KEGG   ENZYME: 2.5.1.113Help
Entry
EC 2.5.1.113                Enzyme                                 

Name
[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase;
CysM
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
Reaction(IUBMB)
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate [RN:R10610]
Reaction(KEGG)
Substrate
O-phospho-L-serine [CPD:C01005];
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH [CPD:C15814]
Product
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine [CPD:C20729];
phosphate [CPD:C00009]
Comment
A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
History
EC 2.5.1.113 created 2013
Orthology
K21148  
[CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase
Genes
MTU: 
Rv1336(cysM)
MTV: 
MTC: 
MT1377(cysM)
MRA: 
MRA_1344(cysM)
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTE: 
MTUR: 
CFBS_1421(cysM)
MTL: 
MTO: 
MTD: 
UDA_1336(cysM)
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
Mb1371(cysM)
MBB: 
BCG_1398(cysM)
MBT: 
JTY_1372(cysM)
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MAF_13600(cysM)
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MUL_0242(cysM)
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MCHE: 
MIZ: 
MMC: 
MKM: 
MJL: 
MMI: 
MMAR_4629(cysM)
MMAE: 
MRH: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
MYVA_4106(cysM)
MYN: 
NFA: 
NFA_10910(cysM)
NFR: 
NCY: 
NBR: 
NNO: 
NSL: 
NSR: 
RHA: 
ROP: 
ROP_11450(cysM)
ROA: 
RHW: 
GBR: 
GPO: 
GOR: 
GOQ: 
GTA: 
TPR: 
SCO: 
SCO2910(SCE19A.10c)
SMA: 
SAV_5164(cysM2)
SGR: 
SGB: 
WQO_12370(cysM)
SCB: 
SSX: 
SVL: 
SCT: 
SCAT_1941(cysM)
SCY: 
SFA: 
SBH: 
SBI_06684(cysM3)
SHY: 
SHO: 
SVE: 
SDV: 
SALB: 
SALS: 
STRP: 
SFI: 
SCI: 
SRC: 
SALU: 
SALL: 
SLV: 
SGU: 
SVT: 
STRE: 
SCW: 
SLD: 
SXI: 
STRM: 
STRC: 
SAMB: 
SPRI: 
SCZ: 
SCX: 
SRW: 
STRF: 
SLE: 
sle_43610(sle_43610)
SRN: 
A4G23_01928(cysM_1)
SPAV: 
SLC: 
STRT: 
SCLF: 
SGS: 
STSI: 
SLS: 
SNR: 
SPLU: 
STRD: 
SNW: 
SAUO: 
KSK: 
KSE_28250(cysM)
KAB: 
KAU: 
MPH: 
MLP_38890(cysM)
NCA: 
NDK: 
I601_2313(cysM)
KFL: 
PSIM: 
AER: 
TFU: 
NDA: 
NAL: 
B005_3251(cysM)
NGV: 
TCU: 
SRO: 
NOA: 
FRA: 
FRE: 
FRI: 
FAL: 
FRAAL1500(cysM)
FSY: 
ACE: 
NML: 
GOB: 
BSD: 
MMAR: 
SEN: 
SACE_1206(cysM)
SVI: 
AMD: 
AMED_7711(cysK)
AMN: 
AMM: 
AMES_7594(cysK)
AMZ: 
B737_7594(cysK)
AOI: 
AORI_6492(cysM)
AJA: 
AMQ: 
ALU: 
PDX: 
PSEA: 
PSEE: 
PSEH: 
PSEQ: 
PECQ: 
PHH: 
AMI: 
SESP: 
BN6_11660(cysM)
KAL: 
KPHY: 
LED: 
AHM: 
ACTI: 
ACAD: 
AHG: 
ALL: 
PMAD: 
STP: 
SAQ: 
MAU: 
MIL: 
VMA: 
AMS: 
ASE: 
ACPL_1167(cysM)
ACTN: 
AFS: 
PLK: 
CAI: 
SNA: 
TBI: 
AEY: 
PLAN: 
PLAK: 
PLIM: 
ABAI: 
CWO: 
AYM: 
MOX: 
DAMO_1028(cysK)
 » show all
Taxonomy
Reference
1  [PMID:18842002]
  Authors
O'Leary SE, Jurgenson CT, Ealick SE, Begley TP
  Title
O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are  substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.
  Journal
Biochemistry. 47 (2008) 11606-15.
  Sequence
[mtu:Rv1336]
Reference
2  [PMID:18771296]
  Authors
Jurgenson CT, Burns KE, Begley TP, Ealick SE
  Title
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis.
  Journal
Biochemistry. 47 (2008) 10354-64.
  Sequence
[mtu:Rv1336]
Reference
3  [PMID:18799456]
  Authors
Agren D, Schnell R, Oehlmann W, Singh M, Schneider G
  Title
Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria.
  Journal
J. Biol. Chem. 283 (2008) 31567-74.
  Sequence
[mtu:Rv1336]
Reference
4  [PMID:19101553]
  Authors
Agren D, Schnell R, Schneider G
  Title
The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity.
  Journal
FEBS. Lett. 583 (2009) 330-6.
  Sequence
[mtu:Rv1336]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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