KEGG   ENZYME: 2.3.2.20Help
Entry
EC 2.3.2.20                 Enzyme                                 

Name
cyclo(L-leucyl-L-phenylalanyl) synthase;
AlbC;
cFL synthase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
L-leucyl-tRNALeu:L-phenylalanyl-tRNAPhe leucyltransferase (cyclizing)
Reaction(IUBMB)
L-leucyl-tRNALeu + L-phenylalanyl-tRNAPhe = tRNALeu + tRNAPhe + cyclo(L-leucyl-L-phenylalanyl) [RN:R10334]
Reaction(KEGG)
Substrate
L-leucyl-tRNA(Leu) [CPD:C02047];
L-phenylalanyl-tRNA(Phe) [CPD:C03511]
Product
tRNA(Leu) [CPD:C01645];
tRNA(Phe) [CPD:C01648];
cyclo(L-leucyl-L-phenylalanyl) [CPD:C20519]
Comment
The reaction proceeds following a ping-pong mechanism forming a covalent intermediate between an active site serine and the L-phenylalanine residue [2]. The protein, found in the bacterium Streptomyces noursei, also forms cyclo(L-phenylalanyl-L-phenylalanyl), cyclo(L-methionyl-L-phenylalanyl), cyclo(L-phenylalanyl-L-tyrosyl) and cyclo(L-methionyl-L-tyrosyl) [1].
History
EC 2.3.2.20 created 2013
Orthology
K21382  
cyclo(L-leucyl-L-phenylalanyl) synthase
Genes
SALU: 
SALL: 
STRE: 
Taxonomy
Reference
1  [PMID:19430487]
  Authors
Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, Tuphile K, Jacquet M, Braud S, Courcon M, Masson C, Dubois S, Lautru S, Lecoq A, Hashimoto S, Genet R, Pernodet JL
  Title
Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes.
  Journal
Nat. Chem. Biol. 5 (2009) 414-20.
  Sequence
Reference
2  [PMID:21296757]
  Authors
Sauguet L, Moutiez M, Li Y, Belin P, Seguin J, Le Du MH, Thai R, Masson C, Fonvielle M, Pernodet JL, Charbonnier JB, Gondry M
  Title
Cyclodipeptide synthases, a family of class-I aminoacyl-tRNA synthetase-like enzymes involved in non-ribosomal peptide synthesis.
  Journal
Nucleic. Acids. Res. 39 (2011) 4475-89.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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