KEGG   ENZYME: 2.4.1.150Help
Entry
EC 2.4.1.150                Enzyme                                 

Name
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase;
GCNT2 (gene name);
GCNT3 (gene name);
IGnT;
I-branching beta1,6-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->6-acetylglucosaminyltransferase;
Galbeta1->4GlcNAc-R beta1->6 N-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide beta-1,6-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminide 6-beta-N-acetylglucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R
Reaction(KEGG)
(other) R02790 R04654 R06013(G) R06189(G)
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R
Product
UDP [CPD:C00015];
beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-[beta-D-GlcNAc-(1->6)]-beta-D-Gal-(1->4)-beta-D-GlcNAc-R
Comment
The enzyme acts on poly-N-acetyllactosamine [glycan chains of beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine units connected by beta(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by beta(1,6)-linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.
History
EC 2.4.1.150 created 1984 (EC 2.4.1.164 created 1989, incorporated 2016), modified 2017
Pathway
Glycosphingolipid biosynthesis - lacto and neolacto series
Metabolic pathways
Orthology
K00742  
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase
Genes
HSA: 
2651(GCNT2)
PTR: 
462428(GCNT2)
PPS: 
100979555(GCNT6) 100983940(GCNT2)
GGO: 
PON: 
100173016(GCNT2)
NLE: 
MCC: 
697468(GCNT2)
MCF: 
CSAB: 
RRO: 
RBB: 
CJC: 
SBQ: 
MMU: 
14538(Gcnt2)
RNO: 
306860(Gcnt2) 689092(Gcnt6)
CGE: 
NGI: 
HGL: 
CCAN: 
OCU: 
TUP: 
102485510(GCNT2)
CFA: 
488215(GCNT2)
AML: 
UMR: 
103664926(GCNT2)
FCA: 
PTG: 
102972600(GCNT2)
AJU: 
106972037(GCNT2)
BTA: 
BOM: 
BIU: 
PHD: 
CHX: 
100861326(GCNT2)
OAS: 
101106554(GCNT2)
SSC: 
CFR: 
CDK: 
BACU: 
LVE: 
ECB: 
100063291(GCNT2)
EPZ: 
EAI: 
106833434(GCNT2)
MYB: 
MYD: 
HAI: 
109377537(GCNT2)
RSS: 
109458559(GCNT2)
PALE: 
102877728(GCNT2)
LAV: 
MDO: 
100027080(GCNT2)
SHR: 
OAA: 
GGA: 
MGP: 
CJO: 
107309590(GCNT2)
APLA: 
ACYG: 
GFR: 
106631903(GCNT2)
FAB: 
107603337(GCNT2)
PMAJ: 
107215848(GCNT2)
CCW: 
109145064(GCNT2)
FPG: 
101918293(GCNT2)
FCH: 
102057507(GCNT2)
CLV: 
102086182(GCNT2)
AAM: 
106482558(GCNT2)
ASN: 
AMJ: 
102575341(GCNT2)
PSS: 
102458109(GCNT2)
CMY: 
CPIC: 
ACS: 
PVT: 
PBI: 
103065974(GCNT2)
GJA: 
107111763(GCNT2)
XLA: 
XTR: 
100038221(gcnt2) 100491539(gcnt2)
NPR: 
LCM: 
102366551(GCNT2)
CMK: 
BFO: 
SPU: 
APLC: 
SKO: 
LOA: 
HRO: 
LGI: 
CRG: 
OVI: 
NVE: 
ADF: 
AQU: 
GSL: 
 » show all
Taxonomy
Reference
1  [PMID:6219989]
  Authors
van den Eijnden DH, Winterwerp H, Smeeman P, Schiphorst WE.
  Title
Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.
  Journal
J. Biol. Chem. 258 (1983) 3435-7.
Reference
2  [PMID:6238026]
  Authors
Basu M, Basu S.
  Title
Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.
  Journal
J. Biol. Chem. 259 (1984) 12557-62.
Reference
3  [PMID:6490658]
  Authors
Piller F, Cartron JP, Maranduba A, Veyrieres A, Leroy Y, Fournet B
  Title
Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.
  Journal
J. Biol. Chem. 259 (1984) 13385-90.
Reference
4  [PMID:7579796]
  Authors
Bierhuizen MF, Maemura K, Kudo S, Fukuda M
  Title
Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
  Journal
Glycobiology. 5 (1995) 417-25.
Reference
5  [PMID:10092606]
  Authors
Ujita M, McAuliffe J, Suzuki M, Hindsgaul O, Clausen H, Fukuda MN, Fukuda M
  Title
Regulation of I-branched poly-N-acetyllactosamine synthesis. Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I.
  Journal
J. Biol. Chem. 274 (1999) 9296-304.
Reference
6  [PMID:9915862]
  Authors
Yeh JC, Ong E, Fukuda M
  Title
Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.
  Journal
J. Biol. Chem. 274 (1999) 3215-21.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
85638-40-0

DBGET integrated database retrieval system