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Entry
EC 2.5.1.77                 Enzyme                                 

Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase;
FO synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
5-amino-6-(D-ribitylamino)uracil:4-hydroxyphenylpyruvate, 4-methylphenol transferase
Reaction(IUBMB)
5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3 [RN:R09396]
Reaction(KEGG)
Substrate
5-amino-6-(D-ribitylamino)uracil [CPD:C04732];
3-(4-hydroxyphenyl)pyruvate [CPD:C01179];
S-adenosyl-L-methionine [CPD:C00019];
H2O [CPD:C00001]
Product
7,8-didemethyl-8-hydroxy-5-deazariboflavin [CPD:C19154];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
oxalate [CPD:C00209];
NH3 [CPD:C00014]
Comment
Binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable AdoMet molecule. The first stage of catalysis is reduction of the 2 AdoMet to produce 2 methionine and 2 5'-deoxyadenosin-5-yl radicals that extract a hydrogen from each of the substrates permitting the condensation of the two [1]. The overall reaction catalysed is the transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylaminopyrimidine-2,4(1H,3H)-dione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO). 7,8-Didemethyl-8-hydroxy-5-deazariboflavin is the chromophore of the hydride carrier coenzyme F420 [1].
History
EC 2.5.1.77 created 2010
Pathway
Methane metabolism
Microbial metabolism in diverse environments
Orthology
K11780  
FO synthase subunit 1
K11781  
FO synthase subunit 2
Genes
BSUB: 
BSE: 
HHL: 
CTM: 
SYN: 
sll1285(cofG) sll1659(cofH)
SYZ: 
SYY: 
SYT: 
SYS: 
SYQ: 
SYC: 
syc0648_d(cofG) syc0843_c(cofG)
SYF: 
SYP: 
SYNE: 
TEL: 
tll1515(cofG) tlr1597(cofG)
THN: 
MAR: 
CYT: 
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
CAN: 
CSN: 
DSL: 
HAO: 
GLP: 
CMP: 
CYU: 
GVI: 
gll2100(cofG) glr0776(cofG)
GLJ: 
GKIL_0029(cofG) GKIL_2220(cofH)
ANA: 
alr3573(cofG) alr4129(cofG)
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
TER: 
Tery_0173(cofG) Tery_2116(cofG)
LEP: 
GEI: 
OAC: 
ONI: 
CEP: 
MIC: 
ARP: 
CTHE: 
PLP: 
SCS: 
MJA: 
MJ_0446(cofG) MJ_1431(cofG)
MFE: 
MVU: 
MFS: 
MIF: 
MIG: 
MMP: 
MMP0056(cofH) MMP0876(cofG)
MMQ: 
MMX: 
MMZ: 
MMD: 
MAE: 
Maeo_0750(cofG) Maeo_0911(cofG)
MVN: 
MVO: 
MOK: 
MAC: 
MA1490(cofG) MA1491(cofG)
MBA: 
MMA: 
MM_2494(cofG) MM_2495(cofG)
MMAZ: 
MBU: 
Mbur_1302(cofG) Mbur_1303(cofG)
MMH: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MCON_1058(cofG) MCON_3335(cofH)
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MCP_2457(cofH-2) MCP_2458(cofG)
MEZ: 
Mtc_0996(cofG) Mtc_0997(cofH-1) Mtc_0998(cofH-2)
RCI: 
RCIX459(cofH-2) RCIX460(cofH-1) RCIX461(cofG)
MTH: 
MMG: 
MST: 
Msp_0296(cofG) Msp_0392(cofH)
MSI: 
MRU: 
mru_1266(cofH) mru_1974(cofG)
MEB: 
Abm4_0772(cofH) Abm4_1547(cofG)
MEL: 
MEW: 
METH: 
MBMB1_0707(cofH1) MBMB1_1031(cofH3) MBMB1_1721(cofG)
MFV: 
MKA: 
MK0897(ThiH_1) MK0898(cofG)
AFU: 
APO: 
AVE: 
AST: 
FPL: 
HAL: 
VNG1937C(cofG)
HSL: 
OE3722F(cofG)
HMA: 
rrnAC3279(cofG)
HHI: 
HAH_0520(cofG)
HHN: 
HWA: 
HQ3125A(cofG)
HWC: 
Hqrw_3666(cofG)
NPH: 
NP2398A(cofG)
NMO: 
Nmlp_2441(cofG)
HLA: 
Hlac_1894(cofG)
HUT: 
Huta_1304(cofG)
HTI: 
HMU: 
Hmuk_1415(cofG)
HTU: 
NMG: 
HVO: 
HME: 
HFX_2258(thiH)
HJE: 
HBO: 
HXA: 
NAT: 
NPE: 
NGE: 
HRU: 
NOU: 
SALI: 
NMR: 
NKR: 
HAH: 
 » show all
Taxonomy
Reference
1  [PMID:14593448]
  Authors
Graham DE, Xu H, White RH
  Title
Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.
  Journal
Arch. Microbiol. 180 (2003) 455-64.
  Organism
Methanocaldococcus jannaschii [GN:mja]
  Sequence
Reference
2  [PMID:11948155]
  Authors
Choi KP, Kendrick N, Daniels L
  Title
Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis.
  Journal
J. Bacteriol. 184 (2002) 2420-8.
  Organism
Mycobacterium bovis [GN:mbo]
  Sequence
[mbo:Mb1206]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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