authors |
Kealey JT, Gu X, Santi DV. |
|---|
| Title |
Enzymatic mechanism of tRNA (m5U54)methyltransferase. |
|---|
| Journal |
Biochimie. 76 (1994) 1133-42. |
|---|
| Organism |
Escherichia coli [GN: eco] |
|---|
| Reference |
|
|---|
| Authors |
Gu X, Ivanetich KM, Santi DV. |
|---|
| Title |
Recognition of the T-arm of tRNA by tRNA (m5U54)-methyltransferase is not sequence specific. |
|---|
| Journal |
Biochemistry. 35 (1996) 11652-9. |
|---|
| Organism |
Escherichia coli [GN: eco] |
|---|
| Reference |
|
|---|
| Authors |
Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H. |
|---|
| Title |
Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs. |
|---|
| Journal |
J. Mol. Biol. 274 (1997) 505-18. |
|---|
| Organism |
Saccharomyces cerevisiae [GN: sce] |
|---|
| Reference |
|
|---|
| Authors |
Walbott H, Leulliot N, Grosjean H, Golinelli-Pimpaneau B |
|---|
| Title |
The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)-methyltransferase provides insights into its tRNA specificity. |
|---|
| Journal |
Nucleic. Acids. Res. 36 (2008) 4929-40. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 37257-02-6 |
|---|
