KEGG   ENZYME: 2.4.1.245Help
Entry
EC 2.4.1.245                Enzyme                                 

Name
alpha,alpha-trehalose synthase;
trehalose synthase;
trehalose synthetase;
UDP-glucose:glucose 1-glucosyltransferase;
TreT;
PhGT;
ADP-glucose:D-glucose 1-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
NDP-alpha-D-glucose:D-glucose 1-alpha-D-glucosyltransferase
Reaction(IUBMB)
NDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + NDP [RN:R10525]
Reaction(KEGG)
Substrate
NDP-alpha-D-glucose [CPD:C15541];
D-glucose [CPD:C00031]
Product
alpha,alpha-trehalose [CPD:C01083];
NDP [CPD:C00454]
Comment
Requires Mg2+ for maximal activity [1]. The enzyme-catalysed reaction is reversible [1]. In the reverse direction to that shown above, the enzyme is specific for alpha,alpha-trehalose as substrate, as it cannot use alpha- or beta-paranitrophenyl glucosides, maltose, sucrose, lactose or cellobiose [1]. While the enzymes from the thermophilic bacterium Rubrobacter xylanophilus and the hyperthermophilic archaeon Pyrococcus horikoshii can use ADP-, UDP- and GDP-alpha-D-glucose to the same extent [2,3], that from the hyperthermophilic archaeon Thermococcus litoralis has a marked preference for ADP-alpha-D-glucose [1] and that from the hyperthermophilic archaeon Thermoproteus tenax has a marked preference for UDP-alpha-D-glucose [4].
History
EC 2.4.1.245 created 2008, modified 2013
Pathway
ec00500  Starch and sucrose metabolism
ec01100  Metabolic pathways
Orthology
K13057  trehalose synthase
Genes
MSQ: BKP64_04260
NHL: Nhal_1697 Nhal_3100
TEE: Tel_12660
GBI: PG2T_08535
SLIM: SCL_1383
GEO: Geob_2843(treT)
GEB: GM18_3944
PCA: Pcar_3111(treT)
PACE: A6070_00930
PEF: A7E78_09185
DRT: Dret_0427
MXA: MXAN_0781
CCX: COCOR_06731(treT) COCOR_07114(treT1)
HOH: Hoch_4094
SAT: SYN_02626
SFU: Sfum_2348
SAME: SAMCFNEI73_pB0408(treT)
MCG: GL4_1007
AFR: AFE_2083
TPZ: Tph_c26450(treT)
LPIL: LIP_3316
SALS: SLNWT_0587
ACTI: UA75_21495
ACAD: UA74_21015
ACTN: L083_2862
RXY: Rxyl_2973
CWO: Cwoe_5908
TRO: trd_0944
STI: Sthe_2106
TTR: Tter_1718
PUV: PUV_09960
TTF: THTE_2039
EMI: Emin_1550
TAI: Taci_0297
HTH: HTH_0760
TTK: TST_0833(treT)
TMA: TM0392
TMW: THMA_0397
TMQ: THMB_0397
TMX: THMC_0397
TPT: Tpet_0528
TRQ: TRQ2_0542
TNA: CTN_0277
TNP: Tnap_0184
TLE: Tlet_1841
TME: Tmel_1821
TAF: THA_843
THER: Y592_00670
FNO: Fnod_0451
KOL: Kole_0299
CEX: CSE_06690
NDE: NIDE3904
NMV: NITMOv2_3873(treT)
NJA: NSJP_2226(treT)
MTP: Mthe_0797
MEMA: MMAB1_0304(treT) MMAB1_0814(treT) MMAB1_1593(treT)
NMG: Nmag_3832
PHO: PH1035(PH1035)
PFU: PF1742
PFI: PFC_09885
PYS: Py04_1331
TSI: TSIB_0361
TLT: OCC_03547
THS: TES1_1927
TEU: TEU_03525
PPAC: PAP_05120
SMR: Smar_0085
SSO: SSO2609
SOL: Ssol_0419
SSOA: SULA_0413
SSOL: SULB_0415
SSOF: SULC_0413
STO: STK_04040
SAI: Saci_1827
SID: M164_2688
SII: LD85_3027
SIH: SiH_2660
SIR: SiRe_2596
SIC: SiL_2546
MSE: Msed_0129
MCN: Mcup_1929
AHO: Ahos_0030
PAI: PAE1278
PCL: Pcal_1359
PAS: Pars_0594
PYR: P186_2621
POG: Pogu_1761
TUZ: TUZN_0976
TTN: TTX_0217(treT)
TPE: Tpen_1592
 » show all
Taxonomy
Reference
1  [PMID:15364950]
  Authors
Qu Q, Lee SJ, Boos W.
  Title
TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis.
  Journal
J. Biol. Chem. 279 (2004) 47890-7.
  Sequence
[tlt:OCC_03547]
Reference
2  [PMID:15737605]
  Authors
Ryu SI, Park CS, Cha J, Woo EJ, Lee SB.
  Title
A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: molecular cloning and characterization.
  Journal
Biochem. Biophys. Res. Commun. 329 (2005) 429-36.
  Sequence
[pho:PH1035]
Reference
3  [PMID:18835983]
  Authors
Nobre A, Alarico S, Fernandes C, Empadinhas N, da Costa MS
  Title
A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT.
  Journal
J. Bacteriol. 190 (2008) 7939-46.
  Sequence
[rxy:Rxyl_2973]
Reference
4  [PMID:18483808]
  Authors
Kouril T, Zaparty M, Marrero J, Brinkmann H, Siebers B
  Title
A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: the unidirectional TreT pathway.
  Journal
Arch. Microbiol. 190 (2008) 355-69.
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.245
IUBMB Enzyme Nomenclature: 2.4.1.245
ExPASy - ENZYME nomenclature database: 2.4.1.245
BRENDA, the Enzyme Database: 2.4.1.245

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