KEGG   ENZYME: 2.4.1.161Help
Entry
EC 2.4.1.161                Enzyme                                 

Name
oligosaccharide 4-alpha-D-glucosyltransferase;
amylase III;
1,4-alpha-glucan:1,4-alpha-glucan 4-alpha-glucosyltransferase;
1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase;
alpha-1,4-transglucosylase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
(1->4)-alpha-D-glucan:(1->4)-alpha-D-glucan 4-alpha-D-glucosyltransferase
Reaction(IUBMB)
Transfers the non-reducing terminal alpha-D-glucose residue from a (1->4)-alpha-D-glucan to the 4-position of a free glucose or of a glucosyl residue at the non-reducing terminus of a (1->4)-alpha-D-glucan, thus bringing about the rearrangement of oligosaccharides
Comment
The enzyme acts on amylose, amylopectin, glycogen and maltooligosaccharides. No detectable free glucose is formed, indicating the enzyme does not act as a hydrolase. The enzyme from the bacterium Cellvibrio japonicus has the highest activity with maltotriose as a donor, and also accepts maltose [3], while the enzyme from amoeba does not accept maltose [1,2]. Oligosaccharides with 1->6 linkages cannot function as donors, but can act as acceptors [3]. Unlike EC 2.4.1.25, 4-alpha-glucanotransferase, this enzyme can transfer only a single glucosyl residue.
History
EC 2.4.1.161 created 1989, modified 2013
Orthology
K18820  
oligosaccharide 4-alpha-D-glucosyltransferase
Genes
CPS: 
PAT: 
AMC: 
AMH: 
AMAA: 
AMAL: 
AMAE: 
AMAO: 
AMAD: 
AMAI: 
AMAG: 
AMAC: 
AMB: 
AMG: 
AMK: 
GAG: 
GNI: 
GNIT_2014(malZ)
GPS: 
FBL: 
CJA: 
CJA_3248(agd31B)
SAGA: 
 » show all
Taxonomy
Reference
1  [PMID:2423097]
  Authors
Nebinger P.
  Title
Separation and characterization of four different amylases of Entamoeba histolytica. I. Purification and properties.
  Journal
Biol. Chem. Hoppe. Seyler. 367 (1986) 161-7.
Reference
2  [PMID:2423098]
  Authors
Nebinger P.
  Title
Separation and characterization of four different amylases of Entamoeba histolytica. II. Characterization of amylases.
  Journal
Biol. Chem. Hoppe. Seyler. 367 (1986) 169-76.
Reference
3  [PMID:23132856]
  Authors
Larsbrink J, Izumi A, Hemsworth GR, Davies GJ, Brumer H
  Title
Structural enzymology of Cellvibrio japonicus Agd31B protein reveals alpha-transglucosylase activity in glycoside hydrolase family 31.
  Journal
J. Biol. Chem. 287 (2012) 43288-99.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9000-92-4

DBGET integrated database retrieval system