KEGG   ENZYME: 2.4.1.47Help
Entry
EC 2.4.1.47                 Enzyme                                 

Name
N-acylsphingosine galactosyltransferase;
UGT8 (gene name);
CGT (gene name);
UDP galactose-N-acylsphingosine galactosyltransferase;
uridine diphosphogalactose-acylsphingosine galactosyltransferase;
UDP-galactose:N-acylsphingosine D-galactosyltransferase;
UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase;
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-galactose:N-acylsphingosine beta-D-galactosyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-alpha-D-galactose + a ceramide = UDP + a beta-D-galactosylceramide [RN:R01500 R06277]
Reaction(KEGG)
R01500 R06277(G) > R04322;
(other) R10804
Show
Substrate
UDP-alpha-D-galactose [CPD:C00052];
ceramide [CPD:C00195]
Product
UDP [CPD:C00015];
beta-D-galactosylceramide
Comment
This membrane-bound, endoplasmic reticulum-located enzyme catalyses the last step in the synthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. It has a strong preference for ceramides that contain hydroxylated fatty acids.
History
EC 2.4.1.47 created 1972
Pathway
ec00565  Ether lipid metabolism
ec00600  Sphingolipid metabolism
ec01100  Metabolic pathways
Orthology
K04628  ceramide galactosyltransferase
Genes
HSA: 7368(UGT8)
PTR: 461446(UGT8)
PPS: 100985864(UGT8)
GGO: 101137553(UGT8)
PON: 100437492(UGT8)
NLE: 100607960(UGT8)
MCC: 702979(UGT8)
MCF: 102126400(UGT8)
CSAB: 103236153(UGT8)
RRO: 104675972(UGT8)
RBB: 108527094(UGT8)
CJC: 100396627(UGT8)
SBQ: 101036737(UGT8)
MMU: 22239(Ugt8a)
RNO: 50555(Ugt8)
CGE: 100772408
NGI: 103731259(Ugt8)
HGL: 101702896(Ugt8)
CCAN: 109677276(Ugt8)
OCU: 100341450(UGT8)
TUP: 102480796(UGT8)
CFA: 487910(UGT8)
AML: 100465291(UGT8)
UMR: 103661766(UGT8)
ORO: 101383800(UGT8)
FCA: 101097722(UGT8)
PTG: 102965984(UGT8)
AJU: 106977315(UGT8)
BTA: 281566(UGT8)
BOM: 102269259(UGT8)
BIU: 109560234(UGT8)
PHD: 102316197(UGT8)
CHX: 102174638(UGT8)
OAS: 101113069(UGT8)
SSC: 100511825(UGT8)
CFR: 102522568(UGT8)
CDK: 105089918(UGT8)
BACU: 103004850(UGT8)
LVE: 103073438(UGT8)
OOR: 101289765(UGT8)
ECB: 100072587(UGT8)
EPZ: 103557103(UGT8)
EAI: 106842868(UGT8)
MYB: 102252842(UGT8)
HAI: 109390271(UGT8)
RSS: 109461061(UGT8)
PALE: 102881397(UGT8)
LAV: 100665032(UGT8)
TMU: 101352658
MDO: 100010696(UGT8)
SHR: 100914231(UGT8) 100927545
OAA: 100076336(UGT8)
GGA: 374033(UGT8) 428949(UGT8L)
MGP: 100539821 100541199(UGT8)
CJO: 107313583(UGT8) 107315928
APLA: 101794817 101799126(UGT8)
ACYG: 106029878 106038627(UGT8)
TGU: 100228968 100231902(UGT8)
GFR: 102031520(UGT8) 102040819
FAB: 101807254 101809664(UGT8)
PHI: 102107687(UGT8) 102108262
PMAJ: 107203102(UGT8) 107206597
CCW: 104696845(UGT8) 104697059
FPG: 101914948(UGT8) 101924669
FCH: 102047748 102051681(UGT8)
CLV: 102089904(UGT8) 102097199
AAM: 106492128(UGT8) 106498896
ASN: 102382334(UGT8) 102384980
AMJ: 102574992 106737104(UGT8)
CMY: 102936934(UGT8) 102941238
CPIC: 101945157 101952125(UGT8)
ACS: 100551810(ugt8)
PVT: 110083901(UGT8)
PBI: 103055483(UGT8)
GJA: 107110289(UGT8) 107122753
XLA: 108706712(ugt8.S) 108710441(ugt8.L)
XTR: 779534(ugt8)
NPR: 108792209(UGT8)
DRE: 553508(ugt8)
IPU: 108260842(ugt8)
AMEX: 103043793(ugt8)
TRU: 101064956(ugt8)
LCO: 104929353(ugt8) 109137995
NCC: 104955311(ugt8) 104960613
MZE: 101473219(ugt8)
OLA: 101156303(ugt8)
XMA: 102221616(ugt8)
PRET: 103467322(ugt8)
NFU: 107375211(ugt8)
CSEM: 103383812(ugt8)
LCF: 108896622(ugt8)
HCQ: 109519902(ugt8)
BPEC: 110165331(ugt8)
SASA: 106595509
ELS: 105009093(ugt8)
SFM: 108924325(ugt8)
LCM: 102350700 102364207(UGT8)
OBI: 106870503
AQU: 105315706
THJ: 104821000
CIT: 102614673
VRA: 106769098
 » show all
Taxonomy
Reference
1  [PMID:5807218]
  Authors
Fujino Y, Nakano M.
  Title
Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose.
  Journal
Biochem. J. 113 (1969) 573-5.
Reference
2  [PMID:5793706]
  Authors
Morell P, Radin NS.
  Title
Synthesis of cerebroside by brain from uridine diphosphate galactose and ceramide containing hydroxy fatty acid.
  Journal
Biochemistry. 8 (1969) 506-12.
Reference
3  [PMID:5497154]
  Authors
Morell P, Costantino-Ceccarini E, Radin NS
  Title
The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty  acids.
  Journal
Arch. Biochem. Biophys. 141 (1970) 738-48.
Reference
4  [PMID:5090043]
  Authors
Basu S, Schultz AM, Basu M, Roseman S.
  Title
Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain.
  Journal
J. Biol. Chem. 246 (1971) 4272-9.
Reference
5  [PMID:762114]
  Authors
Akanuma H, Kishimoto Y
  Title
Synthesis of ceramides and cerebrosides containing both alpha-hydroxy and nonhydroxy fatty acids from lignoceroyl-CoA by rat brain microsomes.
  Journal
J. Biol. Chem. 254 (1979) 1050-60.
Reference
6  [PMID:7306007]
  Authors
Koul O, Jungalwala FB
  Title
UDP-galactose:ceramide galactosyltransferase of rat central-nervous-system myelin.
  Journal
Biochem. J. 194 (1981) 633-7.
Reference
7  [PMID:7694285]
  Authors
Schulte S, Stoffel W
  Title
Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 90 (1993) 10265-9.
Reference
8  [PMID:9748263]
  Authors
Sprong H, Kruithof B, Leijendekker R, Slot JW, van Meer G, van der Sluijs P
  Title
UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum.
  Journal
J. Biol. Chem. 273 (1998) 25880-8.
Reference
9  [PMID:15998297]
  Authors
Fewou SN, Bussow H, Schaeren-Wiemers N, Vanier MT, Macklin WB, Gieselmann V, Eckhardt M
  Title
Reversal of non-hydroxy:alpha-hydroxy galactosylceramide ratio and unstable myelin in transgenic mice overexpressing UDP-galactose:ceramide galactosyltransferase.
  Journal
J. Neurochem. 94 (2005) 469-81.
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.47
IUBMB Enzyme Nomenclature: 2.4.1.47
ExPASy - ENZYME nomenclature database: 2.4.1.47
BRENDA, the Enzyme Database: 2.4.1.47
CAS: 37277-56-8

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