KEGG   ENZYME: 2.5.1.96Help
Entry
EC 2.5.1.96                 Enzyme                                 

Name
4,4'-diapophytoene synthase;
dehydrosqualene synthase;
DAP synthase;
C30 carotene synthase;
CrtM
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
farnesyl-diphosphate:farnesyl-diphosphate farnesyltransferase (15-cis-4,4'-diapophytoene forming)
Reaction(IUBMB)
2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate (overall reaction) [RN:R07652];
(1a) 2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene diphosphate [RN:R00702];
(1b) presqualene diphosphate = 15-cis-4,4'-diapophytoene + diphosphate [RN:R09793]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
presqualene diphosphate [CPD:C03428]
Product
15-cis-4,4'-diapophytoene [CPD:C16144];
diphosphate [CPD:C00013];
presqualene diphosphate [CPD:C03428]
Comment
Requires Mn2+. Typical of Staphylococcus aureus and some other bacteria such as Heliobacillus sp.
History
EC 2.5.1.96 created 2011
Pathway
Carotenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K10208  
4,4'-diapophytoene synthase
Genes
SAU: 
SA2349(crtM)
SAV: 
SAV2562(crtM)
SAW: 
SAHV_2546(crtM)
SAH: 
SAJ: 
SAM: 
MW2483(crtM)
SAS: 
SAC: 
SACOL2577(crtM)
SAX: 
SAA: 
SAO: 
SAE: 
NWMN_2462(crtM)
SAD: 
SAAV_2628(crtM)
SUU: 
SUV: 
SUE: 
SUJ: 
SUK: 
SUC: 
SUT: 
SUQ: 
SUZ: 
MS7_2569(crtM)
SUD: 
SUX: 
SUW: 
SUG: 
SUF: 
SAUA: 
SAUE: 
SAUN: 
SAUS: 
SA40_2316(crtM)
SAUU: 
SAUG: 
SAUZ: 
SAUT: 
SAUJ: 
SAUK: 
SAUQ: 
SAUV: 
SAUW: 
SAUX: 
SAUY: 
SAB: 
SAB2435c(crtM)
SUY: 
SAUB: 
C248_2620(crtM)
SAUM: 
SAUC: 
SAUR: 
SAUI: 
SAUD: 
SAUF: 
X998_2545(crtM)
SHA: 
SH0490(crtM)
SHH: 
SCA: 
SLG: 
SLN: 
SWA: 
SPAS: 
SXY: 
SXL: 
SXO: 
SCAP: 
SEQO: 
SSIF: 
 » show all
Taxonomy
Reference
1  [PMID:12426357]
  Authors
Umeno D, Tobias AV, Arnold FH
  Title
Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway.
  Journal
J. Bacteriol. 184 (2002) 6690-9.
  Sequence
Reference
2  [PMID:16020541]
  Authors
Pelz A, Wieland KP, Putzbach K, Hentschel P, Albert K, Gotz F.
  Title
Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus.
  Journal
J. Biol. Chem. 280 (2005) 32493-8.
  Sequence
[sae:NWMN_2462]
Reference
3  [PMID:16269684]
  Authors
Ku B, Jeong JC, Mijts BN, Schmidt-Dannert C, Dordick JS
  Title
Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway.
  Journal
Appl. Environ. Microbiol. 71 (2005) 6578-83.
  Sequence
Reference
4  [PMID:18276850]
  Authors
Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E
  Title
A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence.
  Journal
Science. 319 (2008) 1391-4.
  Sequence
[sau:SA2349]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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