KEGG   ENZYME: 2.4.1.229Help
Entry
EC 2.4.1.229                Enzyme                                 

Name
[Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase;
Skp1-HyPro GlcNAc-transferase;
UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase;
UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase;
UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase;
UDP-N-acetyl-D-glucosamine:[Skp1-protein]-hydroxyproline N-acetyl-D-glucosaminyl-transferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:[Skp1-protein]-trans-4-hydroxy-L-proline N-acetyl-alpha-D-glucosaminyl-transferase
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + [Skp1-protein]-trans-4-hydroxy-L-proline = UDP + [Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
[Skp1-protein]-trans-4-hydroxy-L-proline
Product
UDP [CPD:C00015];
[Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
Comment
Skp1 is a cytoplasmic and nuclear protein required for the ubiquitination of cell cycle regulatory proteins and transcriptional factors. In Dictyostelium Skp1 is modified by the linear pentasaccharide Galalpha1-6Galalpha1-L-Fucalpha1-2Galbeta1-3GlcNAc, which is attached to a hydroxyproline residue at position 143. This enzyme catalyses the first step in the building up of the pentasaccharide by attaching an N-acetylglucosaminyl group to the hydroxyproline residue. It requires dithiothreitol and a divalent cation for activity.
History
EC 2.4.1.229 created 2003, modified 2013
Orthology
K12244  
[Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase
Genes
VCN: 
CSL: 
DDI: 
DPP: 
DFA: 
DFA_01363(gnt1)
ACAN: 
TPS: 
EHX: 
GTT: 
NGR: 
Taxonomy
Reference
1  [PMID:12244115]
  Authors
Van Der Wel H, Morris HR, Panico M, Paxton T, Dell A, Kaplan L, West CM.
  Title
Molecular cloning and expression of a UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase that modifies Skp1 in the cytoplasm of dictyostelium.
  Journal
J. Biol. Chem. 277 (2002) 46328-37.
  Sequence
Reference
2  [PMID:10593934]
  Authors
Teng-Umnuay P, van der Wel H, West CM.
  Title
Identification of a UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase in the cytoplasm of Dictyostelium.
  Journal
J. Biol. Chem. 274 (1999) 36392-402.
  Sequence
Reference
3  [PMID:11886837]
  Authors
West CM, van der Wel H, Gaucher EA.
  Title
Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins.
  Journal
Glycobiology. 12 (2002) 17R-27R.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
256531-81-4

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