KEGG   ENZYME: 2.4.1.264Help
Entry
EC 2.4.1.264                Enzyme                                 

Name
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase;
GumK
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase
Reaction(IUBMB)
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol = UDP + D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol [RN:R09732]
Reaction(KEGG)
Substrate
UDP-glucuronate [CPD:C00167];
D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol [CPD:C19855]
Product
UDP [CPD:C00015];
D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol [CPD:C19854]
Comment
The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).
History
EC 2.4.1.264 created 2011
Orthology
K13659  
2-beta-glucuronyltransferase
Genes
XFM: 
XFN: 
XFF: 
XFL: 
XFS: 
XCC: 
XCC2445(gumK)
XCB: 
XCA: 
XCP: 
XCR_2758(gumK)
XCV: 
XCV2778(gumK)
XAC: 
XAC2576(gumK)
XCI: 
XCT: 
XCU: 
XCN: 
XCW: 
XCR: 
XCM: 
XCF: 
XCJ: 
XFU: 
XAX: 
XACM_2558(gumK)
XAO: 
XOO: 
XOO3170(gumK)
XOM: 
XOO3010(XOO3010)
XOP: 
PXO_01401(gumK)
XOY: 
XOR: 
XOC_1876(gumK)
XOZ: 
XTN: 
PSD: 
CJA: 
CJA_0618(gt70A)
AZO: 
azo2235(gumK)
SIX: 
ARA: 
AVI: 
SJP: 
SYB: 
GDI: 
GDI2542(gumK)
GDJ: 
GXY: 
GLX_27750(gumK)
GXL: 
RCE: 
RC1_2535(gumK)
NOS: 
CALH: 
FBT: 
 » show all
Taxonomy
Reference
1  [PMID:9537354]
  Authors
Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Puhler A, Ielpi L
  Title
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.
  Journal
J. Bacteriol. 180 (1998) 1607-17.
Reference
2  [PMID:7683019]
  Authors
Ielpi L, Couso RO, Dankert MA
  Title
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris.
  Journal
J. Bacteriol. 175 (1993) 2490-500.
Reference
3  [PMID:18854951]
  Authors
Kim SY, Kim JG, Lee BM, Cho JY
  Title
Mutational analysis of the gum gene cluster required for xanthan biosynthesis in  Xanthomonas oryzae pv oryzae.
  Journal
Biotechnol. Lett. 31 (2009) 265-70.
  Sequence
[xop:PXO_01398]
Reference
4  [PMID:16946469]
  Authors
Barreras M, Bianchet MA, Ielpi L
  Title
Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required  for xanthan polysaccharide synthesis.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 62 (2006) 880-3.
  Sequence
Reference
5  [PMID:18596046]
  Authors
Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L
  Title
Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.
  Journal
J. Biol. Chem. 283 (2008) 25027-35.
  Sequence
Reference
6  [PMID:11841812]
  Authors
Vojnov AA, Bassi DE, Daniels MJ, Dankert MA
  Title
Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris.
  Journal
Carbohydr. Res. 337 (2002) 315-26.
Reference
7  [PMID:14736729]
  Authors
Barreras M, Abdian PL, Ielpi L
  Title
Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.
  Journal
Glycobiology. 14 (2004) 233-41.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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