KEGG   ENZYME: 2.4.1.214Help
Entry
EC 2.4.1.214                Enzyme                                 

Name
glycoprotein 3-alpha-L-fucosyltransferase;
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase;
GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase;
GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fucalpha1->6GlcNAc)-Asn-peptide) alpha1->3-fucosyltransferase;
GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of 4-N-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 3-alpha-L-fucosyl-transferase;
GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 3-alpha-L-fucosyl-transferase;
GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 3-alpha-L-fucosyl-transferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
GDP-beta-L-fucose:N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] 3-alpha-L-fucosyltransferase (configuration-retaining)
Reaction(IUBMB)
GDP-beta-L-fucose + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] = GDP + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc}-L-asparaginyl-[protein] [RN:R06015]
Reaction(KEGG)
R06015(G) > R09318(G);
(other) R11318(G)
Show
Substrate
GDP-beta-L-fucose [CPD:C00325];
N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
Product
GDP [CPD:C00035];
N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc}-L-asparaginyl-[protein]
Comment
Requires Mn2+. The enzyme transfers to N-linked oligosaccharide structures (N-glycans), generally with a specificity for N-glycans with one unsubstituted non-reducing terminal GlcNAc residue. This enzyme catalyses a reaction similar to that of EC 2.4.1.68, glycoprotein 6-alpha-L-fucosyltransferase, but transferring the L-fucosyl group from GDP-beta-L-fucose to form an alpha1,3-linkage rather than an alpha1,6-linkage. The {iupac/misc/glycp#3.5::N-glycan} products of this enzyme are present in plants, insects and some other invertebrates (e.g., Schistosoma, Haemonchus, Lymnaea).
History
EC 2.4.1.214 created 2001
Pathway
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00753  glycoprotein 3-alpha-L-fucosyltransferase
Genes
BFO: BRAFLDRAFT_118057 BRAFLDRAFT_126419 BRAFLDRAFT_148435 BRAFLDRAFT_157961 BRAFLDRAFT_158143 BRAFLDRAFT_168192 BRAFLDRAFT_196427 BRAFLDRAFT_217829 BRAFLDRAFT_228496 BRAFLDRAFT_249686 BRAFLDRAFT_273851 BRAFLDRAFT_82752 BRAFLDRAFT_82754 BRAFLDRAFT_87449 BRAFLDRAFT_91840
SPU: 105445519
APLC: 110976930 110978133 110978134 110980446 110981755 110982633 110989209 110989340 110990202 110990203
DME: Dmel_CG6869(FucTA)
DPO: Dpse_GA19918 Dpse_GA25723 Dpse_GA25724
DAN: Dana_GF23688
DER: Dere_GG15882
DPE: Dper_GL18621 Dper_GL24771
DSE: Dsec_GM25513
DSI: Dsimw501_GD14528(Dsim_GD14528)
MDE: 101901412
AAG: 5575371
AME: 100578168
BTER: 100642198
AEC: 105151786
ACEP: 105627764
HST: 105182309
PGC: 109853983
NVI: 100116394
BMOR: 101742412
PMAC: 106715499
PRAP: 110992454
CEL: CELE_K12H6.3(fut-4)
HMG: 100199046
ATH: AT1G49710(FUT12) AT3G19280(FUT11)
CPAP: 110813783
CIT: 102617014
TCC: 18607941
EGR: 104418734
VRA: 106768760
VAR: 108343376
CCAJ: 109793169
CAM: 101502044
ADU: 107469717
AIP: 107623116
LJA: Lj4g3v0598850.1(Lj4g3v0598850.1)
FVE: 101293079
PPER: 18766662
PMUM: 103335356
PAVI: 110771695
PXB: 103937988
ZJU: 107427081
RCU: 8275299
JCU: 105629829
POP: 7457535
JRE: 108988882
VVI: 100255469
BVG: 104904482
SOE: 110778319
OSA: 4345809
DOSA: Os08t0472600-01(Os08g0472600)
OBR: 102709390
BDI: 100834636
ATS: 109765104(LOC109765104)
SBI: 8056895
SITA: 101766834
AOF: 109849943
ATR: 18421358
SMO: SELMODRAFT_452935(FucTA)
PPP: 112288886
SMIN: v1.2.020659.t1(symbB.v1.2.020659.t1)
 » show all
Taxonomy
Reference
1  [PMID:11420147]
  Authors
Wilson IB, Rendic D, Freilinger A, Dumic J, Altmann F, Mucha J, Muller S, Hauser MT.
  Title
Cloning and expression of cDNAs encoding alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana.
  Journal
Biochim. Biophys. Acta. 1527 (2001) 88-96.
  Sequence
[ath:AT3G19280]
Reference
2  [PMID:11382750]
  Authors
Fabini G, Freilinger A, Altmann F, Wilson IB.
  Title
Identification of core alpha 1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster. Potential basis of the neural anti-horseadish peroxidase epitope.
  Journal
J. Biol. Chem. 276 (2001) 28058-67.
  Sequence
Reference
3  [PMID:10419500]
  Authors
Leiter H, Mucha J, Staudacher E, Grimm R, Glossl J, Altmann F.
  Title
Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans.
  Journal
J. Biol. Chem. 274 (1999) 21830-9.
  Sequence
[vra:106768760]
Reference
4  [PMID:10567717]
  Authors
van Tetering A, Schiphorst WE, van den Eijnden DH, van Die I.
  Title
Characterization of a core alpha1-->3-fucosyltransferase from the snail Lymnaea stagnalis that is involved in the synthesis of complex-type N-glycans.
  Journal
FEBS. Lett. 461 (1999) 311-4.
Reference
5  [PMID:1868856]
  Authors
Staudacher E, Altmann F, Glossl J, Marz L, Schachter H, Kamerling JP, Hard K, Vliegenthart JF.
  Title
GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1----6GlcNAc)-Asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.
  Journal
Eur. J. Biochem. 199 (1991) 745-51.
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.214
IUBMB Enzyme Nomenclature: 2.4.1.214
ExPASy - ENZYME nomenclature database: 2.4.1.214
BRENDA, the Enzyme Database: 2.4.1.214
CAS: 68247-53-0

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