KEGG   ENZYME: 2.3.1.162Help
Entry
EC 2.3.1.162                Enzyme                                 

Name
taxadien-5alpha-ol O-acetyltransferase;
acetyl coenzyme A:taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:taxa-4(20),11-dien-5alpha-ol O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-4(20),11-dien-5alpha-yl acetate [RN:R06307]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
taxa-4(20),11-dien-5alpha-ol [CPD:C11895]
Product
CoA [CPD:C00010];
taxa-4(20),11-dien-5alpha-yl acetate
Comment
This is the third enzyme in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel), which is widely used in the treatment of carcinomas, sarcomas and melanomas.
History
EC 2.3.1.162 created 2002
Pathway
Diterpenoid biosynthesis
Orthology
K12923  
taxadien-5-alpha-ol O-acetyltransferase
Reference
1  [PMID:10190984]
  Authors
Walker K, Ketchum RE, Hezari M, Gatfield D, Goleniowski M, Barthol A, Croteau R.
  Title
Partial purification and characterization of acetyl coenzyme A: taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase that catalyzes the first acylation step of taxol biosynthesis.
  Journal
Arch. Biochem. Biophys. 364 (1999) 273-9.
Reference
2  [PMID:10666320]
  Authors
Walker K, Schoendorf A, Croteau R.
  Title
Molecular cloning of a taxa-4(20),11(12)-dien-5alpha-ol-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.
  Journal
Arch. Biochem. Biophys. 374 (2000) 371-80.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
229032-29-5

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