KEGG   ENZYME: 2.4.1.288Help
Entry
EC 2.4.1.288                Enzyme                                 

Name
galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase;
GlfT2
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase
Reaction(IUBMB)
28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Substrate
UDP-alpha-D-galactofuranose [CPD:C03733];
beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Product
UDP [CPD:C00015];
[beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Comment
Isolated from Mycobacterium tuberculosis. The enzyme adds approximately twenty-eight galactofuranosyl residues with alternating 1->5 and 1->6 links forming a galactan domain with approximately thirty galactofuranosyl residues. Involved in the formation of the cell wall in mycobacteria.
History
EC 2.4.1.288 created 2012
Orthology
K16650  
galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Genes
MVR: 
HRB: 
MTU: 
Rv3808c(glfT2)
MTV: 
MTC: 
MRA: 
MRA_3848(glfT)
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
CFBS_4037(glfT)
MTL: 
MTO: 
MTD: 
UDA_3808c(glfT)
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
MBB: 
MBT: 
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MAF_38230(glfT)
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MLE: 
MLB: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MUL_4992(glfT)
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MMAR_5372(glfT)
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
ASD: 
CGL: 
NCgl2783(Cgl2882)
CGB: 
CGU: 
WA5_2783(GlfT)
CGT: 
CGS: 
CGG: 
CGM: 
cgp_3191(glfT)
CGJ: 
CGQ: 
CGX: 
CEF: 
CDI: 
CDP: 
CDH: 
CDT: 
CDE: 
CDR: 
CDA: 
CDZ: 
CDB: 
CDS: 
CDD: 
CDW: 
CDV: 
CJK: 
CUR: 
CUA: 
CAR: 
CKP: 
CPU: 
CPL: 
CPG: 
Cp316_2020(glfT1)
CPP: 
CPK: 
CPQ: 
CPX: 
CPZ: 
COR: 
COP: 
COD: 
COS: 
COI: 
COE: 
COU: 
CPSE: 
CPSU: 
CPSF: 
CRD: 
CRES_0527(glfT)
CUL: 
CUC: 
CUE: 
CUN: 
CUS: 
CUQ: 
CUZ: 
CUJ: 
CVA: 
CVAR_0241(glfT)
CHN: 
CCN: 
CTER: 
CMD: 
CAZ: 
CFN: 
CCG: 
CVT: 
CGY: 
CGLY_15105(glfT2)
CAX: 
CII: 
CUV: 
COA: 
CDO: 
CHM: 
CSX: 
CMQ: 
B840_11685(glfT2)
CKU: 
CCJ: 
CMV: 
CEI: 
CTED: 
CUT: 
CLW: 
CDX: 
CDES_12965(glfT2)
CSP: 
WM42_1635(glfT2)
NFA: 
NCY: 
NBR: 
NNO: 
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
REQ: 
RPY: 
RHB: 
RAV: 
RFA: 
A3L23_02021(glfT2_1) A3L23_02060(glfT2_2)
GBR: 
GPO: 
GOR: 
GOQ: 
TPR: 
SRT: 
CBQ: 
CMI: 
CMM_1021(wcqJ)
CMS: 
CMC: 
CMN_00984(wcqJ)
CMH: 
MTS: 
MIO: 
MIX: 
MIP: 
MCW: 
ART: 
ARR: 
ARM: 
ARI: 
ARE: 
AAQ: 
ARH: 
ARW: 
AAU: 
ACH: 
APN: 
PSUL: 
RSA: 
KRH: 
KRH_08980(glfT)
KPL: 
KFV: 
RMU: 
RDN: 
SATK: 
BCV: 
BFA: 
XCE: 
SKE: 
ARS: 
PFR: 
PFRE: 
NCA: 
PSIM: 
AER: 
TCU: 
GOB: 
MMAR: 
KRA: 
SEN: 
SVI: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
PDX: 
PSEA: 
PSEE: 
PSEH: 
PSEQ: 
PECQ: 
SESP: 
BN6_01660(gtuS2-3)
KAL: 
KPHY: 
BLO: 
BLA: 
BLC: 
BLT: 
BBB: 
BBC: 
BNM: 
BLV: 
BLW: 
BLS: 
BANI: 
BANL: 
BNI: 
BANM: 
BDE: 
BDP_2042(rfbF)
BDN: 
BBF: 
BBB_0064(glfT)
BTP: 
BKA: 
BPSP: 
BANG: 
BCAT: 
SIJ: 
PDO: 
LEN: 
CTHE: 
LFI: 
LFP: 
LEG: 
 » show all
Taxonomy
Reference
1  [PMID:18423586]
  Authors
Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL
  Title
Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase.
  Journal
Carbohydr. Res. 343 (2008) 2130-9.
  Sequence
[mtu:Rv3808c]
Reference
2  [PMID:19571009]
  Authors
May JF, Splain RA, Brotschi C, Kiessling LL
  Title
A tethering mechanism for length control in a processive carbohydrate polymerization.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 11851-6.
  Sequence
[mtu:Rv3808c]
Reference
3  [PMID:22707726]
  Authors
Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK
  Title
Tetrameric Structure of the GlfT2 Galactofuranosyltransferase Reveals a Scaffold  for the Assembly of Mycobacterial Arabinogalactan.
  Journal
J. Biol. Chem. 287 (2012) 28132-43.
  Sequence
[mtu:Rv3808c]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system