KEGG   ENZYME: 2.4.1.201Help
Entry
EC 2.4.1.201                Enzyme                                 

Name
alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase;
MGAT4C (gene name);
N-acetylglucosaminyltransferase VI;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase VI;
uridine diphosphoacetylglucosamine-glycopeptide beta-1->4-acetylglucosaminyltransferase VI;
mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase;
GnTVI;
GlcNAc-T VI;
UDP-N-acetyl-D-glucosamine:2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->2)]-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05992]
Reaction(KEGG)
R05992(G);
(other) R04642
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
Requires a high concentration of Mn2+ for maximal activity. The enzyme, characterized from hen oviduct membranes, participates in the processing of N-glycans in the Golgi apparatus. It transfers GlcNAc in beta1-4 linkage to a D-mannose residue that already has GlcNAc residues attached at positions 2 and 6 by beta linkages. No homologous enzyme appears to exist in mammals.
History
EC 2.4.1.201 created 1992, modified 2001, modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K13748  alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase C
Genes
HSA: 25834(MGAT4C)
PTR: 467085(MGAT4C)
PPS: 100992134(MGAT4C)
GGO: 101151757(MGAT4C)
PON: 100455323(MGAT4C)
NLE: 100584226(MGAT4C)
MCC: 700450(MGAT4C)
MCF: 101866242(MGAT4C)
CSAB: 103238817(MGAT4C)
RRO: 104655963(MGAT4C)
RBB: 108535046(MGAT4C)
CJC: 100413583(MGAT4C)
SBQ: 101043109(MGAT4C)
MMU: 67569(Mgat4c)
RNO: 299756(Mgat4c)
CGE: 100760589(Mgat4c)
NGI: 103726602(Mgat4c)
HGL: 101725010(Mgat4c)
CCAN: 109700961(Mgat4c)
OCU: 100339976(MGAT4C)
TUP: 102467784(MGAT4C)
CFA: 475418(MGAT4C)
AML: 100465193(MGAT4C)
UMR: 103660061(MGAT4C)
ORO: 101369336(MGAT4C)
FCA: 109501701(MGAT4C)
PTG: 102971513(MGAT4C)
AJU: 106976669(MGAT4C)
BTA: 539661(MGAT4C)
BOM: 102269061(MGAT4C)
BIU: 109558955(MGAT4C)
PHD: 102325642(MGAT4C)
CHX: 102169257(MGAT4C)
OAS: 101108733(MGAT4C)
SSC: 414851(MGAT4C)
CFR: 102503714(MGAT4C)
CDK: 105084877(MGAT4C)
BACU: 103004161(MGAT4C)
LVE: 103080459(MGAT4C)
OOR: 101281932(MGAT4C)
ECB: 100051064(MGAT4C)
EPZ: 103558210(MGAT4C)
EAI: 106838333(MGAT4C)
MYB: 102253362(MGAT4C)
MYD: 102769462(MGAT4C)
HAI: 109387992(MGAT4C)
RSS: 109459955(MGAT4C)
PALE: 102882602(MGAT4C)
LAV: 100654846(MGAT4C)
TMU: 101345845
MDO: 100020942(MGAT4C)
SHR: 100927084 100932381(MGAT4C)
OAA: 100081053(MGAT4C)
GGA: 395415(MGAT4C) 427873(MGAT4C)
MGP: 100541955(MGAT4C) 100550363
CJO: 107311189(MGAT4C) 107324722
APLA: 101791726(MGAT4C) 101799353
ACYG: 106041520(MGAT4C) 106045877
TGU: 100228531(MGAT4C) 100232682
GFR: 102034112(MGAT4C) 102043367
FAB: 101809036(MGAT4C) 101815032
PHI: 102103698 102110314(MGAT4C)
PMAJ: 107205204(MGAT4C) 107214888
CCW: 104692465 104698425(MGAT4C)
FPG: 101914300(MGAT4C) 101916943
FCH: 102045721(MGAT4C) 102049618
CLV: 102088784(MGAT4C) 102089679
EGZ: 104121996 104132945(MGAT4C)
AAM: 106485644 106488510(MGAT4C)
XLA: 108710250(mgat4c.L) 108713740 108715280
XTR: 100486336(mgat4c) 100489718
NPR: 108785666 108792416(MGAT4C)
DRE: 100333333(mgat4c) 492796(zgc:101663)
IPU: 108263961(mgat4c) 108275769
AMEX: 103027198(mgat4c) 103036431
BPEC: 110155136(mgat4c) 110162118
LCM: 102346343 102347820(MGAT4C)
SPU: 580331
SKO: 100372236
FCD: 110856945
SMIN: v1.2.033022.t1(symbB.v1.2.033022.t1)
 » show all
Taxonomy
Reference
1  [PMID:2525556]
  Authors
Brockhausen I, Hull E, Hindsgaul O, Schachter H, Shah RN, Michnick SW, Carver JP.
  Title
Control of glycoprotein synthesis. Detection and characterization of a novel branching enzyme from hen oviduct, UDP-N-acetylglucosamine:GlcNAc beta 1-6 (GlcNAc beta 1-2)Man alpha-R (GlcNAc to Man) beta-4-N-acetylglucosaminyltransferase VI.
  Journal
J. Biol. Chem. 264 (1989) 11211-21.
Reference
2  [PMID:10903319]
  Authors
Taguchi T, Ogawa T, Inoue S, Inoue Y, Sakamoto Y, Korekane H, Taniguchi N.
  Title
Purification and characterization of UDP-GlcNAc: GlcNAcbeta 1-6(GlcNAcbeta 1-2)Manalpha 1-R [GlcNAc to Man]-beta 1, 4-N-acetylglucosaminyltransferase VI from hen oviduct.
  Journal
J. Biol. Chem. 275 (2000) 32598-602.
  Sequence
[gga:395415]
Reference
3  [PMID:10962001]
  Authors
Sakamoto Y, Taguchi T, Honke K, Korekane H, Watanabe H, Tano Y, Dohmae N, Takio K, Horii A, Taniguchi N
  Title
Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)- manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI.
  Journal
J. Biol. Chem. 275 (2000) 36029-34.
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.201
IUBMB Enzyme Nomenclature: 2.4.1.201
ExPASy - ENZYME nomenclature database: 2.4.1.201
BRENDA, the Enzyme Database: 2.4.1.201
CAS: 119699-68-2

DBGET integrated database retrieval system