KEGG   ENZYME: 2.3.1.161Help
Entry
EC 2.3.1.161                Enzyme                                 

Name
lovastatin nonaketide synthase;
LNKS;
LovB;
LovC;
acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing, thioester-hydrolysing)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acyl-CoA:malonyl-CoA C-acyltransferase (dihydromonacolin L acid-forming)
Reaction(IUBMB)
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + 10 H+ + S-adenosyl-L-methionine + holo-[lovastatin nonaketide synthase] = dihydromonacolin L-[lovastatin nonaketide synthase] + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O [RN:R07251]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
malonyl-CoA [CPD:C00083];
NADPH [CPD:C00005];
H+ [CPD:C00080];
S-adenosyl-L-methionine [CPD:C00019];
holo-[lovastatin nonaketide synthase]
Product
dihydromonacolin L-[lovastatin nonaketide synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
S-adenosyl-L-homocysteine [CPD:C00021];
H2O [CPD:C00001]
Comment
This fungal enzyme system comprises a multi-functional polyketide synthase (PKS) and an enoyl reductase. The PKS catalyses many of the chain building reactions of EC 2.3.1.85, fatty-acid synthase, as well as a reductive methylation and a Diels-Alder reaction, while the reductase is responsible for three enoyl reductions that are necessary for dihydromonacolin L acid production.
History
EC 2.3.1.161 created 2002, modified 2015
Reference
1  [PMID:19900898]
  Authors
Ma SM, Li JW, Choi JW, Zhou H, Lee KK, Moorthie VA, Xie X, Kealey JT, Da Silva NA, Vederas JC, Tang Y
  Title
Complete reconstitution of a highly reducing iterative polyketide synthase.
  Journal
Science. 326 (2009) 589-92.
Reference
2  [PMID:10334994]
  Authors
Kennedy J, Auclair K, Kendrew SG, Park C, Vederas JC, Hutchinson CR.
  Title
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
  Journal
Science. 284 (1999) 1368-72.
Reference
3
  Authors
Auclair, K., Sutherland, A., Kennedy, J., Witter, D.J., van der Heever, J.P., Hutchinson, C.R. and Vederas, J.C.
  Title
Lovastatin nonaketide synthase catalyses an intramolecular Diels-Alder reaction of a substrate analogue.
  Journal
J. Am. Chem. Soc. 122 (2000) 11519-11520.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
235426-97-8

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