KEGG   ENZYME: 2.5.1.77Help
Entry
EC 2.5.1.77                 Enzyme                                 
Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase;
FO synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
5-amino-6-(D-ribitylamino)uracil:4-hydroxyphenylpyruvate, 4-methylphenol transferase
Reaction(IUBMB)
5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3 [RN:R09396]
Reaction(KEGG)
Substrate
5-amino-6-(D-ribitylamino)uracil [CPD:C04732];
3-(4-hydroxyphenyl)pyruvate [CPD:C01179];
S-adenosyl-L-methionine [CPD:C00019];
H2O [CPD:C00001]
Product
7,8-didemethyl-8-hydroxy-5-deazariboflavin [CPD:C19154];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
oxalate [CPD:C00209];
NH3 [CPD:C00014]
Comment
Binds a 4Fe-4S cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule. The first stage of catalysis is reduction of the 2 AdoMet to produce 2 methionine and 2 5'-deoxyadenosin-5-yl radicals that extract a hydrogen from each of the substrates permitting the condensation of the two [1]. The overall reaction catalysed is the transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylaminopyrimidine-2,4(1H,3H)-dione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO). 7,8-Didemethyl-8-hydroxy-5-deazariboflavin is the chromophore of the hydride carrier coenzyme F420 [1].
Pathway
Methane metabolism
Microbial metabolism in diverse environments
Orthology
K11780  
FO synthase subunit 1
K11781  
FO synthase subunit 2
Genes
BSUB: 
BSE: 
HHL: 
CTM: 
SYN: 
sll1285(cofG) sll1659(cofH)
SYZ: 
SYY: 
SYT: 
SYS: 
SYQ: 
SYC: 
syc0648_d(cofG) syc0843_c(cofG)
SYF: 
Synpcc7942_0686(cofG) Synpcc7942_0892(cofG)
SYP: 
SYNPCC7002_A1417(cofG) SYNPCC7002_A1920
SYNE: 
Syn6312_0669 Syn6312_2667
TEL: 
tll1515(cofG) tlr1597(cofG)
MAR: 
MAE_05370(cofG) MAE_44400
CYT: 
cce_2717(cofG) cce_4539
CYP: 
PCC8801_3080 PCC8801_3695(cofG)
CYC: 
PCC7424_0367(cofG) PCC7424_1434
CYN: 
Cyan7425_0829(cofG) Cyan7425_3281
CYH: 
Cyan8802_3040 Cyan8802_3749(cofG)
CYJ: 
Cyan7822_2858 Cyan7822_3246
CAN: 
Cyan10605_1120 Cyan10605_2647
CSN: 
Cyast_0246 Cyast_0748
DSL: 
Dacsa_0058 Dacsa_2777
HAO: 
PCC7418_0903 PCC7418_2609
GLP: 
Glo7428_1587 Glo7428_1689
CMP: 
Cha6605_0213 Cha6605_3166
CYU: 
UCYN_08790 UCYN_10830
GVI: 
gll2100(cofG) glr0776(cofG)
ANA: 
alr3573(cofG) alr4129(cofG)
NPU: 
Npun_F4799(cofG) Npun_R6205
NOS: 
Nos7107_3642 Nos7107_4537
NOP: 
Nos7524_0859 Nos7524_3594
AVA: 
Ava_0775 Ava_1598(cofG)
ANB: 
ANA_C11401(cofH) ANA_C12049(thiH)
ACY: 
Anacy_2798 Anacy_4497
NAZ: 
Aazo_0194 Aazo_0829
CSG: 
Cylst_3746 Cylst_4247
CALO: 
Cal7507_2039 Cal7507_3054
CALT: 
Cal6303_0714 Cal6303_4393
RIV: 
Riv7116_0978 Riv7116_1180
TER: 
Tery_0173(cofG) Tery_2116(cofG)
LEP: 
Lepto7376_2105 Lepto7376_3249
GEI: 
GEI7407_1065 GEI7407_2659
OAC: 
Oscil6304_4047 Oscil6304_5521
ONI: 
Osc7112_3345 Osc7112_4712
CEP: 
Cri9333_0183 Cri9333_1653
MIC: 
Mic7113_0382 Mic7113_5310
ARP: 
NIES39_K03090(cofG) NIES39_L02990(cofH)
CTHE: 
Chro_1933 Chro_4336
PLP: 
Ple7327_2924 Ple7327_4035
SCS: 
Sta7437_1936 Sta7437_2830
MJA: 
MJ_0446(cofG) MJ_1431(cofG)
MFE: 
Mefer_0989(cofG) Mefer_1391
MVU: 
Metvu_0187(cofG) Metvu_0742
MFS: 
MFS40622_0622 MFS40622_1713
MIF: 
Metin_0177 Metin_1060
MIG: 
Metig_0642 Metig_1294
MMP: 
MMP0056(cofH) MMP0876(cofG)
MMQ: 
MmarC5_0684(cofG) MmarC5_1621(cofG)
MMX: 
MmarC6_0893 MmarC6_1779(cofG)
MMZ: 
MmarC7_0124(cofG) MmarC7_1053(cofG)
MMD: 
GYY_00285 GYY_05090(cofG)
MAE: 
Maeo_0750(cofG) Maeo_0911(cofG)
MVN: 
Mevan_0044(cofG) Mevan_1071(cofG)
MVO: 
Mvol_0642 Mvol_1375
MOK: 
Metok_0201 Metok_1270
MAC: 
MA1490(cofG) MA1491(cofG)
MBA: 
Mbar_A3415 Mbar_A3416 Mbar_A3417
MMA: 
MM_2494(cofG) MM_2495(cofG)
MBU: 
Mbur_1302(cofG) Mbur_1303(cofG)
MMH: 
Mmah_1608 Mmah_1609
MEV: 
Metev_0265 Metev_0266
MZH: 
Mzhil_1604 Mzhil_1605
MPY: 
Mpsy_2558 Mpsy_2559(cofG)
MHZ: 
Metho_0950 Metho_0951 Metho_0952
MTP: 
Mthe_0221(cofG)
MCJ: 
MCON_1058(cofG) MCON_3335(cofH)
MHI: 
Mhar_0763 Mhar_0954
MHU: 
Mhun_1253
MLA: 
Mlab_1590
MEM: 
Memar_1838
MBG: 
BN140_0551(cofG)
MPI: 
Mpet_0714 Mpet_2579
MBN: 
Mboo_2201
MFO: 
Metfor_0519
MPL: 
Mpal_0976
MPD: 
MCP_2457(cofH-2) MCP_2458(cofG)
MEZ: 
Mtc_0996(cofG) Mtc_0997(cofH-1) Mtc_0998(cofH-2)
RCI: 
RCIX459(cofH-2) RCIX460(cofH-1) RCIX461(cofG)
MTH: 
MTH1198(cofG) MTH820
MMG: 
MTBMA_c12170(cofH) MTBMA_c15760(cofG)
MST: 
Msp_0296(cofG) Msp_0392(cofH)
MSI: 
Msm_0079 Msm_1035(cofG)
MRU: 
mru_1266(cofH) mru_1974(cofG)
MEL: 
Metbo_0140 Metbo_0327 Metbo_1931
MEW: 
MSWAN_0164 MSWAN_0688 MSWAN_2090
MFV: 
Mfer_0395 Mfer_0690
MKA: 
MK0897(ThiH_1) MK0898(cofG)
AFU: 
AF0797(cofG) AF0798
APO: 
Arcpr_1290 Arcpr_1291
AVE: 
Arcve_1610 Arcve_1611
AST: 
Asulf_02116 Asulf_02117
FPL: 
Ferp_0002 Ferp_0003
HAL: 
VNG1937C(cofG)
HSL: 
OE3722F(cofG)
HMA: 
rrnAC3279(cofG)
HHI: 
HAH_0520(cofG)
HWA: 
HQ3125A(cofG)
HWC: 
Hqrw_3666(cofG)
NPH: 
NP2398A(cofG)
NMO: 
Nmlp_2441(cofG)
HLA: 
Hlac_1894(cofG)
HUT: 
Huta_1304(cofG)
HMU: 
Hmuk_1415(cofG)
HTU: 
Htur_0642
NMG: 
Nmag_2633
HVO: 
HVO_2201
HME: 
HFX_2258(thiH)
HJE: 
HacjB3_04935(cofG)
HBO: 
Hbor_06390
HXA: 
Halxa_1896
NAT: 
NJ7G_3940
NPE: 
Natpe_3128
NGE: 
Natgr_1125
HRU: 
Halru_0972
NOU: 
Natoc_0463
NKR: 
NKOR_03090
HAH: 
Halar_2226
 » show all
Taxonomy
Reference
1  [PMID:14593448]
  Authors
Graham DE, Xu H, White RH
  Title
Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.
  Journal
Arch. Microbiol. 180 (2003) 455-64.
Reference
2  [PMID:11948155]
  Authors
Choi KP, Kendrick N, Daniels L
  Title
Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis.
  Journal
J. Bacteriol. 184 (2002) 2420-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system