KEGG   ENZYME: 2.4.1.243Help
Entry
EC 2.4.1.243                Enzyme                                 

Name
6G-fructosyltransferase;
fructan:fructan 6G-fructosyltransferase;
1F(1-beta-D-fructofuranosyl)m sucrose:1F(1-beta-D-fructofuranosyl)nsucrose 6G-fructosyltransferase;
6G-FFT;
6G-FT;
6G-fructotransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase
Reaction(IUBMB)
[1-beta-D-fructofuranosyl-(2->1)-]m+1-alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n-alpha-D-glucopyranoside = [1-beta-D-fructofuranosyl-(2->1)-]m-alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n-beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >= 0)
Substrate
[1-beta-D-fructofuranosyl-(2->1)-]m+1-alpha-D-glucopyranoside;
[1-beta-D-fructofuranosyl-(2->1)-]n-alpha-D-glucopyranoside
Product
[1-beta-D-fructofuranosyl-(2->1)-]m-alpha-D-glucopyranoside;
[1-beta-D-fructofuranosyl-(2->1)-]n-beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >= 0)
Comment
Inulins are polysaccharides consisting of linear or branched D-fructofuranosyl chains attached to the fructosyl residue of sucrose by a beta(2->1) linkage. This enzyme catalyses the transfer of the terminal (2->1)-linked -D-fructosyl group of an inulin chain onto O-6 position of the glucose residue of another inulin molecule [1]. For example, if 1-kestose [1F-(beta-D-fructofuranosyl)sucrose] is both the donor and recipient in the reaction shown above, i.e., if m = 1 and n = 1, then the products will be sucrose and 6G-di-beta-D-fructofuranosylsucrose. In this notation, the superscripts F and G are used to specify whether the fructose or glucose residue of the sucrose carries the substituent. Alternatively, this may be indicated by the presence and/or absence of primes (see {iupac/2carb/36#362::http://www.chem.qmul.ac.uk/iupac/2carb/36.html#362}). Sucrose cannot be a donor substrate in the reaction (i.e. m cannot be zero) and inulin cannot act as an acceptor. Side reactions catalysed are transfer of a beta-D-fructosyl group between compounds of the structure 1F-(1-beta-D-fructofuranosyl)m-6G-(1-beta-D-fructofuranosyl)n sucrose, where m >= 0 and n = 1 for the donor, and m >= 0 and n >= 0 for the acceptor.
History
EC 2.4.1.243 created 2006
Orthology
K21352  
6(G)-fructosyltransferase
Reference
1
  Authors
Shiomi, N.
  Title
Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.).
  Journal
Carbohydr. Res. 96 (1981) 281-292.
Reference
2
  Authors
Shiomi, N.
  Title
Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase.
  Journal
Carbohydr. Res. 106 (1982) 166-169.
Reference
3
  Authors
Shiomi, N. and Ueno, K.
  Title
Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology.
  Journal
J. Appl. Glycosci. 51 (2004) 177-183.
Reference
4  [PMID:15720693]
  Authors
Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N.
  Title
Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis).
  Journal
New. Phytol. 165 (2005) 813-24.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
79633-28-6

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