KEGG   ENZYME: 2.4.99.19Help
Entry
EC 2.4.99.19                Enzyme                                 

Name
undecaprenyl-diphosphooligosaccharide---protein glycotransferase;
PglB
Class
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
BRITE hierarchy
Sysname
tritrans,heptacis-undecaprenyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase
Reaction(IUBMB)
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine
Substrate
tritrans,heptacis-undecaprenyl diphosphooligosaccharide;
[protein]-L-asparagine
Product
tritrans,heptacis-undecaprenyl diphosphate [CPD:C19852];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine
Comment
A bacterial enzyme that has been isolated from Campylobacter jejuni [1] and Campylobacter lari [2]. It forms a glycoprotein by the transfer of a glucosyl-N-acetylgalactosaminyl-N,N'-diacetylbacillosamine (GalNAc2(Glc)GalNAc3diNAcBac) polysaccharide and related oligosaccharides to the side-chain of an L-asparagine residue in the sequence -Asp/Glu-Xaa-Asn-Xaa'-Ser/Thr- (Xaa and Xaa' not Pro) in nascent polypeptide chains. Requires Mn2+ or Mg2+. Occurs on the external face of the plasma membrane. The polyprenol involved is normally tritrans,heptacis-undecaprenol but a decaprenol is used by some species.
History
EC 2.4.99.19 created 2012
Orthology
K17251  
undecaprenyl-diphosphooligosaccharide---protein glycotransferase
Genes
SUA: 
SULR: 
CJE: 
Cj1126c(pglB)
CJB: 
CJJ: 
CJU: 
C8J_1066(wlaF)
CJN: 
CJI: 
CJSA_1068(pglB)
CJM: 
CJM1_1104(wlaF)
CJS: 
CJS3_1172(pglB)
CJP: 
CJEJ: 
CJEU: 
CJEN: 
CJEI: 
CJER: 
CJV: 
CJY: 
QZ67_01203(pglB_1) QZ67_01204(pglB_2)
CJQ: 
UC78_1083(pglB)
CJL: 
CJW: 
CJR: 
CJE1268(pglB)
CJD: 
CJZ: 
CJX: 
CFF: 
CFT: 
CFV: 
CFX: 
CFZ: 
CAMP: 
CFP: 
CCV: 
CHA: 
CCO: 
CLA: 
Cla_1253(pglB)
CLR: 
CLM: 
CLQ: 
CLN: 
CLL: 
CCOL: 
CCC: 
CCQ: 
CCF: 
CCY: 
CCOI: 
CCOF: 
CAJ: 
CIS: 
CINS_1218(pglB)
CVO: 
CVOL_1232(pglB)
CPEL: 
CPEL_1359(pglB)
CAMR: 
CSM: 
CSF: 
CGRA: 
CURE: 
SBA: 
SMUL: 
SLH: 
DDN: 
DEJ: 
 » show all
Taxonomy
Reference
1  [PMID:20007322]
  Authors
Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D
  Title
Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases.
  Journal
J. Biol. Chem. 285 (2010) 4941-50.
  Sequence
[cjr:CJE1268]
Reference
2  [PMID:21677752]
  Authors
Lizak C, Gerber S, Numao S, Aebi M, Locher KP
  Title
X-ray structure of a bacterial oligosaccharyltransferase.
  Journal
Nature. 474 (2011) 350-5.
  Sequence
[cla:Cla_1253]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system