KEGG   ENZYME: 2.3.1.65Help
Entry
EC 2.3.1.65                 Enzyme                                 

Name
bile acid-CoA:amino acid N-acyltransferase;
glycine---taurine N-acyltransferase;
amino acid N-choloyltransferase;
BAT;
glycine N-choloyltransferase;
BACAT;
cholyl-CoA glycine-taurine N-acyltransferase;
cholyl-CoA:taurine N-acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
choloyl-CoA:glycine N-choloyltransferase
Reaction(IUBMB)
choloyl-CoA + glycine = CoA + glycocholate [RN:R03718]
Reaction(KEGG)
Substrate
choloyl-CoA [CPD:C01794];
glycine [CPD:C00037]
Product
CoA [CPD:C00010];
glycocholate [CPD:C01921]
Comment
Also acts on CoA derivatives of other bile acids. Taurine and 2-fluoro-beta-alanine can act as substrates, but more slowly [4]. The enzyme can also conjugate fatty acids to glycine and can act as a very-long-chain acyl-CoA thioesterase [7]. Bile-acid---amino-acid conjugates serve as detergents in the gastrointestinal tract, solubilizing long chain fatty acids, mono- and diglycerides, fat-soluble vitamins and cholesterol [4]. This is the second enzyme in a two-step process leading to the conjugation of bile acids with amino acids; the first step is the conversion of bile acids into their acyl-CoA thioesters, which is catalysed by EC 6.2.1.7, cholate---CoA ligase.
History
EC 2.3.1.65 created 1983, modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00430  Taurine and hypotaurine metabolism
ec01100  Metabolic pathways
Orthology
K00659  bile acid-CoA:amino acid N-acyltransferase
Genes
HSA: 570(BAAT)
PTR: 472997
PPS: 100972861 100992429(BAAT)
GGO: 101140183(BAAT)
PON: 100431907(BAAT)
NLE: 100598200(BAAT)
MCC: 100429419(BAAT)
MCF: 102132073(BAAT) 107127621
CSAB: 103219118(BAAT)
RRO: 104670797(BAAT)
RBB: 108537176(BAAT)
CJC: 100394190 100396712(BAAT)
MMU: 12012(Baat)
RNO: 29725(Baat)
CGE: 100756252(Baat) 100756541
HGL: 101716142(Baat) 101717293
CCAN: 109696243(Baat)
TUP: 102472797 102473647(BAAT)
CFA: 481635
AML: 100476252(BAAT)
FCA: 101095691(BAAT) 101096116
PTG: 102969591(BAAT) 102969870
AJU: 106972069(BAAT) 106972070
BTA: 530653 786798(BAAT)
BOM: 102268176(BAAT) 102268462
BIU: 109563300(BAAT)
PHD: 102325859 102326210(BAAT)
CHX: 102174570(BAAT)
CFR: 102523415(BAAT)
CDK: 105086911(BAAT)
BACU: 103006095 103017253(BAAT)
LVE: 103081315 103082496(BAAT)
OOR: 101284164(BAAT) 101284403
ECB: 100054567 100063137(BAAT)
EPZ: 103552469(BAAT) 103568036
HAI: 109395040(BAAT)
RSS: 109446124(BAAT)
PALE: 102892341(BAAT) 102897591
TMU: 101357199
MDO: 100023136(BAAT)
GGA: 769879(BAAT)
MGP: 100539658(BAAT)
CJO: 107314177
APLA: 101797844
ACYG: 106048282
GFR: 102037789
FAB: 101815920
PHI: 102101793
PMAJ: 107206148
FPG: 101924136
FCH: 102059453
CLV: 102094683
EGZ: 104134405(BAAT)
AAM: 106494986
CPIC: 101932563 101938826(BAAT)
ACS: 100566890(baat)
PVT: 110078385(BAAT)
PBI: 103065567(BAAT)
GJA: 107106123 107106126(BAAT)
XLA: 100137716(LOC100488333.L) 108707337
SRX: 107726829
SANH: 107689257
IPU: 108280978
OLA: 101166298
XMA: 102226402
PRET: 103465285
NFU: 107385610
HCQ: 109522368
ELS: 105020184
CIN: 100175018
CRG: 105343873
MYI: 110465077
OBI: 106881808
ADF: 107346883
 » show all
Taxonomy
Reference
1  [PMID:7372637]
  Authors
Czuba B, Vessey DA.
  Title
Kinetic characterization of cholyl-CoA glycine-taurine N-acyltransferase from bovine liver.
  Journal
J. Biol. Chem. 255 (1980) 5296-9.
Reference
2
  Authors
Jordan, T.W., Lee, R. and Lim, W.C.
  Title
Isoelectric focussing of soluble and particulate benzoyl-CoA and cholyl-CoA:amino acid N-acyltransferases from rat liver.
  Journal
Biochem. Int. 1 (1980) 325-330.
Reference
3  [PMID:422567]
  Authors
Vessey DA.
  Title
The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA:taurine-N-acyltransferase activities from bovine liver.
  Journal
J. Biol. Chem. 254 (1979) 2059-63.
Reference
4  [PMID:2037576]
  Authors
Johnson MR, Barnes S, Kwakye JB, Diasio RB.
  Title
Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver.
  Journal
J. Biol. Chem. 266 (1991) 10227-33.
  Sequence
[hsa:570]
Reference
5  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J. Lipid. Res. 43 (2002) 2062-71.
Reference
6  [PMID:12951368]
  Authors
He D, Barnes S, Falany CN.
  Title
Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization.
  Journal
J. Lipid. Res. 44 (2003) 2242-9.
  Sequence
[rno:29725]
Reference
7  [PMID:12810727]
  Authors
O'Byrne J, Hunt MC, Rai DK, Saeki M, Alexson SE.
  Title
The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.
  Journal
J. Biol. Chem. 278 (2003) 34237-44.
  Sequence
[hsa:570]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.65
IUBMB Enzyme Nomenclature: 2.3.1.65
ExPASy - ENZYME nomenclature database: 2.3.1.65
BRENDA, the Enzyme Database: 2.3.1.65
CAS: 65979-40-0

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