KEGG   ENZYME: 2.4.1.47Help
Entry
EC 2.4.1.47                 Enzyme                                 

Name
N-acylsphingosine galactosyltransferase;
UGT8 (gene name);
CGT (gene name);
UDP galactose-N-acylsphingosine galactosyltransferase;
uridine diphosphogalactose-acylsphingosine galactosyltransferase;
UDP-galactose:N-acylsphingosine D-galactosyltransferase;
UDP-alpha-D-galactose:N-acylsphingosine D-galactosyltransferase;
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-galactose:N-acylsphingosine beta-D-galactosyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-alpha-D-galactose + a ceramide = UDP + a beta-D-galactosylceramide [RN:R01500 R06277]
Reaction(KEGG)
R01500 R06277(G) > R04322;
(other) R10804
Show
Substrate
UDP-alpha-D-galactose [CPD:C00052];
ceramide [CPD:C00195]
Product
UDP [CPD:C00015];
beta-D-galactosylceramide
Comment
This membrane-bound, endoplasmic reticulum-located enzyme catalyses the last step in the synthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. It has a strong preference for ceramides that contain hydroxylated fatty acids.
History
EC 2.4.1.47 created 1972
Pathway
Ether lipid metabolism
Sphingolipid metabolism
Metabolic pathways
Orthology
K04628  
ceramide galactosyltransferase
Genes
HSA: 
7368(UGT8)
PTR: 
461446(UGT8)
PPS: 
100985864(UGT8)
GGO: 
101137553(UGT8)
PON: 
NLE: 
100607960(UGT8)
MCC: 
702979(UGT8)
MCF: 
102126400(UGT8)
CSAB: 
103236153(UGT8)
RRO: 
104675972(UGT8)
RBB: 
108527094(UGT8)
CJC: 
100396627(UGT8)
SBQ: 
101036737(UGT8)
MMU: 
22239(Ugt8a)
RNO: 
50555(Ugt8)
CGE: 
NGI: 
103731259(Ugt8)
HGL: 
101702896(Ugt8)
CCAN: 
109677276(Ugt8)
OCU: 
100341450(UGT8)
TUP: 
102480796(UGT8)
CFA: 
487910(UGT8)
AML: 
100465291(UGT8)
UMR: 
103661766(UGT8)
FCA: 
101097722(UGT8)
PTG: 
102965984(UGT8)
AJU: 
106977315(UGT8)
BTA: 
281566(UGT8)
BOM: 
102269259(UGT8)
BIU: 
109560234(UGT8)
PHD: 
102316197(UGT8)
CHX: 
102174638(UGT8)
OAS: 
101113069(UGT8)
SSC: 
100511825(UGT8)
CFR: 
102522568(UGT8)
CDK: 
105089918(UGT8)
BACU: 
103004850(UGT8)
LVE: 
103073438(UGT8)
ECB: 
100072587(UGT8)
EPZ: 
103557103(UGT8)
EAI: 
106842868(UGT8)
MYB: 
102252842(UGT8)
HAI: 
109390271(UGT8)
RSS: 
109461061(UGT8)
PALE: 
102881397(UGT8)
LAV: 
100665032(UGT8)
MDO: 
100010696(UGT8)
SHR: 
OAA: 
100076336(UGT8)
GGA: 
374033(UGT8) 428949(UGT8L)
MGP: 
CJO: 
APLA: 
ACYG: 
TGU: 
GFR: 
FAB: 
PHI: 
PMAJ: 
CCW: 
FPG: 
FCH: 
CLV: 
AAM: 
ASN: 
AMJ: 
PSS: 
CMY: 
CPIC: 
ACS: 
100551810(ugt8)
PVT: 
110083901(UGT8)
PBI: 
103055483(UGT8)
GJA: 
XLA: 
108706712(ugt8.S) 108710441(ugt8.L)
XTR: 
779534(ugt8)
NPR: 
108792209(UGT8)
DRE: 
553508(ugt8)
SRX: 
SANH: 
SGH: 
IPU: 
108260842(ugt8)
AMEX: 
103043793(ugt8)
TRU: 
101064956(ugt8)
TNG: 
LCO: 
NCC: 
MZE: 
101473219(ugt8)
OLA: 
101156303(ugt8)
XMA: 
102221616(ugt8)
CSEM: 
103383812(ugt8)
LCF: 
108896622(ugt8)
HCQ: 
109519902(ugt8)
BPEC: 
110165331(ugt8)
SASA: 
ELS: 
105009093(ugt8)
SFM: 
108924325(ugt8)
LCM: 
CMK: 
BFO: 
APLC: 
SKO: 
DPX: 
HRO: 
OBI: 
LAK: 
EPA: 
ADF: 
TAD: 
AQU: 
VRA: 
MNG: 
CSL: 
SRE: 
 » show all
Taxonomy
Reference
1  [PMID:5807218]
  Authors
Fujino Y, Nakano M.
  Title
Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose.
  Journal
Biochem. J. 113 (1969) 573-5.
Reference
2  [PMID:5793706]
  Authors
Morell P, Radin NS.
  Title
Synthesis of cerebroside by brain from uridine diphosphate galactose and ceramide containing hydroxy fatty acid.
  Journal
Biochemistry. 8 (1969) 506-12.
Reference
3  [PMID:5497154]
  Authors
Morell P, Costantino-Ceccarini E, Radin NS
  Title
The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty  acids.
  Journal
Arch. Biochem. Biophys. 141 (1970) 738-48.
Reference
4  [PMID:5090043]
  Authors
Basu S, Schultz AM, Basu M, Roseman S.
  Title
Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain.
  Journal
J. Biol. Chem. 246 (1971) 4272-9.
Reference
5  [PMID:762114]
  Authors
Akanuma H, Kishimoto Y
  Title
Synthesis of ceramides and cerebrosides containing both alpha-hydroxy and nonhydroxy fatty acids from lignoceroyl-CoA by rat brain microsomes.
  Journal
J. Biol. Chem. 254 (1979) 1050-60.
Reference
6  [PMID:7306007]
  Authors
Koul O, Jungalwala FB
  Title
UDP-galactose:ceramide galactosyltransferase of rat central-nervous-system myelin.
  Journal
Biochem. J. 194 (1981) 633-7.
Reference
7  [PMID:7694285]
  Authors
Schulte S, Stoffel W
  Title
Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 90 (1993) 10265-9.
Reference
8  [PMID:9748263]
  Authors
Sprong H, Kruithof B, Leijendekker R, Slot JW, van Meer G, van der Sluijs P
  Title
UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum.
  Journal
J. Biol. Chem. 273 (1998) 25880-8.
Reference
9  [PMID:15998297]
  Authors
Fewou SN, Bussow H, Schaeren-Wiemers N, Vanier MT, Macklin WB, Gieselmann V, Eckhardt M
  Title
Reversal of non-hydroxy:alpha-hydroxy galactosylceramide ratio and unstable myelin in transgenic mice overexpressing UDP-galactose:ceramide galactosyltransferase.
  Journal
J. Neurochem. 94 (2005) 469-81.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37277-56-8

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