KEGG   ENZYME: 2.3.1.93Help
Entry
EC 2.3.1.93                 Enzyme                                 

Name
13-hydroxylupanine O-tigloyltransferase;
tigloyl-CoA:13-hydroxylupanine O-tigloyltransferase;
13-hydroxylupanine acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
(E)-2-methylcrotonoyl-CoA:13-hydroxylupanine O-2-methylcrotonoyltransferase
Reaction(IUBMB)
(E)-2-methylcrotonoyl-CoA + 13-hydroxylupanine = CoA + 13-[(E)-2-methylcrotonoyl]oxylupanine [RN:R04205]
Reaction(KEGG)
Substrate
(E)-2-methylcrotonoyl-CoA [CPD:C03345];
13-hydroxylupanine [CPD:C02621]
Product
CoA [CPD:C00010];
13-[(E)-2-methylcrotonoyl]oxylupanine
Comment
Benzoyl-CoA and, more slowly, pentanoyl-CoA, 3-methylbutanoyl-CoA and butanoyl-CoA can act as acyl donors. Involved in the synthesis of lupanine alkaloids.
History
EC 2.3.1.93 created 1986, modified 2011
Pathway
Tropane, piperidine and pyridine alkaloid biosynthesis
Orthology
K13610  
13-hydroxylupinine O-tigloyltransferase
Reference
1
  Authors
Wink, M. and Hartmann, T.
  Title
Enzymatic synthesis of quinolizidine alkaloid esters: a tigloyl-CoA:13-hydroxylupanine O-tigloyl transferase from Lupinus albus L.
  Journal
Planta 156 (1982) 560-565.
Reference
2
  Authors
Okada, T.. Hirai, M.Y., Suzuki, H., Yamazaki, M. and Saito, K.
  Title
Molecular characterization of a novel quinolizidine alkaloid O-tigloyltransferase: cDNA cloning, catalytic activity of recombinant protein and expression analysis in Lupinus plants.
  Journal
Plant Cell Physiol. 46 (2005) 233-244.
Reference
3  [PMID:8195240]
  Authors
Suzuki H, Murakoshi I, Saito K.
  Title
A novel O-tigloyltransferase for alkaloid biosynthesis in plants. Purification, characterization, and distribution in Lupinus plants.
  Journal
J. Biol. Chem. 269 (1994) 15853-60.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
85341-00-0

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